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Featured researches published by Roman Kolar.


Stem Cell Research | 2013

Reconsidering pluripotency tests: Do we still need teratoma assays?

Christiane Buta; Robert David; Ralf Dressel; Mia Emgård; Christiane Fuchs; Ulrike Gross; Lyn Healy; Jürgen Hescheler; Roman Kolar; Ulrich Martin; Harald Mikkers; Franz-Josef Müller; Rebekka K. Schneider; Andrea Seiler; Horst Spielmann; Georg Weitzer

The induction of teratoma in mice by the transplantation of stem cells into extra-uterine sites has been used as a read-out for cellular pluripotency since the initial description of this phenomenon in 1954. Since then, the teratoma assay has remained the assay of choice to demonstrate pluripotency, gaining prominence during the recent hype surrounding human stem cell research. However, the scientific significance of the teratoma assay has been debated due to the fact that transplanted cells are exposed to a non-physiological environment. Since many mice are used for a result that is heavily questioned, it is time to reconsider the teratoma assay from an ethical point of view. Candidate alternatives to the teratoma assay comprise the directed differentiation of pluripotent stem cells into organotypic cells, differentiation of cells in embryoid bodies, the analysis of pluripotency-associated biomarkers with high correlation to the teratoma forming potential of stem cells, predictive epigenetic footprints, or a combination of these technologies. Each of these assays is capable of addressing one or more aspects of pluripotency, however it is essential that these assays are validated to provide an accepted robust, reproducible alternative. In particular, the rapidly expanding number of human induced pluripotent stem cell lines, requires the development of simple, affordable standardized in vitro and in silico assays to reduce the number of animal experiments performed.


ALTEX-Alternatives to Animal Experimentation | 2012

Inconsistencies in data requirements of EU legislation involving tests on animals.

Kristina Wagner; Bettina Fach; Roman Kolar

European Union (EU) legislation on the protection of animals used for scientific purposes requires that alternative methods must be used instead of animal tests wherever they are available. Unfortunately, this provision is not implemented to its full extent when it comes to risk assessment of chemicals and new products prior to their authorization and placing on the market in the EU. In this study, we screened data requirements of relevant EU law regarding chemicals (REACH), biocides, pesticides, and food safety (Novel Food) and found that data requirements as part of the risk assessment do not always reflect state-of-the-art science and technology. Most of the data requirements we investigated still include testing on animals for many toxicological endpoints, even though more than 40 alternative testing methods accepted at the level of the EU or the OECD are available. This may be due to a multitude of reasons, including a shortage of both manpower to implement existing knowledge and expertise in the field of alternative methods, as well as unclear and misleading statements on the applicability and state of validation of alternative methods. In conclusion, we strongly suggest a homogeneous EU-wide approach for all areas involving risk assessment of substances with the goal of better implementing the 3Rs and complying with Directive 2010/63/EU. This also would streamline data requirements, save costs on various levels, and enhance product safety for consumers.


Atla-alternatives To Laboratory Animals | 1997

Current status and future developments of databases on alternative methods

A. Janusch; M. Van Der Kamp; Bottrill K; Barbara Grune; D. C. Anderson; B. Ekwall; M. Howald; Roman Kolar; H. J. D. Kuiper; Jean Larson; G. Loprieno; U. G. Sauer; A. J. Smith; J.B.F. van der Valk


ALTEX-Alternatives to Animal Experimentation | 2017

Fetal Bovine Serum (FBS): Past – Present – Future

Jan van der Valk; Karen Bieback; Christiane Buta; Brett Cochrane; Wilhelm G. Dirks; Jianan Fu; James J. Hickman; Christiane Hohensee; Roman Kolar; Manfred Liebsch; Francesca Pistollato; Markus Schulz; Daniel Thieme; Tilo Weber; Joachim Wiest; Stefan Winkler; Gerhard Gstraunthaler


ALTEX-Alternatives to Animal Experimentation | 2008

An animal welfare perspective on animal testing of GMO crops.

Roman Kolar; Brigitte Rusche


ALTEX-Alternatives to Animal Experimentation | 2005

[The use of transgenic animals in biomedical research in Germany. Part 1: Status Report 2001-2003].

Sauer Ug; Roman Kolar; Rusche B


ALTEX-Alternatives to Animal Experimentation | 1998

Revised Tables for the Collection of Statistical Information on the Number of Animals used in Experiments and other Scientific Purposes in the European Union

Sauer Ug; Roman Kolar; Rusche B


Toxicology Letters | 2017

Foetal Calf Serum-free media and serum alternatives

Jan van der Valk; Roman Kolar; Gerhard Gstraunthaler


Atla-alternatives To Laboratory Animals | 2000

Application of the Three Rs principle in the compilation of technical Guidance document in support of Directive 98/8/EC concerning the placing of Biocidal products on the market, part I, Guidance on data requirements for active substances and Biocidal products

U. G. Sauer; Manfred Liebsch; Roman Kolar


ALTEX-Alternatives to Animal Experimentation | 1997

Problems in setting up a European statistics on animal experimentation

Roman Kolar; Rusche B; Sauer Ug

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Manfred Liebsch

Federal Institute for Risk Assessment

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Andrea Seiler

Federal Institute for Risk Assessment

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Barbara Grune

Federal Institute for Risk Assessment

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Daniel Thieme

University of Erlangen-Nuremberg

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Horst Spielmann

Free University of Berlin

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Ralf Dressel

University of Göttingen

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