Robert S. Vernon

Wireworm Management I: Stand Protection Versus Wireworm Mortality With Wheat Seed Treatments

ABSTRACT The efficacy of various insecticidal seed treatments in protecting wheat, Triticum aestivum L., from wireworm damage as well as reducing wireworm (Coleoptera: Elateridae) populations was studied over 3 yr. Protection from wireworm damage was measured by postplanting stand counts, and effects on wireworm populations were measured by within-row core samples and by bait traps placed in plots the following spring. The effects of treatments on populations of larger wireworms already present at planting were distinguished from their effects on neonate wireworms produced that growing season. Neonicotinoid seed treatments (imidacloprid, clothianidin, and thiamethoxam) provided excellent wheat stand protection, likely through prolonged wireworm intoxication, but populations of larger and neonate wireworms were not significantly reduced in bait traps the following spring. The pyrethroid tefluthrin, applied to seed with and without a neonicotinoid insecticide (thiamethoxam), provided excellent crop protection, but populations of wireworms also were not significantly reduced. This and additional laboratory data suggest that wheat stand establishment provided by tefluthrin is due to a combination of repulsion and short term morbidity events. The phenyl pyrazole fipronil provided excellent crop protection, and populations of both larger and neonate wireworms could not be detected in plots the following spring. The previously registered organochlorine lindane, although reducing wireworm feeding, was phytotoxic in 2 of 3 yr. Next to fipronil, lindane was the most consistent seed treatment in reducing populations of larger and neonate wireworms. These studies indicate that stand and yield protection provided by contemporary wheat seed treatments cannot automatically be equated with wireworm population mortality. This is an important consideration when choosing a suitable seed treatment to replace lindane, which historically provided both stand protection and wireworm reduction, and did not have to be applied every year.

Biology, Ecology, and Control of Elaterid Beetles in Agricultural Land*

Wireworms, the larvae of click beetles (Coleoptera: Elateridae), have had a centuries-long role as major soil insect pests worldwide. With insecticidal control options dwindling, research on click beetle biology and ecology is of increasing importance in the development of new control tactics. Methodological improvements have deepened our understanding of how larvae and adults spatially and temporarily utilize agricultural habitats and interact with their environment. This progress, however, rests with a few pest species, and efforts to obtain comparable knowledge on other economically important elaterids are crucial. There are still considerable gaps in our understanding of female and larval ecology; movement of elaterids within landscapes; and the impact of natural enemies, cultivation practices, and environmental change on elaterid population dynamics. This knowledge will allow generation of multifaceted control strategies, including cultural, physical, and chemical measures, tailored toward species complexes and crops across a range of appropriate spatial scales.

Soil Bioassay for Studying Behavioral Responses of Wireworms (Coleoptera: Elateridae) to Insecticide-Treated Wheat Seed

Abstract A bioassay for observing wireworm behavior in soil is described. The bioassay permits analysis of orientation, feeding, repellency, and postcontact toxicity behaviors of wireworms in response to insecticide-treated wheat seeds. Wireworm positions were recorded every 5 min for 3 h, and the time required to orient to and contact seeds, and the duration of individual feeding events, was calculated. Both avoidance (before contact with seeds) and repellency (after contact) were quantified. A high proportion of Agriotes obscurus (0.95), Limonius canus (1.00), Ctenicera pruinina (0.80), Melanotus communis/dietrichi (0.80), and Hypolithus sp. (0.70) larvae contacted untreated wheat seeds and began feeding within 120 min when seeds were preincubated for 60 min in soil with 20% moisture. A smaller proportion of A. obscurus contacted seeds if seeds were not incubated in the bioassay before wireworm introduction (0.80) or in soil with 10% moisture (0.65). L. canus larvae required a significantly shorter time (25.3 min) to contact seeds if seeds were incubated for 60 min than if seeds were not incubated before wireworm introduction (43.1 min). Wireworms exposed to untreated seeds and seeds treated with the fungicide Dividend XLRTA fed normally (i.e., sustained feeding for at least 60 min), but a significant proportion of wireworms exposed to seeds treated with Tefluthrin 20 CS (containing the synthetic pyrethroid tefluthrin) fed for 15 min or less and were subsequently repelled. Wireworms exposed to Vitavax Dual (containing the organochlorine lindane) were not repelled after feeding and showed symptoms of illness for up to 28 d before making a full recovery (89%) or dying (11%).

