Rodrigo da Silveira Nicoloso

Correlating denitrifying catabolic genes with N2O and N2 emissions from swine slurry composting.

This work evaluated N dynamics that occurs over time within swine slurry composting piles. Real-time quantitative PCR (qPCR) analyzes were conducted to estimate concentrations of bacteria community harboring specific catabolic nitrifying-ammonium monooxygenase (amoA), and denitrifying nitrate- (narG), nitrite- (nirS and nirG), nitric oxide- (norB) and nitrous oxide reductases (nosZ) genes. NH3-N, N2O-N, N2-N emissions represented 15.4 ± 1.9%, 5.4 ± 0.9%, and 79.1 ± 2.0% of the total nitrogen losses, respectively. Among the genes tested, temporal distribution of narG, nirS, and nosZ concentration correlated significantly (p<0.05) with the estimated N2 emissions. Denitrifying catabolic gene ratio (cnorB+qnorB)/nosZ ≥ 100 was indicative of N2O emission potential from the compost pile. Considering our current empirical limitations to accurately measure N2 emissions from swine slurry composting at field scale the use of these catabolic genes could represent a promising monitoring tool to aid minimize our uncertainties on biological N mass balances in these systems.

Carbon stock and its compartments in a subtropical oxisol under long-term tillage and crop rotation systems

Soil organic matter (SOM) plays a crucial role in soil quality and can act as an atmospheric C-CO2 sink under conservationist management systems. This study aimed to evaluate the long-term effects (19 years) of tillage (CT-conventional tillage and NT-no tillage) and crop rotations (R0-monoculture system, R1-winter crop rotation, and R2- intensive crop rotation) on total, particulate and mineral-associated organic carbon (C) stocks of an originally degraded Red Oxisol in Cruz Alta, RS, Southern Brazil. The climate is humid subtropical Cfa 2a (Koppen classification), the mean annual precipitation 1,774 mm and mean annual temperature 19.2 oC. The plots were divided into four segments, of which each was sampled in the layers 0-0.05, 0.05-0.10, 0.10-0.20, and 0.20-0.30 m. Sampling was performed manually by opening small trenches. The SOM pools were determined by physical fractionation. Soil C stocks had a linear relationship with annual crop C inputs, regardless of the tillage systems. Thus, soil disturbance had a minor effect on SOM turnover. In the 0-0.30 m layer, soil C sequestration ranged from 0 to 0.51 Mg ha-1 yr-1, using the CT R0 treatment as base-line; crop rotation systems had more influence on soil stock C than tillage systems. The mean C sequestration rate of the cropping systems was 0.13 Mg ha-1 yr-1 higher in NT than CT. This result was associated to the higher C input by crops due to the improvement in soil quality under long-term no-tillage. The particulate C fraction was a sensitive indicator of soil management quality, while mineral-associated organic C was the main pool of atmospheric C fixed in this clayey Oxisol. The C retention in this stable SOM fraction accounts for 81 and 89 % of total C sequestration in the treatments NT R1 and NT R2, respectively, in relation to the same cropping systems under CT. The highest C management index was observed in NT R2, confirming the capacity of this soil management practice to improve the soil C stock qualitatively in relation to CT R0. The results highlighted the diversification of crop rotation with cover crops as a crucial strategy for atmospheric C-CO2 sequestration and SOM quality improvement in highly weathered subtropical Oxisols.

Assessment of N2O emission from a photobioreactor treating ammonia-rich swine wastewater digestate

This study investigated the interactions between naturally occurring bacteria and the microalgae Chlorella vulgaris within a lab scale photobioreactor treating ammonia-rich swine wastewater digestate effluent. Nitrification and denitrification were assessed by targeting ammonia monoxygenases (amoA), nitrate (narG), nitrite (nirS), nitric oxide (norB) and nitrous oxide (nosZ) reductases genes. Oxygen produced from microalgae photosynthesis stimulated nitrification. Under limiting carbon availability (i.e., <1.44 for mg TOC/mg NO2-N and 1.72 for mg TOC/mg NO3-N), incomplete denitrification led to accumulation of NO2 and NO3. Significant N2O emission (up to 118 μg N2O-N) was linked to NO2 metabolism in Chlorella. The addition of acetate as external carbon source recovered heterotrophic denitrification activity suppressing N2O emission. Effluent methane concentrations trapped within photobioreactor was removed concomitantly with ammonia. Overall, closed photobioreactors can be built to effectively remove nitrogen and mitigate simultaneously greenhouse gases emissions that would occur otherwise in open microalgae-based wastewater treatment systems.

