Rita de Cássia Campbell Machado Botteon

Freqüência de anticorpos contra Borrelia burgdorferi em cães na região metropolitana do Rio de Janeiro

Lyme disease is a worldwide zoonosis caused by Borrelia burgdorferi (Spirochaetae) and transmitted through ticks (Ixodidae). Many species of domestic and wild animals are affected. The dog is an important reservoir in the domiciliary environment. The disease affects the joints and causes alterations in other organs. Blood serum samples from 143 dogs, living in the counties of Seropedica, Itaguai and Rio de Janeiro, were analyzed with the indirect ELISA test, to search for antibodies against Borrelia burgdorferi latu sensu. Average positive results were 48.25%, 41.9% in Seropedica, 4.9% in Itaguai and 1.4% in Rio de Janeiro. The titres varied from 1:400 to 1:6400. The prevalence was lower (p< 0.05) in dogs up to one year of age than in older dogs, with significant difference. Between males and females was no statistical difference with positives titres. The result of this survey should draw attention to the occurrence of Borrelia sp in the area studied, considering the importance of Lyme disease as an emerging zoonosis.

Seroprevalencia de babesia equi en tres diferentes sistemas de crianza de equinos: Rio de Janeiro, Brasil

Blood samples were collected by jugular venipuncture and periferic blood smears horses maintained in tree breed system: confined, semi confined and extensive. The blood of this animals was analyzed for immunofluorescent antibody test (IFAT) for B. equi, and the prevalence obtained were the 89.58%, 87.89% and 45.24%, in extensive system, on semi confined system and confined system, respectively. For the direct exam in blood smear, the values was 16.7%, 16.13% and 4.78%, respectively in extensive system, on semi confined system and confined system

Babesiose em cavalos atletas portadores

The aim of this work was to evaluate the clinical occurrence of babesiosis in carrier athletic horses. During 12 monts, 38 horses carriers of B. equi, identified for de imunofluorescent assay test, participants of one of the sporting modalities: Dressage (n=7); jumping (n=8); Eventing (n=11) and Pole (n=12), were evaluated. During this period, 11 clinical cases of babesiosis by B. equi were diagnosed. That corresponded an incidence of 28.9%. Clinical and hematological evaluation showed performance fall and anemia occurrence with decrease in the eritrocites values, globular volume, and hemoglobin concentration. In the convalescence period, six animal that presented clinical babesiosis got injured, with lameness, myalgias, lumbalgias or excoriation and one presented hock fracture. A significant interaction among clinical babesiosis and lameness (P= 0.0372) and performance fall (P=0.0009).

Infecção natural por hemoparasitos em bezerros submetidos à quimio-profilaxia aos 30 dias de idade

The tick-borne disease (TBD) brings great damages to cattle breeding. The most important etiologic agents are Babesia bigemina, B. bovis and Anaplasma marginale, being the tick Boophilus microplus the main vector. This work reports the occurrence of natural infection by hemoparasites of TBD in 36 calves with high ticks natural infestation submitted to chemoprophylaxis with 30 days year-old. The blood smears from animals of different ages were analized and were found B. bigemina (33.3%), B. bovis (11.1%) and A. marginale (13.9%). Six animals had clinical symptoms (16.7%) and one dead (2.8%). The number of clinical cases ocurred in consequence of an association of factors as high infestation of ticks and low passive immunity in period that calves had not developed enough active immunity.

Hemograma e bioquímica sérica auxiliar em bezerros mestiços neonatos e ocorrência de enfermidades

ABSTRACT Packed cell volume, hematimetry, hemoglobin, total leukocyte, total plasmatic protein, fibrinogen, iron, and ferritin values were evaluated in 26 calves on days 1, 15, 30, and 56 after birth. In addition, calves were also observed during the parturition date. The data were evaluated according to disorders noticed by daily monitoring. Seventeen calves (65.4%) exhibited clinical signs and nine did not get ill. The main illnesses were diarrhea and omphalitis. Erythrogram, leukogram, plasmatic proteins, and fibrinogen were within the reference ranges, although a meaningful variation was observed relating age and fibrinogen. Individually, seven (26.9%) calves were anemic at birth. Moreover, anemia was also noticed in eight (30.7%) five-day-old animals and 15 (68.2%) and 11 (42.3%) animals on days 15 and 30, respectively. The parameters recorded in the cows were within the medium range and close to the calves. Thirteen (54.2%) cows presented packed cell volume and/or hematimetry under the normal ranges. Plasmatic iron was normal to the cows (139µg/dL) and also to the calves (110µg/dL). On the other side, ferritin was normal for calves (476

