Rima Kregzdyte

Content of lead in human hair from people with various exposure levels in Lithuania

A study has been performed to estimate if hair lead (Pb) can be used assessing environmental and occupational exposure to the metal. From 1994-1997, 622 ceramic plant employees, 520 randomly selected controls from Kaunas without occupational exposure to Pb aged 18-64 years, and 380 10-13 year old children living in Kaunas participated in this study. The concentration of Pb in the hair samples was measured by atomic absorption spectrometry. The geometric mean of Pb in the hair of workers occupationally exposed to Pb was significantly higher than that in persons not exposed to the metal (7.6 microg/g, 95% CI = 6.5-8.7 for exposed workers, 3.2 microg/g, 95% CI = 2.8-3.6 for not exposed workers and 2.6 microg/g, 95% CI = 2.4-2.9 for randomly selected controls, p < 0.05). Pb content in hair of boys and girls was 2.9 microg/g (95% CI = 2.6-3.3) and 3.1 microg/g (95% CI = 2.6-3.6), respectively. Lead in hair was not related to gender. Multiple regression analysis in adults with age as a continuous variable showed a positive relationship between Pb in hair and age. Also different exposure levels were significantly related to Pb in hair. In men, a positive association was found between Pb in hair and smoking. The data obtained suggest that human hair could be used to identify lead exposure in epidemiological surveys.

The Association of Low-To-Moderate Alcohol Consumption with Breast Cancer Subtypes Defined by Hormone Receptor Status

Background Alcohol is a well-established risk factor for breast cancer, but pathways involved in alcohol-related breast carcinogenesis are not clearly defined. We examined the association between low-to-moderate alcohol intake and breast cancer subtypes by tumor hormone receptor status. Materials and Methods A hospital-based case-control study was performed in 585 cases and 1,170 controls. Information on alcohol intake and other risk factors was collected via a questionnaire. Logistic regression was used for analyses. All statistical tests were two-sided. Results The odds ratio of breast cancer was 1.75 (95% confidence interval [CI]: 1.21–2.53) in women who consumed ≤5 drinks/week, and 3.13 (95% CI: 1.81–5.43) in women who consumed >5 drinks/week, both compared with non-drinkers for ≥10 years, after adjustment for age and other confounders. The association of alcohol intake with estrogen receptor-positive breast cancer was stronger than with estrogen receptor-negative: the odds ratio per 1 category increase was 2.05 (95% CI: 1.49–2.82) and 1.29 (95% CI: 0.85–1.94) (P-heterogeneity = 0.07). There was no evidence of an interaction between alcohol intake and menopausal status (P = 0.19) in overall group; however, it was significant in estrogen receptor-positive breast cancer (P = 0.04). Conclusions Low-to-moderate alcohol intake is associated with the risk of estrogen receptor-positive breast cancer with the strongest association in postmenopausal women. Since alcohol intake is a modifiable risk factor of breast cancer, every woman should be informed and advised to control alcohol use.

Influence of Echinacea purpurea (L.) Moench extract on the toxicity of cadmium.

Abstract:  Echinacea purpurea (L.) Moench (EP) has many beneficial features, especially strengthening the immune system. Cadmium (Cd) is a ubiquitous metal and cumulative poison that may cause liver and kidney damage and the formation of neoplasia. In this article, the changes in organs and metabolism, the accumulation of various levels of Cd in tissues, and the effects of EP liquid extract on Cd‐induced changes in mice were investigated. Experiments were carried out on the white laboratory mice. Solution of different Cd and EP concentrations were given to drink and experiments were performed. The concentration of Cd in mice blood, liver, kidneys, heart, spleen, skeletal muscle was determined using an electrothermal graphite furnace atomic absorption spectrophotometer Perkin–Elmer/Zeeman 3030. Long time per os of extract of EP combined with Cd leads to a significant increase of Cd concentration in blood and investigated organs of experiment mice.

Assessment of a Questionnaire for Breast Cancer Case-Control Studies

The aim of the present study was to assess criterion validity and external reliability of a questionnaire on risk factors for breast cancer. Materials and Methods. Women with breast cancer diagnosis (the cases) (N=40) and matched individuals without cancer (the controls) (N=40) were asked to fill in a questionnaire twice: on a day of admission to hospital (Q1) and on a day before discharge (Q2), with a time interval of 4-6 days. The questionnaire included questions (N=150) on demographic and socioeconomic factors, diseases in the past, family history of cancer, womans health, smoking, alcohol use, diet, physical activity, and work environment. Criterion validity of the questionnaire Q2 relative to reference questionnaire Q1 was assessed with the Spearman correlation coefficient (SCC); external reliability of the questionnaire was measured in terms of the intraclass correlation coefficient (ICC). Statistical analysis was performed with SPSS 16. Results. The responses to most of the questions on socioeconomic factors, family history on cancer, female health, lifestyle risk factors (smoking, alcohol use, physical activity) correlated substantially in both the cases and the controls with SCC and ICC>0.7 (p<0.01). Statistically non significant relationships defined only between the responses on amount of beer the cases drank at the ages up to 25 years and 26-35 years as well as time of use of estrogen and estrogens-progestin during menopause by the cases. Moderate and substantial SCC and ICC were determined for different food items. Only the response of the cases on veal consumption did not correlate significantly. Conclusions. The questionnaire on breast cancer risk factors is valid and reliable for most of the questions included.

