Robert E. Jones

An immunohistochemical study of dermatofibrosarcoma protuberans supports its fibroblastic character and contradicts neuroectodermal or histiocytic components

Paraffin-embedded material from 26 cases of dermatofibrosarcoma protuberans (DFSP) was investigated by the peroxidase-antiperoxidase technique. Antibodies to S-100 protein, Leu-7 antigen, and neuron-specific enolase (neural markers); to lysozyme,

Histological and immunohistological identification of collagens in basement membranes of Schwann cells of neurofibromas.

aP-1-antitrypsin, and

Solitary Keratoacanthoma Is a Squamous-cell Carcinoma: Three Examples with Metastases

aP-1-antichymotrypsin (histiocytic markers); and to cytokeratin, desmin, vimentin, and factor VIII were used. The tumor cells reacted only for vimentin. In addition, 12 cases showed positive reactions with histiocytic markers (1–3% of the cells; in two cases, up to 10%). These results support a fibroblastic and contradict a neural or histiocytic histogenesis of DFSP.

Mammary Paget's disease without underlying carcinoma

The application of immunohistological techniques that use antibodies highly specific for collagen allows new comparative studies in the distribution and deposition of genetically distinct types of collagens in neurofibromas. The identification of collagen type IV in basement membranes is unique and marks particularly the sheaths or cytoplasms of proliferating Schwann cells. Cells with greater degrees of atypia show increased amounts of intracellular collagen in basement membranes by immunohistologic examination, which provides an additional diagnostic tool in dermatopathology.