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Topics in Current Chemistry | 1998

POLYNUCLEOTIDE ARRAYS FOR GENETIC SEQUENCE ANALYSIS

Rolfe C. Anderson; Robert J. Lipshutz

We describe a new paradigm for genetic analysis based upon high density arrays of polynucleotide probes. Methods for light-directed polynucleotide array synthesis, as well as array packaging, sample preparation, array hybridization, epifluorescence confocal scanning, and data analysis are described. Applications to discovery, genotyping, expression, and resquencing are presented.


Archive | 1998

Advances in Integrated Genetic Analysis

Rolfe C. Anderson; Gregory J. Bogdan; Alex Puski; Xing Su

Genetic analysis is generally comprised of multistep processes. System requirements for integrating these tests are described, including reaction containment, thermal isolation, and reliable fluid-movement control. Functional integrated systems with results for p53 and cytochrome P450 mutation assays are reported.


Archive | 1996

Integrated nucleic acid diagnostic device

Robert J. Lipshutz; Richard P. Rava; Rolfe C. Anderson; Stephen P. A. Fodor


Archive | 1998

Miniaturized genetic analysis systems and methods

Rolfe C. Anderson; Robert J. Lipshutz; Richard P. Rava; Stephen P. A. Fodor


Nucleic Acids Research | 2000

A miniature integrated device for automated multistep genetic assays

Rolfe C. Anderson; Xing Su; Gregory J. Bogdan; Jeffery Fenton


Archive | 2000

Method of manipulating a gas bubble in a microfluidic device

Rolfe C. Anderson; Robert J. Lipshutz; Richard P. Rava; Stephen P. A. Fodor


Archive | 2000

Electronic detection of hybridization on nucleic acid arrays

Rolfe C. Anderson


Archive | 1999

Single-phase amplification of nucleic acids

Xing Su; Rolfe C. Anderson


Archive | 2004

Integrated nucleic acid diagnostic device and method for in-situ confocal microscopy

Robert J. Lipshutz; Richard P. Rava; Rolfe C. Anderson


Archive | 1996

Integrierte nukleinsäure-diagnostik vorrichtung

Rolfe C. Anderson; Stephen P. A. Fodor; Robert J. Lipshutz; Richard P. Rava

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Richard P. Rava

Massachusetts Institute of Technology

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