Roberto Sergio Azzoni

Light-dependent microbial metabolisms drive carbon fluxes on glacier surfaces.

Biological processes on glacier surfaces affect glacier reflectance, influence surface energy budget and glacier response to climate warming, and determine glacier carbon exchange with the atmosphere. Currently, carbon balance of supraglacial environment is assessed as the balance between the activity of oxygenic phototrophs and the respiration rate of heterotrophic organisms. Here we present a metagenomic analysis of tiny wind-blown supraglacial sediment (cryoconite) from Baltoro (Pakistani Karakoram) and Forni (Italian Alps) glaciers, providing evidence for the occurrence in these environments of different and previously neglected metabolic pathways. Indeed, we observed high abundance of heterotrophic anoxygenic phototrophs, suggesting that light might directly supplement the energy demand of some bacterial strains allowing them to use as carbon source organic molecules, which otherwise would be respired. Furthermore, data suggest that CO2 could be produced also by microbiologically mediated oxidation of CO, which may be produced by photodegradation of organic matter.

Spinal cord sonography in newborns: Anatomy and diseases

Sonography of the spinal cord is a relatively recent acquisition that makes it possible to evaluate the content of the vertebral canal and study its pathologies. The aim of this study was to verify the reliability of ultrasound images by comparing them with magnetic resonance ones in healthy controls as well as in patients referred to us between 1991 and 2004. In this period, we studied 436 newborns: 88 without any suspicion of disease as normal controls, and 348 with suspected congenital diseases or in order to screen the children of diabetic mothers, a group that has shown an increased incidence of dysraphism. After explaining normal sonograms, we describe the pathological pictures observed in the 12 pathological cases in our series: conus hypomobility in five cases; lack of visualization of the conus medullaris in one case; and an enlarged ependymal canal in six cases. Four cases presented all three pathological conditions, and seven the association of two pathologies. All of these patients also underwent magnetic resonance imaging (MRI), which confirmed the ultrasound findings in four cases: three cases of enlarged ependymal canal and one of tethered cord hypomobility of the roots with an associated lipoma; the MRI findings were normal in the other seven cases. Sonography was highly specific but not very sensitive, because it is partially conditioned by patient collaboration. Nevertheless, subsequent MRI confirmed 37% of the suspected pathological cases. The ultrasound resolution of both normal and pathological spinal cord structures was particularly clear. The images were similar, easily comparable and often identical to the MRI results, although MRI was certainly more sensitive. The advantages of sonography are its non-invasiveness, low cost, the virtually ubiquitous availability of ultrasound equipment, the simplicity and rapidity of the examination, and its specificity. We believe that the indications for its use are lumbo-sacral skin alterations, neurological disorders caused by congenital malformations, traumas due to childbirth or a lumbar puncture, occult dysraphism, all of the compressive spinal cord neo-formations involving nerves, the dura mater and vertebral bone and joint structures, and the screening of the newborns of diabetic mothers.

Nematodes and rotifers on two Alpine debris-covered glaciers

Abstract Debris-covered glaciers (DCGs) are glaciers whose ablation area is mostly covered by a continuous layer of debris, and are considered to be among the continental glacierized environments richest in life. DCG colonization by microorganisms, plants and animals, has been investigated in a few studies, while the meiofauna (metazoans smaller than 2 mm) of these environments has been neglected so far. In this study, we analyzed nematode and rotifer fauna on the two largest debris-covered glaciers of the Italian Alps: the Miage Glacier and the Belvedere Glacier. In total, we collected 38 debris samples on the glaciers in July and September 2009. All the rotifers we found belonged to the bdelloid Adineta vaga (Davis, 1873). Nematodes belonged to 19 species. Miage Glacier hosted a richer and more diverse nematode fauna than the Belvedere. The dominant genus was Plectus Bastian, 1865, a common genus in habitats at high latitude and altitude. Analysis of the feeding type of nematodes highlighted that bacterivores were dominant on Miage Glacier, while bacterivores and herbivores were more widespread on Belvedere Glacier. Predator nematodes were absent. Analysis of the food-web structure indicated that nematode assemblages on both glaciers were typical of environments with depleted food availability, probably resulting from instability of the glacier surface and the short exposure of sediments, preventing the evolution of true soil and enrichment in organic matter of the debris. The scarcity of bacterial primary producers suggests that deposition of allochthonous organic matter is the principal organic carbon source in this environment.

