Robert L. Barnett

Testate amoebae as sea-level indicators in Northwestern Norway: developments in sample preparation and analysis

Proxy based sea-level reconstructions are an important tool for defining past patterns of sea-level change and salt-marsh testate amoebae are a newly emerging proxy with high potential as sea-level indicators. This study develops existing analytical techniques con- cerned with the preparation and counting of testate amoebae for sea-level studies and demonstrates the predictive power of this group of micro-organisms. Two salt marshes in northwestern Norway were sampled for testate amoebae and multiple sub-samples were prepared using different procedures and count totals. Analytical efficiency can be improved upon by using a mild alkali, chemical disaggregant (5% KOH) to break up fibrous, salt-marsh peat and concentrate tests prior to counting. A count total of 100 individuals, rather than 150, can be used to make time gains with little or no loss of taxon information. The Norwegian salt-marsh testate amoebae showed strong zonation relative to tidal elevation. Key indicator taxa from the high marsh included Centropyxis cassis type, Cyclopyxis arcelloides type and Eugly- pha spp. Those from the low marsh included Difflugia pristis type and a distinctive morphotype of Centropyxis platystoma type. Combined, the two surface data sets from Norway were capable of predicting marsh surface elevations to within ± 0.09 m.

The legacy of 4,500 years of polyculture agroforestry in the eastern Amazon

The legacy of pre-Columbian land use in the Amazonian rainforest is one of the most controversial topics in the social1–10 and natural sciences11,12. Until now, the debate has been limited to discipline-specific studies, based purely on archaeological data8, modern vegetation13, modern ethnographic data3 or a limited integration of archaeological and palaeoecological data12. The lack of integrated studies to connect past land use with modern vegetation has left questions about the legacy of pre-Columbian land use on the modern vegetation composition in the Amazon, unanswered11. Here, we show that persistent anthropogenic landscapes for the past 4,500 years have had an enduring legacy on the hyperdominance of edible plants in modern forests in the eastern Amazon. We found an abrupt enrichment of edible plant species in fossil lake and terrestrial records associated with pre-Columbian occupation. Our results demonstrate that, through closed-canopy forest enrichment, limited clearing for crop cultivation and low-severity fire management, long-term food security was attained despite climate and social changes. Our results suggest that, in the eastern Amazon, the subsistence basis for the development of complex societies began ~4,500 years ago with the adoption of polyculture agroforestry, combining the cultivation of multiple annual crops with the progressive enrichment of edible forest species and the exploitation of aquatic resources. This subsistence strategy intensified with the later development of Amazonian dark earths, enabling the expansion of maize cultivation to the Belterra Plateau, providing a food production system that sustained growing human populations in the eastern Amazon. Furthermore, these millennial-scale polyculture agroforestry systems have an enduring legacy on the hyperdominance of edible plants in modern forests in the eastern Amazon. Together, our data provide a long-term example of past anthropogenic land use that can inform management and conservation efforts in modern Amazonian ecosystems.Fossil records suggest that the Amazon rainforest in the pre-Columbian era was home to polyculture agroforestry, with multiple annual crops providing subsistence for indigenous groups who shaped the Amazon as early as 4,500 years ago.

Distribution of Salt-Marsh Foraminifera in Two South African Estuaries and the Application as Sea-Level Indicators

ABSTRACT Strachan, K.L.; Hill, T.R.; Finch, J.M.; Barnett, R.L., and Frenzel, P., 2017. Distribution of salt-marsh foraminifera in two South African estuaries and the application as sea-level indicators. The global mean sea level is rising as a result of climate change and is likely to affect millions of people. It is essential to understand and quantify regional relative sea-level variability to be able to predict future changes. Proxy evidence is necessary for extending our understanding of past sea-level changes beyond the industrial era, and salt-marsh foraminifera have become an important tool for reconstructing late Holocene sea-level changes. In South Africa, little is known regarding the distribution of salt-marsh foraminifera and their use as sea-level indicators, thereby limiting their application in sea-level research. This study therefore describes the distribution of living and dead surface foraminifera from two study sites along the SE South African coastline. The full surface dataset has been compiled from 139 samples that are used to describe the contemporary distribution of salt-marsh foraminifera. Cluster analysis is used to define four biozones; high marsh, middle marsh, low marsh, and mudflats. In the high marsh, where environmental conditions reach the survival threshold, a greater abundance of agglutinated foraminifera occurs. In the low-marsh zone, where subaerial exposure is restricted and environmental conditions are usually stable, a greater diversity of calcareous species occurs. The tidal mudflats have the highest diversity of calcareous assemblages with some agglutinated taxa present. Distributions of living foraminiferal populations are similar to the population distributions of dead foraminifera at both sites in the low-marsh and mudflat zones. In the high-marsh zones, however, the living-to-dead ratio and distributions are different, which could be a result of different influences of environmental variables along with seasonal variations. This study provides insights into foraminiferal distributions along the SE coastline of South Africa, which will be useful for interpreting late Holocene sea-level changes.