Richard J. Massey

Electrochemiluminescence-Based Detection System for the Quantitative Measurement of Antigens and Nucleic Acids: Application to HIV-1 and JC Viruses

A system for quantitative measurements based on electrochemiluminescence (ECL) has recently been developed. ECL is the generation of light through a series of chemical reactions at an electrode surface using a label that is a chelate of ruthenium(II) tris(2,2′-bipyridine) (Rubpy). ECL is ideally suited for analytic procedures involving antigens or nucleic acids because of the precision, sensitivity, and accuracy of the system. In addition, assay formats which eliminate wash steps and have rapid kinetics have been developed.