Dysplasia epiphysealis hemimelica of the talus.

Dysplasia epiphysealis hemimelica is a rare developmental disorder with unknown etiology affecting epiphysis in childhood. The lesion is an osteochondroma arising from the epiphysis and increasing in size until skeletal maturity is reached. Surgical treatment is mandatory when symptoms such as pain, joint impingement or deformation are present, and yields good results when the mass is juxtaarticular or extraarticular. In those cases where articular symptoms are not present and only mass evolution is observed, surgical treatment is not recommended before skeletal maturity has been reached. A case of DEH located in the talus and successfully treated with surgery is presented.

Temporal variability of bacterial communities in cryoconite on an alpine glacier

Cryoconite holes, that is, small ponds that form on glacier surface, are considered the most biologically active environments on glaciers. Bacterial communities in these environments have been extensively studied, but often through snapshot studies based on the assumption of a general stability of community structure. In this study, the temporal variation of bacterial communities in cryoconite holes on the Forni Glacier (Italian Alps) was investigated by high throughput DNA sequencing. A temporal change of bacterial communities was observed with autotrophic Cyanobacteria populations dominating communities after snowmelt, and heterotrophic Sphingobacteriales populations increasing in abundance later in the season. Bacterial communities also varied according to hole depth and area, amount of organic matter in the cryoconite and oxygen concentration. However, variation in environmental features explained a lower fraction of the variation in bacterial communities than temporal variation. Temporal change along ablation season seems therefore more important than local environmental conditions in shaping bacterial communities of cryoconite of the Forni Glacier. These findings challenge the assumption that bacterial communities of cryoconite holes are stable.

Potential sources of bacteria colonizing the cryoconite of an Alpine glacier

We investigated the potential contribution of ice-marginal environments to the microbial communities of cryoconite holes, small depressions filled with meltwater that form on the surface of Forni Glacier (Italian Alps). Cryoconite holes are considered the most biologically active environments on glaciers. Bacteria can colonize these environments by short-range transport from ice-marginal environments or by long-range transport from distant areas. We used high throughput DNA sequencing to identify Operational Taxonomic Units (OTUs) present in cryoconite holes and three ice-marginal environments, the moraines, the glacier forefield, and a large (> 3 m high) ice-cored dirt cone occurring on the glacier surface. Bacterial communities of cryoconite holes were different from those of ice-marginal environments and hosted fewer OTUs. However, a network analysis revealed that the cryoconite holes shared more OTUs with the moraines and the dirt cone than with the glacier forefield. Ice-marginal environments may therefore act as sources of bacteria for cryoconite holes, but differences in environmental conditions limit the number of bacterial strains that may survive in them. At the same time, cryoconite holes host a few OTUs that were not found in any ice-marginal environment we sampled, thus suggesting that some bacterial populations are positively selected by the specific environmental conditions of the cryoconite holes.

Recent structural evolution of Forni Glacier tongue (Ortles-Cevedale Group, Central Italian Alps)

ABSTRACT Structural glaciology yields important details about the evolution of glacier dynamics in response to climate change. The maps provided here document the occurrence and evolution of brittle and ductile structures on the tongue of Forni Glacier, Ortles-Cevedale Group, Central Italian Alps, between 2003 and 2014. Through the remote sensing-based analysis of structures, we found evidence of brittle fractures such as crevasses, faults and ring faults, and ductile structures such as ogives at the base of the icefall in the eastern glacier tongue. Although each of the three glacier tongues have evolved differently, a reduction in flow-related dynamics and an increase in the number of collapse structures occurred over the study period. Analysis of the glacier structural evolution based on the numbers and the locations of different structures, suggest a slowdown of glacier flow on the eastern tongue. The recent evolution of the glacier also suggests that the occurrence of a disintegration scenario is likely to worsen over the next decades.