Alterações nos atributos físicos de um latossolo vermelho sob plantio direto induzidas por diferentes tipos de escarificadores e o rendimento da soja

Soil compaction at high intensity degrees is a hardly reversible process in the no-till system (NT), mainly in clayey soils. Generally, its occurrence in croplands is often irregular and restricted to areas with a previous history of pressures associated to intensive traffic of agricultural machineries. The purpose of this study was to investigate changes in soil physical properties of a red Oxisol with clayey texture (450 g kg-1) and soybean yield, caused by two site-specific chisels and conventional (random) chisel tillage. For this work, a 50.6 ha cropland area under long-term no-till and site-specific farming was selected in the Municipality of Victor Graeff, located in the plateau region of Rio Grande do Sul. The climate is a subtropical Cfa with annual rainfall ranging from 1500 to 1750 mm, average temperature of 20 °C, at 490 m asl. Based on three crop yield maps, the field was subdivided in three management zones with distinct yield potential where the soil physical properties were investigated. The following treatments were established in the low yield zone (LZ): (a) conventional chiseling at fixed soil depth (0.30 m) (CC); (b) site-specific chiseling with Fox® equipment at fixed soil depth (0.30 m) (SSCF); (c) site-specific chiseling with Fox® equipment at variable soil depth (0.10 to 0.30 m) based on maximum soil penetration resistance maps (SSCV); and (d) control without chiseling (CWC). The high yield zone (HZ) was used as a reference to evaluate treatment effects on soybean grain yield. Treatments were arranged in parallel strips of 100 x 20 m, in a randomized block design. In three evaluations, the soil water infiltration and bulk density, macro and microporosity and total porosity were evaluated in the layers 0.0-0.05, 0.05-0.10, 0.10-0.15 and 0.15-0.20 m. The results of the initial soil characterization showed higher bulk density and lower soil porosity in the 0.15-0.20 m layer, in the LZ in relation to HZ. Treatments showed an immediate increase of water infiltration after chiseling. The increase in water infiltration in treatments CC and SSCV was four times the water infiltration observed in the CWC. However, the chiseling effect, regardless of the chisel type, was ephemeral and after seven months there was a decrease of water infiltration in chiseled soil, similar to CWC. Of the chisel types, the SSCV was superior to CC by maintaining higher soil coverage and by increasing soybean yield. Although, in a crop season with high rainfall, no positive effects of chiseling on soybean yield were noticed, in relation to CWC.

Effect of 15n-labeled hairy vetch and nitrogen fertilization on maize nutrition and yield under no-tillage¹

This study evaluated the effect of hairy vetch (Vicia villosa Roth) as cover crop on maize nutrition and yield under no tillage using isotope techniques. For this purpose, three experiments were carried out: 1) quantification of biological nitrogen fixation (BNF) in hairy vetch; 2) estimation of the N release rate from hairy vetch residues on the soil surface; 3) quantification of 15N recovery by maize from labeled hairy vetch under three rates of mineral N fertilization. This two-year field experiment was conducted on a sandy Acrisol (FAO soil classification) or Argissolo Vermelho distrofico arenico (Brazilian Soil Classification), at a mean annual temperature of 18 oC and mean annual rainfall of 1686 mm. The experiment was arranged in a double split-plot factorial design with three replications. Two levels of hairy vetch residue (50 and 100 % of the aboveground biomass production) were distributed on the surface of the main plots (5 x 12 m). Maize in the sub-plots (5 x 4 m) was fertilized with three N rates (0, 60, and 120 kg ha-1 N), with urea as N source. The hairy vetch-derived N recovered by maize was evaluated in microplots (1.8 x 2.2 m). The BFN of hairy vetch was on average 72.4 %, which represents an annual input of 130 kg ha-1 of atmospheric N. The N release from hairy vetch residues was fast, with a release of about 90 % of total N within the first four weeks after cover crop management and soil residue application. The recovery of hairy vetch 15N by maize was low, with an average of 12.3 % at harvest. Although hairy vetch was not directly the main source of maize N nutrition, the crop yield reached 8.2 Mg ha-1, without mineral fertilization. There was an apparent synergism between hairy vetch residue application and the mineral N fertilization rate of 60 kg ha-1, confirming the benefits of the combination of organic and inorganic N sources for maize under no tillage.

