Robert M. Pike

Production of 7S and 19S Antibodies to the Somatic Antigens of Salmonella typhosa in Rabbits.

Summary Seven rabbits immunized with acetone dried Salmonella typhosa produced both 19S and 7S agglutinins for the typhoid somatic antigens although the 19S antibodies predominated in most serum samples even after repeated injections of vaccine. The results suggest that the route of injection and amount of antigen injected may influence the relative amounts of 19S and 7S antibodies appearing in the serum.

Chromatographic Analysis and Sulfhydryl Sensitivity of Antileptospira Agglutinins in Rabbit and Human Sera.

Summary Chromatography on DEAE cellulose and susceptibility to inactivation by 2-mercaptoethanol indicate that the major portion of the agglutinins in patients”sera for 7 types of leptospirae are 19S macroglobulins even at 4 to 8 weeks following the onset of symptoms. A distinctly greater proportion of the agglutinins for L. sejroe was found in the 7S fraction of patients”sera. The agglutinins produced in rabbits following the injection of L. pomona and L. sejroe were initially almost entirely in the 19S fraction of serum but appeared also in the 7S fraction as time progressed. L. sejroe showed a greater tendency to elicit the production of 7S agglutinins in the rabbit than did L. pomona.