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Featured researches published by Rita de Cássia Saraiva Nunomura.


Memorias Do Instituto Oswaldo Cruz | 2007

In vitro inhibition of Plasmodium falciparum by substances isolated from Amazonian antimalarial plants

Valter Ferreira de Andrade-Neto; Adrian Martin Pohlit; Ana Cristina da Silva Pinto; Ellen Cristina Costa da Silva; Karla Lagos Nogueira; Marcia R. S. Melo; Marycleuma Campos Henrique; Rodrigo César das Neves Amorim; Luís Francisco Rocha Silva; Mônica Regina Farias Costa; Rita de Cássia Saraiva Nunomura; Sergio Massayoshi Nunomura; Wilson Duarte Alecrim; M. Das Gracas C. Alecrim; F. Célio M. Chaves; Pedro Paulo Vieira

In the present study, a quassinoid, neosergeolide, isolated from the roots and stems of Picrolemma sprucei (Simaroubaceae), the indole alkaloids ellipticine and aspidocarpine, isolated from the bark of Aspidosperma vargasii and A. desmanthum (Apocynaceae), respectively, and 4-nerolidylcatechol, isolated from the roots of Pothomorphe peltata (Piperaceae), all presented significant in vitro inhibition (more active than quinine and chloroquine) of the multi-drug resistant K1 strain of Plasmodium falciparum. Neosergeolide presented activity in the nanomolar range. This is the first report on the antimalarial activity of these known, natural compounds. This is also the first report on the isolation of aspidocarpine from A. desmanthum. These compounds are good candidates for pre-clinical tests as novel lead structures with the aim of finding new antimalarial prototypes and lend support to the traditional use of the plants from which these compounds are derived.


Journal of the Brazilian Chemical Society | 2009

Characterization of bergenin in Endopleura uchi bark and its anti-inflammatory activity

Rita de Cássia Saraiva Nunomura; Viviane Guedes de Oliveira; Saulo L. da Silva; Sergio Massayoshi Nunomura

Endopleura uchi (Huber) Cuatrec. is an Amazon species traditionally used for the treatment of inflammations and female disorders. Pure bergenin was isolated from the methanolic extract of bark of E. uchi, firstly by using liquid-liquid partition chromatography followed by column chromatography over Sephadex LH-20 and then silica gel 60 flash chromatography. The structure of bergenin was identified on the basis of its NMR spectra. The in vitro anti-inflammatory activity was determined by the measurement of the inhibitory concentration (IC) of bergenin against three key enzymes: COX-1, COX-2 (cyclooxygenases) and phospholipase A2 (PLA2). These enzymes were selected because they are important targets for the discovery of new anti-inflammatory drugs associated with the biosynthesis of prostaglandins. The IC50 of bergenin for phopholipase A2 was determined as 156.6 µmol L-1 and bergenin was not considered active as compared to the positive control, tioetheramide PC. Bergenin did not inhibit COX-1 as well (IC50 = 107.2 µmol L-1). However, bergenin selectively inhibited COX-2 (IC50 = 1.2 µmol L-1). Because of the use of E. uchi in traditional medicine, bergenin was quantified in teas prepared as prescribed in traditional medicine by RP-HPLC as being 3% in the bark of E. uchi. The inhibitory activity towards COX-2 is important, since selective inhibitors of COX-2 have been clinically validated as anti-inflammatory therapeutics due to their enhanced gastrointestinal safety.


Memorias Do Instituto Oswaldo Cruz | 2009

Biological activity of neosergeolide and isobrucein B (and two semi-synthetic derivatives) isolated from the Amazonian medicinal plant Picrolemma sprucei (Simaroubaceae)

Ellen Cristina Costa da Silva; Bruno C. Cavalcanti; Rodrigo César das Neves Amorim; Jorcilene F Lucena; Dulcimar S Quadros; Wanderli Pedro Tadei; Raquel Carvalho Montenegro; Letícia V. Costa-Lotufo; Cláudia Pessoa; Manoel Odorico de Moraes; Rita de Cássia Saraiva Nunomura; Sergio Massayoshi Nunomura; Marcia R. S. Melo; Valter Ferreira de Andrade-Neto; Luiz Francisco Rocha e Silva; Pedro Paulo Vieira; Adrian Martin Pohlit