Assessment of the Effect of Echinacea purpurea (L.) Moench on Apoptotic and Mitotic Activity of Liver Cells during Intoxication by Cadmium

Abstract:  Cadmium (Cd2+) is an important industrial pollutant, although its mechanism of toxicity has not been completely clarified. Cd2+ is toxic to a wide range of organs and tissues, however, the primary target organs of Cd2+ toxicity are the liver and kidney. Echinacea purpurea stimulating one or another tread of the immune system stimulates the expression of immunoglobulins and interferons. The experiments were performed on white laboratory mice using intraperitoneal (i.p.) injections 0.05 LD50 amount of CdCl2 solution. Two groups of mice were injected by Echinacea purpurea liquid extract: one 0.05 LD50 and the other 0.1 LD50. In this article, the Cd2+ distribution in internal organs, its effect on the mitotic and apoptotic activity of liver cells, as well as effects of Echinacea purpurea liquid extract on Cd2+‐induced changes in mice were investigated. Cd2+ concentration in mice blood, liver, and kidney was detected by atomic absorption spectroscopy. Long‐term injections of extract of Echinacea purpurea combined with Cd2+Cl2 leads to the significant increase of Cd2+ concentration in blood and investigated organs of experimental mice. Mitotic and apoptotic activity of liver cells was expressed as the estimated number of mitotic and apoptotic liver cells in randomly selected reference areas in histological slide. Echinacea purpurea decreases the mitotic activity of liver cells induced by Cd2+ and increases apoptotic activity of the liver cells.

Association between lifetime exposure to passive smoking and risk of breast cancer subtypes defined by hormone receptor status among non-smoking Caucasian women.

Tobacco smoking is inconsistently associated with breast cancer. Although some studies suggest that breast cancer risk is related to passive smoking, little is known about the association with breast cancer by tumor hormone receptor status. We aimed to explore the association between lifetime passive smoking and risk of breast cancer subtypes defined by estrogen receptor and progesterone receptor status among non-smoking Caucasian women. A hospital-based case-control study was performed in 585 cases and 1170 controls aged 28–90 years. Information on lifetime passive smoking and other factors was collected via a self-administered questionnaire. Logistic regression was used for analyses restricted to the 449 cases and 930 controls who had never smoked actively. All statistical tests were two-sided. Adjusted odds ratio of breast cancer was 1.01 (95% confidence interval (CI): 0.72–1.41) in women who experienced exposure to passive smoking at work, 1.88 (95% CI: 1.38–2.55) in women who had exposure at home, and 2.80 (95% CI: 1.84–4.25) in women who were exposed at home and at work, all compared with never exposed regularly. Increased risk was associated with longer exposure: women exposed ≤ 20 years and > 20 years had 1.27 (95% CI: 0.97–1.66) and 2.64 (95% CI: 1.87–3.74) times higher risk of breast cancer compared with never exposed (Ptrend < 0.001). The association of passive smoking with hormone receptor-positive breast cancer did not differ from that with hormone receptor-negative breast cancer (Pheterogeneity > 0.05). There was evidence of interaction between passive smoking intensity and menopausal status in both overall group (P = 0.02) and hormone receptor-positive breast cancer group (P < 0.05). In Caucasian women, lifetime exposure to passive smoking is associated with the risk of breast cancer independent of tumor hormone receptor status with the strongest association in postmenopausal women.

The Relationship of Echinacea purpurea. to the Toxicity of Cadmium

ABSTRACT Cadmium (Cd) is a ubiquitous metal and cumulative poison that may cause liver and kidney damage and the formation of neoplasia. The experiments were performed on white laboratory mice using intraperitoneal injections of 0.05 LD50 of cadmium chloride solution. Two groups of mice were injected with Echinacea purpurea. (L.) Moench liquid extract: one 0.05 LD50 and the other 0.1 LD50. Cd concentration in mouse blood, kidneys, liver, spleen, heart, and skeletal muscles was detected by atomic absorption spectroscopy. Estimated mitotic index of liver cells reflected the mitotic activity in the liver. Mitotic index in liver cells increases under the Cd intoxication, but it remains unchanged after simultaneous injection of Cd and Echinacea purpurea. liquid extract. In this study, the changes in organs and metabolism, the accumulation of various levels of intraperitoneal Cd in tissues, and the effects of Echinacea purpurea. liquid extract on Cd-induced changes in mice were investigated.