Wireworms as Pests of Potato

Wireworms (Coleoptera: Elateridae) of several species cause severe cosmetic and economic injury to daughter tubers. Populations are expanding globally, due largely to the attrition of most organochlorine and organophosphate insecticides used since the 1950s. Where wireworms could once be controlled for several years with organochlorine insecticides, contemporary management strategies for wireworms are taking on more regional, species-specific and multi-tactical approaches. The requisite knowledge and expertise underlying these approaches involves taxonomy (traditional and molecular); biology and ecology (life history, behavior, distribution, habitat and host preferences, chemical ecology, phenology); surveillance (wireworm and adult sampling and risk assessment); and species-specific response(s) to a variety of integrated pest management approaches (e.g. crop rotation, cultivation, tolerant varieties, soil amendments, biological and semiochemical controls and low-risk insecticides). These topics are reviewed in this chapter, along with suggestions as to the more likely and productive avenues of research to address now and in the future.

Importance of Collection Overhangs on the Efficacy of Exclusion Fences for Managing Cabbage Flies (Diptera: Anthomyiidae)

Abstract Fine nylon mesh fences (135 cm high) with varying lengths of downward-sloping collection overhangs were evaluated for efficacy in excluding the female cabbage flies Delia radicum (L.) from plots of radish, Raphanus sativus (L.). During three trials conducted in 1994 and 1995, fences without overhangs, fences with 12.5-cm overhangs, or fences with 50-cm overhangs were tested against fences with standard 25-cm overhangs and unfenced control plots. In fenced plots with standard 25-cm overhangs, the mean number of D. radicum females caught on yellow sticky traps placed within plots was 85% less than those caught in corresponding control plots. The mean numbers of D. radicum females caught in fenced enclosures with no overhangs, 12.5-cm overhangs, or 50-cm overhangs, were 61, 67, and 94% less than those caught in corresponding control plots, respectively. The mean proportion of radishes damaged by D. radicum larvae inside enclosures with 25-cm overhangs was 62% less than in corresponding control plots. The mean proportions of radishes damaged inside fences with no overhangs, 12.5-cm overhangs, or 50-cm overhangs were 33, 59, and 81% less than those caught in corresponding control plots, respectively. Results are discussed in terms of defining an appropriate fence design for commercial use.

Morbidity and recovery of the Pacific Coast wireworm, Limonius canus, following contact with tefluthrin-treated wheat seeds

Late instars of the Pacific Coast wireworm, Limonius canus (LeConte) (Coleoptera: Elateridae), were exposed to wheat seeds treated with tefluthrin at 5, 10, 15, 20, or 30 g active ingredient (a.i.) per 100 kg wheat seed for 1, 2, 4, 8, or 16 min. All wireworms were moribund within 20 min of first exposure and recovered fully within 12 h. The time required for recovery (tr) after a single exposure increased with duration of exposure (e) and concentration (c), but decreased with wireworm weight (w), expressed as (tr)0.5 = 5.2812 + 0.9407e – 0.0259e2 + 0.1569c + 0.0254ec – 0.0174w – 0.0057ew. For wireworms exposed to treated seeds for 2 min, the time required for induction of morbidity decreased as concentration of tefluthrin increased and as wireworm weight decreased, expressed as (ti)0.5 = 2.613 – 0.039c + 0.018w, where ti is the induction time of morbidity, and c and w are as above. Wireworms re‐exposed to tefluthrin‐treated seeds after recovery from previous exposure were again moribund within 20 min of exposure, but recovery was significantly more rapid if the second exposure was 2, 4, 6, 8, 12, 18, 24, and 48 h after recovery from first exposure. Recovery from a second exposure was not significantly faster when the second exposure was 96 h after recovery from the first exposure. The ability of wireworms to recover from tefluthrin‐induced morbidity may seriously limit the efficacy of this insecticide in actually reducing wireworm populations in the field.

Further Studies on Wireworm Management in Canada: Damage Protection Versus Wireworm Mortality in Potatoes

