Robert Chernecky

The effect of various storage methods and media on shear-bond strengths of dental composite resin to bovine dentine

A variety of media and methods have been used to store teeth used in bond-strength studies of resin restorative materials to dentine. This study examined the effect of 2 months of storage using 11 different methods and media on the shear-bond strength of Z100 resin composite to bovine dentine mediated by Scotchbond Multi Purpose adhesive. Freshly harvested teeth were used as controls. The results showed that 7 of the l1 storage methods or media were similar in shear-bond strengths and bond-failure characteristics. Four of the 11 methods (irradiation, or storage in thymol, methanol, and glutaraldehyde) resulted in significantly lower shear-bond strengths and atypical shear-bond failure, indicating that these are the least desirable methods of tooth storage for studies of this type. This study has further shown that if insufficient numbers of fresh teeth are available for studies of shear-bond strength, then freezing is the preferred method of storage for the registration of high shear-bond strengths. It is also apparent that further investigations are needed to examine what post mortem changes occur in dentine, whether these changes are modified by various storage conditions, and whether they have any significant effect on bonding of resin composites.

Biaxial mechanical/structural effects of equibiaxial strain during crosslinking of bovine pericardial xenograft materials.

We have investigated the effect of biaxial constraint during glutaraldehyde crosslinking on the equibiaxial mechanical properties of bovine pericardium. Crosslinking of cruciate samples was carried out with: (i) no applied load, (ii) an initial 25 g ( approximately 30 kPa) equibiaxial load, or (iii) an initial 200 g (approximately 250 kPa) equibiaxial load. All loading during crosslinking was done under a defined initial equibiaxial load and subsequently fixed biaxial strain. Load changes during crosslinking were monitored. Mechanical testing and constraint during crosslinking were carried out in a custom-built biaxial servo-hydraulic testing system incorporating four actuators with phase-controlled waveform synthesis, high frame-rate video dimension analysis, and computer-interfaced data acquisition. The paired biaxial stress strain responses under equibiaxial loading at 1 Hz (before and after treatment) were evaluated for changes in anisotropic extensibility by calculation of an anisotropy index. Scanning electron microscopy (SEM) was performed on freeze-fractured samples to relate collagen crimp morphology to constraint during crosslinking. Fresh tissue was markedly anisotropic with the base-to-apex direction of the pericardium being less extensible and stiffer than the circumferential direction. After unconstrained crosslinking, the extensibility in the circumferential direction, the stiffness in the base-to-apex direction, and the tissues anisotropy were all reduced. Anisotropy was preserved in the tissue treated with an applied 25 g load; however, tissue treated with an applied 200 g load became extremely stiff and nearly isotropic. SEM micrographs correlated well with observed extensibility in that the collagen fibre morphology changed from very crimped (unconstrained crosslinking), to straight (200 g applied load). Biaxial stress-fixation may allow engineering of bioprosthetic materials for specific medical applications.

SEM observations of the reactions of the components of a light-activated glass polyalkenoate (ionomer) cement on bovine dentine

OBJECTIVES Glass ionomer cements are used in clinical dentistry as lining, luting and restorative materials. The precise nature of their bonding mechanism to dentine is unclear. This study is an SEM examination of the effect of the liquid contained in Vitrebond cement (3M Co., MN) on the surface of bovine dentine, with respect to delays in photocuring and washing off with water under pressure or as a gentle stream. The effects of delaying photocuring of the mixed and applied cement for up to 120 s were also examined. RESULTS The results of this study demonstrated that the liquid component of Vitrebond reacts chemically with dentine in a manner suggestive of an effervescent chemical reaction. This reaction produces plugs in the dentinal tubules which are resistant to dislodgement by water under pressure or by gentle washing. Similarly, a delay in photocuring the mixed and applied cements results in porosity of the cement at the dentine interface. CONCLUSION The findings in this study suggest that the adhesion of Vitrebond to dentine is primarily chemical in nature and that its mechanical strength is compromised if there are substantial delays in photocuring.

Titanemia from total knee arthroplasty: A case resulting from a failed patellar component

The subject of this case report is a patient with elevated serum levels of titanium (77 parts/billion [ppb]; normal, 3.3 ppb) and vanadium (0.38 ppb; normal, 0.17 ppb) resulting from excessive wear of a metal-backed patellar component in a total knee arthroplasty. The patellar component was worn through both its polyethylene and metal backing as a result of abnormal contact between the patellar and femoral components. Scanning electron microscopic examination of the ingrowth surface of the patellar component indicated that particle debonding occurred as a result of overloading of the sintered neck regions at the particle-substrate interface, suggesting a possible damage during initial insertion of the device, which may have predisposed it to loosening and abnormal contact with the femoral component. Wear particles resulted in staining of the tissues within the knee and an inflammatory and immune response in the synovium consisting of giant cells and T lymphocytes. The serum metal levels were reduced 22 weeks after replacing the patellar component; however, the titanium level was still slightly elevated (8 ppb).

Bone Interfaces with Retrieved Alumina and Hydroxyapatite Ceramics

ABSTRACT Single crystal alumina, polycrystalline alumina and dense hydroxyapatite ceramic disc implants were retrieved after implantation in rabbit mandibular bone. Implants were examined by scanning electron microscopy. Polycrystalline alumina and dense hydroxyapatite showed the presence of a calcium phosphate extracellular matrix juxtaposed to their surfaces. In the latter two distinctly different interfacial morphologies were observed; either close bone apposition or bonding. The results suggest that the micro-topography of an implant influences the tissue type at the interface and that bonding to dense hydroxyapatite ceramics may be due to a micro-mechanical interlocking of calcified extracellular matrix with intergranular microvoids in the ceramic surface.

Effect of Two Antibacterial Mouth Sprays and Dentifrices on Dental Plaque and Gingivitis in Beagle Dogs

Plaque inhibition by dilute mouth sprays and dentifrices containing benzethonium chloride and chlorhexidine gluconate was compared in beagle dogs. Agents with chlorhexidine gluconate produced less plaque than their benzethonium chloride or placebo counterparts, but the differences were not significant when compared to mean control scores registered during interexperimental recovery periods.