Alteração do pH fecal de equinos pela administração de alcalinizantes orais

In horses, many diseases are associated with the consumption of diets with high levels of grain or nonstructural carbohydrates. Excess of these foods, often con promotes lactic fermentation and intestinal acidosis, causing colic and laminitis. The addition of alkalizing in the diet does not have the same effect on horses as compared to ruminants. This study aimed to use different alkalizing and associations between them, in an attempt to reverse the process and control of acidosis in the large intestine of horses. 20 animals underwent four treatments with a control, one with Al2(OH)3, Mg(OH)2, and finally a combination thereof. The animals were evaluated for changes in fecal pH and clinical changes resulting from the administered products such as occurrence of constipation or diarrhea, and abdominal discomfort. It is concluded that administration of magnesium hydroxide (Mg (OH)2 at a dose of 20 g / 100 kg body weight and the combination of magnesium hydroxide and aluminum hydroxide 20g Al (OH) 3 + 20 g Mg (OH) 2 / 100 kg live weight, were able to significantly (P<0,05) alter the fecal pH, 24 hours after administration, without digestive disorders or significant abdominal discomfort

Padronização de técnica manual para obtenção de plasma rico em plaquetas de bovino

For standardization of manual technique to obtain autologous platelet-rich plasma (PRP) in cattle with reduced cost (manual method) and good quality (ability to concentrate platelets, high level of growth factors and reduced contamination with leukocytes and erythrocytes), that may be used as a modulating agent of the immune response of cows chronically infected with various diseases, 450ml of whole blood from nine clinically and hematologically healthy cattle were collected in CPDA-1 bags and processed within four hours after collection. The blood was divided in aliquots to evaluate 8 protocols (P) of double centrifugation which varied as the speed and time of centrifugation. Platelet, erythrocytes and leukocytes counts in PRP were performed by manual method in a Neubauer chamber. The highest concentration of platelets was obtained in P5 (400g and 800g both for 10 min), followed by (p>0.05) P3 (120g e 473g ambos durante 10 min), P4 (300g e 640g durante 10 min cada), P6 (640g durante 10 min e 640g durante 5 min), P8 (640g durante 5 min e 120g durante 10 min) and P7 (720g and 720g both for 5 min) and different (p <0.05) than the protocols that had lower rates at P1 (120g to 240g, both for 5 minutes) and P2 (both 120g and 473g for 5 min). As for erythrocytes, P8, P7, P6, P5, P4 showed lower concentrations with higher values (p <0.05) observed in P3 and P2. Lesser values of leukocytes were found in P5, P6, P7 and P8 with the biggest value (p <0.05) obtained in P2. All protocols (P1 to P8) were efficient to concentrate platelets and the lowest value (3.65±0.79) was found in P1. Regarding TGF-β1, the P1 and P8 protocols demonstrated the highest values. According to results, P5 and P8 protocols showed the best results for production of PRP in bovine.

Peroxidação lipídica em cabras com anemia e doença respiratória tratadas com ferro por via parenteral

Aimed to evaluate the progression of the disease and oxidative damage in goats with anemia and respiratory disease by application of parenteral iron. Six goats, adults, with anemia and respiratory disease (coughing, sneezing and nasal discharge) were studied. The control group was composed of six adults goats, healthy. The goats of both groups after undergoing clinical evaluation received similar dose (0.5g) of ferric hydroxide in complex dextran intramuscularly. Blood samples collected in EDTA before the application of iron and 48 hours were taken for determination of concentration of thiobarbituric acid reactive substances (TBARS). In patients goats before the application of iron TBARS values were equivalent to the measured values in the control group before and after applying the iron (p>0.05). The values measured in patients goats were significantly higher (p<0.001) in the group of animals even after the application of iron and controls before and after application. The application of iron worsened the clinical condition of the animals with respiratory disease, evidencing a condition of toxicity reflected by oxidative stress. Therefore, one should not recommend to supplementation in goats with respiratory diseases.

Efeito da lactulose sobre os teores séricos de ureia, creatinina, cálcio e fósforo em cães não azotêmicos

The present study evaluated serum levels of urea, creatinine, calcium and phosphorus in non-azothemic dogs by continued use of lactulose orally. Serum levels of urea, creatinine, calcium and phosphorus were determined in Beagle dogs, clinically healthy and without biochemical changes (non-azothemic), undergoing oral treatment with lactulose (n = 6), for a period of 30 days. The prebiotic showed no significant lowering effect on serum urea and creatinine, but the values of calcium and phosphorus, as well as their relation, were modified with reduced serum phosphorus levels in animals treated with lactulose compared to controls, with a significant difference.