ABSTRACT The efficacy of various insecticides and application methods in protecting potatoes from wireworm (Agriotes obscurus L.) damage as well as reducing wireworm populations was studied over 5 yr in Agassiz, British Columbia. Protection from wireworm damage was measured by the number of blemishes to daughter tubers, and effects on wireworm populations were measured by sampling soil around seed potatoes and/or by bait traps the following spring. Organophosphates registered for wireworm control in the United States and/or Canada (phorate and chlorpyrifos), significantly reduced blemishes to tubers by, respectively, 92.2 and 90.2%, and populations of large (≥9 mm long = ‘resident’) wireworms by 83.4 and 71.0% relative to controls. Similar reductions in smaller (<9 mm long = ‘neonate‘) wireworms were also observed. Neonicotinoids (imidacloprid, clothianidin, and thiamethoxam) tested as seed piece treatments at ≈12.5 g active ingredient/100 kg potato seed reduced blemishes by, respectively, 19.1, 71.6, and 90.6%, but resident wireworms were only reduced by, respectively, 22.0, 29.1, and 51.8%. Significant mortality of neonates was not observed with any neonicotinoid treatment. With the possible exception of thiamethoxam, where significant reduction in resident wireworms occurred, it is likely that the blemish protection provided without significant wireworm mortality with imidacloprid and clothianidin treatments was because of long-term wireworm intoxication followed by population recovery. The phenyl pyrazole, fipronil, applied as an in-furrow spray reduced blemishes by 94.3%, and resident wireworm populations could not be detected in plots the following spring. Neonates were reduced by 93.3%, indicating excellent residual control had occurred with fipronil. These studies indicate that tuber protection by fipronil and currently registered organophosphates is likely because of significant early season mortality of wireworms, whereas neonicotinoids generally provide control through long-term morbidity without high levels of mortality occurring.

Portable Trench Barrier for Protecting Edges of Tomato Fields from Colorado Potato Beetle (Coleoptera: Chrysomelidae)

Abstract Experiments were conducted to test a portable trench barrier composed of an extruded, UV-retarded, PVC plastic trough, designed to allow Colorado potato beetles, Leptinotarsa decemlineata (Say), to enter and become trapped and killed inside. Tests demonstrated that the portable plastic trenches were effective as barriers to Colorado potato beetles as they walked into tomato, Lycopersicon esculentum Mill., fields from overwintering sites in the spring. In field tests, plots that were protected by portable trench barriers had significantly fewer beetles per tomato plant, and lower levels of defoliation. Tomato yields in plots that were protected by portable trench barriers were similar to yields in plots that were protected by insecticide sprays, and significantly higher than plots where beetles were not controlled.

Root Weevil (Coleoptera: Curculionidae) and Ground Beetle (Coleoptera: Carabidae) Immigration into Strawberry Plots Protected by Fence or Portable Trench Barriers

Abstract Physical exclusion shows some potential as a novel root weevil control strategy, but barriers to root weevil immigration may also exclude beneficial insects, such as ground beetles. A field study was undertaken in 1997 to assess the impact of two physical barriers—portable plastic trenches and aluminum fences with Teflon tape—on root weevil and ground beetle immigration into plots of strawberry, Fragaria x ananassa (Duchesne). Barypeithes pellucidus (Boheman) and Nemocestes incomptus (Horn), each comprised 43% of the root weevils caught at the site. Most (86%) of the ground beetles caught in control plots were longer than 1 cm, the width of the gap in the portable trench top. Trenches excluded 75 and 63% of B. pellucidus and N. incomptus, respectively, without significantly reducing immigration of large (<1 cm) ground beetles. Fences excluded 65, 84, and 99% of B. pellucidus, N. incomptus, and large ground beetles, respectively. Adding diatomaceous earth to trenches did not increase their efficacy, and fences without Teflon tape excluded ground beetles but not root weevils. The reduction in the population of root weevils and other strawberry pests caused by the use of barriers reduced damage to strawberry plant leaves and increased strawberry plant survival relative to unprotected control plots. Advantages and disadvantages of these physical control tools are discussed with a view to creating superior tools for root weevil exclusion, compatible with an integrated pest management approach. Portable trenches may offer a means of selectively excluding root weevils but not ground beetles.

Effect of Crop Rotation on Populations of Epitrix tuberis (Coleoptera: Chrysomelidae) in Potato

Abstract The effect of crop rotation on populations of tuber flea beetle, Epitrix tuberis Gentner, in potatoes was investigated using data supplied by an integrated pest management (IPM) company and Geographic Information System software and conventional statistical methods. Using combined 1995 and 1996 data, beetles of the overwintered and F1 generations in both the interior and edges of potato fields showed a significant linear increase with an increase in the preceding consecutive years (0, 1, and 2 years) that the current years’ crop was planted to potatoes. Populations were significantly higher in nonrotated fields compared with rotated fields. Both the percentage of the cropping region requiring insecticidal control of tuber flea beetles and the cost of insecticides per hectare of potatoes grown increased linearly with an increase in the number of previous years planted to potatoes. Not practicing crop rotation resulted in a 4.2–7.3% increase in the cropping region requiring insecticidal control of tuber flea beetles. The cost of controlling beetles in potato fields planted to potatoes for 3 consecutive years was up to