Richard Rodzen

Development of a Reversible Vas Deferens Occlusive Device. III. Morphology of the Human and Dog Vas Deferens: A study with the Scanning Electron Microscope *

The luminal epithelium of the human and dog vas deferens was studies with a scanning electron microscope. Care had to be taken in preparing the vasa since the columnar cell layer was readily removed from the underlying basal cells and the basement membrane. The luminal surfaces of both dog and human vasa were covered with microvilli. Occassionally cytoplasmic extrusions were observed protruding from the center of a microvillus patch. Foreign cells present in 1 specimen formed spherical impressions on the villus surface. In accordance with previous reports with the transmission electron microscope 2 types of columnar cells were found. One was long narrow and straight; the other possesed a rounded upper portion and a narrow base (stalk). Both columnar cell types appeared to rest on the basement membrane with a single point of attachment. The basal (cuboidal) cells were evenly space using tentacles to adhere to each other and the basement membrane. This membrane had a smooth surface and separted the epithelial cells from the collagen fibers which were present in large amounts in the connective tissue surrounding the vasal lumen. From the observations it appears that the human and dog vas deferens are highly similar in structure.(AUTHORS MODIFIED)

Development of a Reversible Vas Deferens Occlusive Device: V. Flexible Prosthetic Devices *

In contrast to the results with rigid-ended prosthetic devices implanted in the vas deferens of the dog, effective sperm transport was obtained for prolonged periods of time with flexible-ended (all soft) devices. Such transport could be halted by device occlusion with clips or shuttle stem valves. Opening the devices resulted once again in the passage of spermatozoa, indicating that these devices may be potentially useful as reversible male contraceptives. More research is necessary to determine the cause of an overall decrease in sperm output after device implantations and to evaluate any adverse side effects of such devices. Dacron velour-covered suture rings on the devices permitted effective tissue ingrowth and prevented leakage or recanalization.