Long-term C-CO2 emissions and carbon crop residue mineralization in an oxisol under different tillage and crop rotation systems

Soil C-CO2 emissions are sensitive indicators of management system impacts on soil organic matter (SOM). The main soil C-CO2 sources at the soil-plant interface are the decomposition of crop residues, SOM turnover, and respiration of roots and soil biota. The objectives of this study were to evaluate the impacts of tillage and cropping systems on long-term soil C-CO2 emissions and their relationship with carbon (C) mineralization of crop residues. A long-term experiment was conducted in a Red Oxisol in Cruz Alta, RS, Brazil, with subtropical climate Cfa (Koppen classification), mean annual precipitation of 1,774 mm and mean annual temperature of 19.2 o C. Treatments consisted of two tillage systems: (a) conventional tillage (CT) and (b) no tillage (NT) in combination with three cropping systems: (a) R0- monoculture system (soybean/wheat), (b) R1- winter crop rotation (soybean/wheat/soybean/black oat), and (c) R2- intensive crop rotation (soybean/ black oat/soybean/black oat + common vetch/maize/oilseed radish/wheat). The soil C-CO2 efflux was measured every 14 days for two years (48 measurements), by trapping the CO2 in an alkaline solution. The soil gravimetric moisture in the 0–0.05 m layer was determined concomitantly with the C-CO2 efflux measurements. The crop residue C mineralization was evaluated with the mesh-bag method, with sampling 14, 28, 56, 84, 112, and 140 days after the beginning of the evaluation period for C measurements. Four C conservation indexes were used to assess the relation between C-CO2 efflux and soil C stock and its compartments. The crop

Optical crop sensor for variable-rate nitrogen fertilization in corn: i - plant nutrition and dry matter production

Variable-rate nitrogen fertilization (VRF) based on optical spectrometry sensors of crops is a technological innovation capable of improving the nutrient use efficiency (NUE) and mitigate environmental impacts. However, studies addressing fertilization based on crop sensors are still scarce in Brazilian agriculture. This study aims to evaluate the efficiency of an optical crop sensor to assess the nutritional status of corn and compare VRF with the standard strategy of traditional single-rate N fertilization (TSF) used by farmers. With this purpose, three experiments were conducted at different locations in Southern Brazil, in the growing seasons 2008/09 and 2010/11. The following crop properties were evaluated: above-ground dry matter production, nitrogen (N) content, N uptake, relative chlorophyll content (SPAD) reading, and a vegetation index measured by the optical sensor N-Sensor® ALS. The plants were evaluated in the stages V4, V6, V8, V10, V12 and at corn flowering. The experiments had a completely randomized design at three different sites that were analyzed separately. The vegetation index was directly related to above-ground dry matter production (R2 = 0.91; p<0.0001), total N uptake (R2 = 0.87; p<0.0001) and SPAD reading (R2 = 0.63; p<0.0001) and inversely related to plant N content (R2 = 0.53; p<0.0001). The efficiency of VRF for plant nutrition was influenced by the specific climatic conditions of each site. Therefore, the efficiency of the VRF strategy was similar to that of the standard farmer fertilizer strategy at sites 1 and 2. However, at site 3 where the climatic conditions were favorable for corn growth, the use of optical sensors to determine VRF resulted in a 12 % increase in N plant uptake in relation to the standard fertilization, indicating the potential of this technology to improve NUE.