Geomorphology of Mount Ararat/Ağri Daği (Ağri Daği Milli Parki, Eastern Anatolia, Turkey)

ABSTRACT This paper presents a geomorphological map of Mount Ararat/Ağri Daği in Eastern Anatolia (Turkey). Mount Ararat/Ağri Daği is a volcanic complex covered by a unique ice cap in the Near East. The massif is the result of multiple volcanic phases, and present day landforms are the result of subsequent and overlapping glacial, periglacial, and slope processes. The geomorphological mapping of Mount Ararat/Ağri Daği was firstly performed on the basis of desktop studies, by applying remote-sensing investigations using high-resolution satellite imagery (PLEIADES and SPOT images). A preliminary draft of the map was crosschecked and validated in the field as part of an interdisciplinary campaign carried out in the 2014 summer season. All the collected data suggest that the Mount Ararat/Ağri Daği glaciation played a crucial role in the evolution of the landscape and that even today glaciers are significant features in this area. Currently, ice bodies cover 7.28 km2 and include peculiar glacier types. Among these are three well-developed debris-covered glaciers, flowing down along the flanks of the volcano.

Evaluating high-resolution remote sensing data for reconstructing the recent evolution of supra glacial debris: A study in the Central Alps (Stelvio Park, Italy)

Over the last decades, the expansion of supraglacial debris on worldwide mountain glaciers has been reported. Nevertheless, works dealing with the detection and mapping of supraglacial debris and detailed analyses aimed at identifying the temporal and spatial trends affecting glacier debris cover are still limited. In this study, we used different remote sensing sources to detect and map the supraglacial debris cover, to analyze its evolution, and to assess the potential of different remote-sensed image data. We performed our analyses on the glaciers of Ortles-Cevedale Group (Stelvio Park, Italy), one of the most representative glacierized sectors of the European Alps. High-resolution airborne orthophotos (pixel size 0.5 m × 0.5 m) acquired during the summer season in the years 2003, 2007, and 2012 permitted to map in detail, with an error lower than ±5%, the supraglacial debris cover through a maximum likelihood classification. Our findings suggest that over the period 2003–2012, supraglacial debris cover increased from 16.7% to 30.1% of the total glacier area. On Forni Glacier we extended these quantification thanks to the availability of UAV (Unmanned Aerial Vehicle) orthophotos from 2014 and 2015 (pixel size 0.15 m × 0.15 m): this detailed analysis permitted to confirm debris is increasing on the glacier melting surface (+20.4%) and confirms the requirement of high-resolution data in debris mapping on Alpine glaciers. Finally, we also checked the suitability of medium-resolution Landsat ETM+ data and Sentinel 2 data to map debris in a typical Alpine glaciation scenario where small ice bodies (<0.5 km2) are the majority. The results we obtained suggest that medium-resolution data are not suitable for a detailed description and evaluation of supraglacial debris cover in the Alpine scenario, nevertheless Sentinel 2 proved to be appropriate for a preliminary mapping of the main debris features.

Inventory of glaciers and glacial lakes of the Central Karakoram National Park (CKNP – Pakistan)

ABSTRACT This study presents a map reporting valuable information on the cryosphere of the Central Karakoram National Park (CKNP, the largest protected area of Pakistan and the highest park in the world). All the information is provided considering the CKNP as a whole, and in detail by dividing it into five basins (i.e. Shigar, Hunza, Shyok, Upper Indus, and Gilgit). The glacier inventory reports 608 ice bodies covering 3680 km2 (∼35% of the CKNP area), with a total glacier volume of ca. 532 km3. In addition, we modeled the meltwater from glacier ice ablation over the period 23 July to 9 August 2011. The total melt amount is ca. 1.5 km3. Finally, we considered glacial lakes (202 water-bodies, covering 4 km2). For these latter glacier features, we also analyzed their potentially dangerous conditions and two lakes were found having such conditions.