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Dive into the research topics where Roland Marquet is active.

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Featured researches published by Roland Marquet.


Journal of Biological Chemistry | 1998

Contacts between Reverse Transcriptase and the Primer Strand Govern the Transition from Initiation to Elongation of HIV-1 Reverse Transcription

Jean Marc Lanchy; Gérard Keith; Stuart F. J. Le Grice; Bernard Ehresmann; Chantal Ehresmann; Roland Marquet

HIV-1 reverse transcriptase (RT) utilizes RNA oligomers to prime DNA synthesis. The initiation of reverse transcription requires specific interactions between HIV-1 RNA, primer tRNA3 Lys , and RT. We have previously shown that extension of an oligodeoxyribonucleotide, a situation that mimicks elongation, is unspecific and differs from initiation by the polymerization rate and dissociation rate of RT from the primer-template complex. Here, we used replication intermediates to analyze the transition from the initiation to the elongation phases. We found that the 2′-hydroxyl group at the 3′ end of tRNA had limited effects on the polymerization and dissociation rate constants. Instead, the polymerization rate increased 3400-fold between addition of the sixth and seventh nucleotide to tRNA3 Lys. The same increase in the polymerization rate was observed when an oligoribonucleotide, but not an oligodeoxyribonucleotide, was used as a primer. In parallel, the dissociation rate of RT from the primer-template complex decreased 30-fold between addition of the 17th and 19th nucleotide to tRNA3 Lys. The polymerization and dissociation rates are most likely governed by interactions of the primer strand with helix αH in the p66 thumb subdomain and the RNase H domain of RT, respectively.


Journal of Biological Chemistry | 2000

Dynamics of the HIV-1 Reverse Transcription Complex during Initiation of DNA Synthesis

Jean Marc Lanchy; Catherine Isel; Gérard Keith; Stuart F. J. Le Grice; Chantal Ehresmann; Bernard Ehresmann; Roland Marquet


Archive | 2007

RNA and DNA Binding Properties of HIV-1 Vif Protein

Serena Bernacchi; Simon Henriet; Philippe Dumas; Jean-Christophe Paillart; Roland Marquet


Archive | 2013

the HIV-1 Vif Protein in Living Cells APOBEC3G Impairs the Multimerization of

Jean-Christophe Paillart; Yves Mély; Roland Marquet; Ludovic Richert; Santiago Guerrero


Archive | 2007

Molecules which Bind to the Dimerization Initiation Site (DIS) of HIV RNA, Their Synthesis and Their Applications as Drugs

Patrick Pale; Jean-Marc Weibel; Anne Bodlenner; Roland Marquet; Jean-Christophe Paillart; Philippe Dumas; Eric Ennifar; Philippe Walter


Archive | 2005

Transfer RNA modifications and DNA editing in HIV-1 reverse transcription

Roland Marquet; Frédéric Dardel


Archive | 2003

Does the HIV-1 primer activation signal interact with tRNA 3 Lys during the initiation of reverse

Chantal Ehresmann; Bernard Ehresmann; Roland Marquet


Archive | 2002

annealed by the nucleocapsid protein: Comparison with the heat-annealed complex

Fabienne Brulé; Roland Marquet; Liwei Rong; Mark A. Wainberg; Bernard P. Roques; Bernard Ehresmann; Chantal Ehresmann


Archive | 2000

during the first strand transfer of HIV-1 reverse transcription

Fabienne Brulé; Guillaume Bec; Gérard Keith; Stuart F. J. Le Grice; Bernard P. Roques; Bernard Ehresmann; Chantal Ehresmann; Roland Marquet


Archive | 2000

Identification of posttranscriptional nucleotide modifications required for efficient initiation of HIV-1 reverse transcription

Carine Tisné; Mickaël Rigourd; Roland Marquet; Chantal Ehresmann; Frédéric Dardel

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Chantal Ehresmann

Centre national de la recherche scientifique

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Bernard Ehresmann

Centre national de la recherche scientifique

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Stuart F. J. Le Grice

National Institutes of Health

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Bernard P. Roques

Paris Descartes University

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Fabienne Brulé

Centre national de la recherche scientifique

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Philippe Dumas

Centre national de la recherche scientifique

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Bernard Ehresmann

Centre national de la recherche scientifique

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Chantal Ehresmann

Centre national de la recherche scientifique

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