Richard Rasolofonirina
Université libre de Bruxelles
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Featured researches published by Richard Rasolofonirina.
Invertebrate Reproduction & Development | 2009
Aline Léonet; Richard Rasolofonirina; Ruddy Wattiez; Michel Jangoux; Igor Eeckhaut
Summary Oocyte maturation in sea cucumbers is stopped during the meiosis at prophase I. Maturation naturally concludes just before spawning leading to a mature oocyte ready to be fertilized. Although thermal shocks applied on mature individuals can induce spawning, it is currently not possible to perform reliably in vitro fertilization of sea cucumber oocytes. The present paper reports the discovery of a new and highly effective oocyte maturation inducer (OMI), called MIF (for maturation inducing fractions). MIFs effects on oocytes of the marketable species Holothuria scabra were analysed and compared to that of the few OMIs described in literature. MIF induces the maturation and fertilization of more than 90% of oocytes while other OMIs [1-methyladenine, dithiothreitol (DTT), dimercapto-propanol (BAL) and L-cysteine] induces 28–90% of oocytes to mature depending on the tested inducer. Moreover the use of the other OMIs result in fertilization rates that never exceed 40%, and the obtained larvae often resent developmental abnormalities. MIFs action on H. scabra oocytes is efficient yearlong even outside the spawning period of H. scabra. MIF is effective on the oocytes of all aspidochirote species tested so far but only when it is prepared from spawns of regular female sea urchin.
Aquaculture | 2004
Pierre Becker; David Gillan; Déborah Lanterbecq; Michel Jangoux; Richard Rasolofonirina; J. Rakotovao; Igor Eeckhaut
Marine Biology | 2003
Devarajen Vaïtilingon; Richard Rasolofonirina; Michel Jangoux
Western Indian Ocean journal of marine science | 2007
Richard Rasolofonirina; Devarajen Vaïtilingon; Igor Eeckhaut; Michel Jangoux
Bêche-de-mer | 2008
Igor Eeckhaut; Thierry Lavitra; Richard Rasolofonirina; M.W. Rabenevanana; P. Gildas; Michel Jangoux
SPC Beche-de-mer Information Bulletin | 2009
Thierry Lavitra; Richard Rasolofonirina; Michel Jangoux; Igor Eeckhaut
Archive | 2001
Michel Jangoux; Richard Rasolofonirina; Devarajen Vaïtilingon; Jean-Marc Ouin; Guy Seghers; Edouard Mara
Bêche-de-mer | 2008
Thierry Lavitra; Dina Rachelle; Richard Rasolofonirina; Michel Jangoux; Igor Eeckhaut
Western Indian Ocean journal of marine science | 2010
Thierry Lavitra; Richard Rasolofonirina; Igor Eeckhaut
Western Indian Ocean journal of marine science | 2007
Devarajen Vaïtilingon; Richard Rasolofonirina; Michel Jangoux