Robert Neurath

Radioimmunoassay for Albumin-Binding Sites Associated with HBsAg: Correlation of Results with the Presence of e-Antigen in Serum

A radioimmunoassay for albumin-binding sites associated with hepatitis B surface antigen (HBsAg) is described. Polystyrene beads coated with glutaraldehyde-polymerized human serum albumin were incubated with diluted specimens of HBsAg-positive sera which contained either e-antigen (HBeAg) or anti-HBe or were negative for both HBeAg and anti-HBe as determined by rheophoresis. The quantity of HBsAg attached to the beads, determined by measuring the amount of 125I-labeled anti-HBs adsorbed during a subsequent incubation step, was related to the presence of HBeAg in the specimens.

A New Reversed Passive Hemagglutination Test for Detection of HBsAg

Abstract. A new reversed passive hemagglutination test for HBsAg, termed Raphadex B, has been developed using immunochemically purified chimpanzee anti‐HBs bound to stabilized human erythrocytes. The test has been found to have equivalent sensitivity to the Ausria 125I radioimmunoassay, and detected a similar number of HBsAg‐containing specimens in screening of volunteer blood donors. This method offers an economical approach to third generation methodology for hepatitis B screening of blood donors.