In the present study, in vitro techniques were used to investigate a range of biological activities of known natural quassinoids isobrucein B (1) and neosergeolide (2), known semi-synthetic derivative 1,12-diacetylisobrucein B (3), and a new semi-synthetic derivative, 12-acetylneosergeolide (4). These compounds were evaluated for general toxicity toward the brine shrimp species Artemia franciscana, cytotoxicity toward human tumour cells, larvicidal activity toward the dengue fever mosquito vector Aedes aegypti, haemolytic activity in mouse erythrocytes and antimalarial activity against the human malaria parasite Plasmodium falciparum. Compounds 1 and 2 exhibited the greatest cytotoxicity against all the tumor cells tested (IC50 = 5-27 microg/L) and against multidrug-resistant P. falciparum K1 strain (IC50 = 1.0-4.0 g/L) and 3 was only cytotoxic toward the leukaemia HL-60 strain (IC50 = 11.8 microg/L). Quassinoids 1 and 2 (LC50 = 3.2-4.4 mg/L) displayed greater lethality than derivative 4 (LC50 = 75.0 mg/L) toward A. aegypti larvae, while derivative 3 was inactive. These results suggest a novel application for these natural quassinoids as larvicides. The toxicity toward A. franciscana could be correlated with the activity in several biological models, a finding that is in agreement with the literature. Importantly, none of the studied compounds exhibited in vitro haemolytic activity, suggesting specificity of the observed cytotoxic effects. This study reveals the biological potential of quassinoids 1 and 2 and to a lesser extent their semi-synthetic derivatives for their in vitro antimalarial and cytotoxic activities.


Acta Amazonica | 2009

Antimicrobial activity of bergenin from Endopleura uchi (Huber) Cuatrec

Saulo L. da Silva; Viviane Guedes de Oliveira; Tomomasa Yano; Rita de Cássia Saraiva Nunomura

ABSTRACT Endopleura uchi (Huber) Cuatrec. is an Amazon species traditionally used as treatment for inflammations and female disorders. Bergenin was isolated from ethyl acetate fraction of bark of E. uchi by using column chromatography over sephadex LH-20 and then silica gel 60 flash. Its structure was identified on the basis of its NMR spectra. The antimicrobial activity of bergenin and fractions of methanol extract of E. uchi were evaluated against ATCC microorganisms ( Escherichia coli , Salmonella enteritidis , Pseudomonas aeruginosa , Enterococcus faecalis , Staphylococcus aureus , Candida albicans, C. guilliermondii , Aspergillus flavus , A. nidulans ). Clinically isolated strains of all of these microorganisms, along with C. tropicalis , A. niger , Shigella sonnei, Serratia marcenses and Klebsiella pneumoniae were also evaluated. The growth inhibition caused by bergenin, extracts and fractions of E. uchi against ATCC microorganisms were similar to the inhibition to microorganisms clinically isolated. The ethyl acetate fraction and the isolate bergenin inhibit the growth of the yeasts


Acta Amazonica | 2006

In vitro studies of the anthelmintic activity of Picrolemma sprucei Hook. f. (Simaroubaceae)

Rita de Cássia Saraiva Nunomura; Ellen Cristina Costa da Silva; Denilson Ferreira Oliveira; Adriana Mello Garcia; Jankerle Neves Boeloni; Sergio Massayoshi Nunomura; Adrian Martin Pohlit

1300 ppm (1.3 g / L), water and ethanol extracts prepared from stems or roots of Picrolemma sprucei Hook. f. were lethal (85-90 % mortality) in vitro to Haemonchus contortus (Barber Pole Worm) larvae, a gastrointestinal nematode parasite found in domestic and wild ruminants. Neosergeolide and isobrucein B were isolated in 0.0083 and 0.0070 % yield from dry, ground P. sprucei stems (0.89 kg). Neosergeolide, isobrucein B and the anthelmintic drug standard levamisole all caused comparable mortality rates (68-77 %) in vitro to H. contortus at similar concentrations (81-86 ppm). The anthelmintic activity of P. sprucei infusions (teas), alcohol extracts, and neosergeolide and isobrucein B, has therefore been demonstrated for the first time.


Revista Virtual de Química | 2016

Plantas Oleaginosas Amazônicas: Química e Atividade Antioxidante de Patauá ( Oenocarpus bataua Mart.)

Patrícia S. P. Hidalgo; Rita de Cássia Saraiva Nunomura; Sergio Massayoshi Nunomura

As especies oleaginosas amazonicas e em especial oriundas de palmeiras nativas fazem parte das paisagens e da cultura amazonica. Os oleos amazonicos possuem grande potencial na geracao de produtos medicinais, cosmeticos, nutraceuticos e na geracao de energia. A especie Oenocarpus bataua (pataua) e uma especie comestivel, a partir de seus frutos se extrai o “vinho de pataua”, que e bastante nutritivo e energetico. Frutos inteiros foram coletados nas proximidades de Manaus-AM e separados em cascas, polpa e sementes. Os extratos metanolicos da polpa e das sementes foram avaliados em diferentes ensaios de atividade antioxidante e ambos os extratos foram considerados ativos. Do extrato da polpa, foi realizado um isolamento monitorado por cromatografia em camada delgada para substâncias antioxidantes, que levou ao isolamento do estilbeno, piceatanol. Esse e o primeiro relato de isolamento de um produto natural de pataua. O piceatanol possui propriedades farmacologicas descritas, algumas inclusive superiores ao resveratrol, um antioxidante bem conhecido. Esse resultado demonstra o grande potencial do pataua na geracao de um bioproduto nutraceutico. DOI: 10.5935/1984-6835.20160009