RESIDUAL EFFECT OF SOIL TILLAGE ON WATER EROSION FROM A TYPIC PALEUDALF UNDER LONG-TERM NO- TILLAGE AND CROPPING SYSTEMS (1)

Soil erosion is one of the chief causes of agricultural land degradation. Practices of conservation agriculture, such as no-tillage and cover crops, are the key strategies of soil erosion control. In a long-term experiment on a Typic Paleudalf, we evaluated the temporal changes of soil loss and water runoff rates promoted by the transition from conventional to no-tillage systems in the treatments: bare soil (BS); grassland (GL); winter fallow (WF); intercrop maize and velvet bean (M+VB); intercrop maize and jack bean (M+JB); forage radish as winter cover crop (FR); and winter cover crop consortium ryegrass - common vetch (RG+CV). Intensive soil tillage induced higher soil losses and water runoff rates; these effects persisted for up to three years after the adoption of no-tillage. The planting of cover crops resulted in a faster decrease of soil and water loss rates in the first years after conversion from conventional to no-tillage than to winter fallow. The association of no-tillage with cover crops promoted progressive soil stabilization; after three years, soil losses were similar and water runoff was lower than from grassland soil. In the treatments of cropping systems with cover crops, soil losses were reduced by 99.7 and 66.7 %, compared to bare soil and winter fallow, while the water losses were reduced by 96.8 and 71.8 % in relation to the same treatments, respectively.

CRESCIMENTO E ABSORÇÃO DE NUTRIENTES PELO FEIJOEIRO ADUBADO COM CAMA DE AVES E FERTILIZANTES MINERAIS

Common bean is traditionally grown on small farms in Brazil, where animal wastes are often used as soil fertilizer. Since this is a short-cycle species, nutrients must be available soon after seed germination, which is not always the case when supplied in the form of organic fertilizers. This study was carried out to evaluate the efficiency of different poultry litters in comparison with mineral fertilizers to increase dry matter yield and nutrient release to beans. The experiment was conducted in 2010, in a greenhouse, with Oxisol samples containing16 g kg-1 of organic matter, 1.9 and 84 mg dm-3 of P and K, respectively, and with a pH of 6.0. A randomized complete block design was used with 10 treatments and five replications. The experimental units consisted of 14 dm3 of soil (dry base) in plastic pots, where five seedlings (cultivar BRS Requinte) were grown for 60 days. Treatments consisted of five poultry litters on the materials maize straw, sugar cane bagasse, native grass straw, sand and pine needles, respectively, and four mineral nutrient combinations (NPK, NP, PK, and NK), plus one control without any fertilizer. Mineral fertilizers containing P induced higher dry matter yield of shoot and roots of bean than the poultry litters, due to the release of higher amounts of available N and P. Of the animal wastes, the poultry litter with sand substrate was the most efficient to promote bean yield. Plants fertilized with poultry litters accumulated, on average 58.6 and 59.0 %, respectively, of the N and P accumulated in plants treated with mineral fertilizers containing N and P. The N and K recovery rate by plants was always higher for nutrients applied in mineral than organic forms. Poultry litters may be used as soil fertilizer of common beans provided they are supplemented with mineral fertilizers containing N and P.

Optical crop sensor for variable-rate nitrogen fertilization in corn: II - indices of fertilizer efficiency and corn yield

Generally, in tropical and subtropical agroecosystems, the efficiency of nitrogen (N) fertilization is low, inducing a temporal variability of crop yield, economic losses, and environmental impacts. Variable-rate N fertilization (VRF), based on optical spectrometry crop sensors, could increase the N use efficiency (NUE). The objective of this study was to evaluate the corn grain yield and N fertilization efficiency under VRF determined by an optical sensor in comparison to the traditional single-application N fertilization (TSF). With this purpose, three experiments with no-tillage corn were carried out in the 2008/09 and 2010/11 growing seasons on a Hapludox in South Brazil, in a completely randomized design, at three different sites that were analyzed separately. The following crop properties were evaluated: aboveground dry matter production and quantity of N uptake at corn flowering, grain yield, and vegetation index determined by an N-Sensor® ALS optical sensor. Across the sites, the corn N fertilizer had a positive effect on corn N uptake, resulting in increased corn dry matter and grain yield. However, N fertilization induced lower increases of corn grain yield at site 2, where there was a severe drought during the growing period. The VRF defined by the optical crop sensor increased the apparent N recovery (NRE) and agronomic efficiency of N (NAE) compared to the traditional fertilizer strategy. In the average of sites 1 and 3, which were not affected by drought, VRF promoted an increase of 28.0 and 41.3 % in NAE and NRE, respectively. Despite these results, no increases in corn grain yield were observed by the use of VRF compared to TSF.