Química Nova | 2012

Constituintes químicos dos galhos de Simaba guianensis subesp. ecaudata (Cronquist)

Rita de Cássia Saraiva Nunomura; Angelo C. Pinto; Sergio Massayoshi Nunomura; Adrian Martin Pohlit; Ana Claudia F. Amaral

Simaba guianensis subesp. ecaudata (Simaroubaceae) is a tree found in the Brazilian Amazon. This work describes for the first time the fractionation of stems of this species that resulted in the isolation of the cytotoxic triterpene piscidinol A, the alkaloid 9-methoxycanthin-6-one, caryophyllene oxide, also isolated for the first time from this species and a new alkaloid (6-methoxy-(9H-β-carbolin-1-il)-(Z)-2-propenoic acid). Quantification of 9-methoxycanthin-6-one in different extracts and fractions of stems of S. guianensis by high performance liquid chromatography was also performed. The concentration of 9-methoxycanthin-6-one in methanolic and aqueous extracts were inferior to the known cytotoxic concentration of this compound.


Acta Amazonica | 2014

Constituintes químicos isolados dos galhos e cascas de amapazeiro (Parahancornia amapa, Apocynaceae)

Marycleuma Campos Henrique; Rita de Cássia Saraiva Nunomura; Sergio Massayoshi Nunomura; Suniá Gomes Silva

Parahancornia amapa (Apocynaceae) is typical of the Amazon region and popularly known as amapazeiro and widely used in folk medicine in the Amazon region. The objective of this work was to study the phytochemical composition of twigs and barks of this species. From the dichloromethane extract of twigs were isolated β-sitosterol, stigmasterol, and pentacyclic triterpenoids α-amyrin, β-amyrin, lupeol and friedelin. From the methanol extract of barks was isolated indole alkaloid isositsiriquina. The structures of these compounds were identified by analysis of the mass spectra high-resolution, 1H and 13C NMR and comparisons with literature data. This is the first report of isolation of alkaloid in this genus.


Química Nova | 2012

Limonoides isolados dos frutos de Carapa guianensis Aublet (Meliaceae)

Suniá Gomes Silva; Rita de Cássia Saraiva Nunomura; Sergio Massayoshi Nunomura

Six limonoids were isolated in hexane extract obtained from the seeds and pericarps of Carapa guianensis. The structures of the limonoids were determined based on the analysis of High Resolution Mass Spectroscopy and Nuclear Magnetic Resonance (uni-and bi-dimensional experiments) data. This is the first report of isolation of the limonoid 6α-acetoxy-7-deacetilgedunin from the seeds of the C. guianensis species. The limonoid 6-hydroxy-methyl angolensate was also described for the first time in this species.


Food Chemistry | 2018

Non-thermal combined treatments in the processing of açai (Euterpe oleracea) juice

Ana Flávia A. Oliveira; Josiana M. Mar; Samara F. Santos; Joel L. da Silva Júnior; Ariane M. Kluczkovski; Amr M. Bakry; Jaqueline de A. Bezerra; Rita de Cássia Saraiva Nunomura; Edgar A. Sanches; Pedro Henrique Campelo

Quality parameters of açai juice processed with ultrasound-assisted, ozone and the combined methods were analyzed in this work. Two ultrasound energy densities (350 and 700 J·mL-1) and two ozonization times (5 and 10 min with 1.5 ppm) were analyzed for pure açai juice and 8 different treatments (22 complete factorial). To evaluate the quality parameters of the juice, physical-chemical analyzes such as pH, titratable acidity, cloud value, non-enzymatic browning, rheology, antioxidant activity (DPPH and ABTS), phenolic compounds, anthocyanins, enzymatic activity (peroxidase and polyphenol oxidase) and microbial counts (mesophilic bacteria, molds and yeasts) were conducted. The treatments with ozone were better for microbial inactivation and the ultrasound for enzymatic inactivation. In general, the use of non-thermal methods can be a good alternative for the processing of açai juice.

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Afonso D. L. de Souza

Federal University of Amazonas

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Edgar A. Sanches

Federal University of Amazonas

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Emerson Silva Lima

Federal University of Amazonas

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Felipe M.A. da Silva

Federal University of Amazonas

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Giovana A. Bataglion

Federal University of Amazonas

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