Roman Wenne

Genetic differentiation in hatchery and wild sea trout (Salmo trutta) in the Southern Baltic at microsatellite loci

Sea trout (Salmo trutta) is a commercially important anadromous salmonid species in the Baltic Sea. Sea trout return for spawning to natal rivers (homing). The number of wild smolts migrating to the sea from rivers in Poland decreased from about 1.5 million ca. 70 years ago to less than 100,000 in recent years. This decrease has been caused by deterioration of the environment, regulation of rivers and dam constructions. Stocking activities have been undertaken to enhance naturally occurring populations. Over 1.5 million smolts are released to Polish rivers each year. Variation at microsatellite loci was used to study genetic differentiation between hatchery-produced smolts and wild-caught spawners returning to rivers. Polymorphism at five loci, Ssa197, Ssa171, Ssa85, Str15 and Str73, was assessed. The number of alleles in hatchery populations was lower, whereas heterozygosity levels were similar across all populations. The genetic composition of adult fish returning to the Vistula River in summer was similar to that of the Rega River population. Populations of hatchery-produced smolts originally derived from Vistula River sea trout were also similar to the Rega River sea trout population. The original Vistula River population may still be represented by some winter spawners and by populations in Drweca or tributaries above the Wloclawek dam.

Doubly Uniparental Inheritance Is Associated With High Polymorphism for Rearranged and Recombinant Control Region Haplotypes in Baltic Mytilus trossulus

Many bivalve species, including mussels of the genus Mytilus, are unusual in having two mtDNA genomes, one inherited maternally (the F genome) and the other inherited paternally (the M genome). The sequence differences between the genomes are usually great, indicating ancient divergence predating speciation events. However, in Mytilus trossulus from the Baltic, both genomes are similar to the F genome from the closely related M. edulis. This study analyzed the mtDNA control region structure in male and female Baltic M. trossulus mussels. We show that a great diversity of structural rearrangements is present in both sexes. Sperm samples are dominated by recombinant haplotypes with M. edulis M-like control region segments, some having large duplications. By contrast, the rearranged haplotypes that dominate in eggs lack segments from this M genome. The rearrangements can be explained by a combination of tandem duplication, deletion, and intermolecular recombination. An evolutionary pathway leading to the recombinant haplotypes is suggested. The data are also considered in relation to the hypothesis that the M. edulis M-like control region sequence is necessary to confer the paternal role on genomes that are otherwise F-like.

Mitochondrial DNA heteroplasmy in European populations of the mussel Mytilus trossulus

Mitochondrial DNA (mtDNA) of Mytilus trossulus from the Gulf of Gdansk (southern Baltic) and M. edulis from Swansea Bay, South Wales, UK, collected in 1991, was studied by restriction-enzyme analysis. These two species were more similar to each other in haplotype frequencies than either was to M. galloprovincialis from Britain. M. trossulus resembles M. edulis in having a high frequency of heteroplasmy restricted to males. However, in contrast to M. edulis where restriction-site heteroplasmy predominates, in M. trossulus heteroplasmic individuals possess two genomes which differ in length by up to 3 kilobases.

Comparative Genomics of Marine Mussels (Mytilus spp.) Gender Associated mtDNA: Rapidly Evolving atp8

The unusual mode of mitochondrial DNA inheritance, with two separate: maternal (F) and paternal (M) lineages, gives unique opportunities to study the evolution of the mitochondrial genome. This system was first discovered in the marine mussels Mytilus. The three related species: Mytilus edulis, Mytilus galloprovincialis and Mytilus trossulus form a complex in which the divergence of M and F lineages pre-dates the speciation. The complete mitochondrial genomes of both lineages were known for all species except Pacific M. trossulus. Here we report, for the first time, the complete sequences of both mitochondrial genomes of Pacific M. trossulus, filling the gap. While the reported M and F genomes are highly diverged (26%), they have similar organisation. The only difference is the translocation of one tRNA gene into the long, mosaic control region of the F genome. Consistent presence of an ORF which most likely represents the atp8 gene was confirmed in both genomes. The predicted protein has characteristics expected of the functional atp8 even though the M and F versions are markedly different in length. Comparative analysis involving all three species led to the conclusion that the cause of a faster evolution of atp8 and Mytilus mtDNA in general is most likely the Compensation-Draft Feedback process coupled with relatively relaxed selection in the M lineage. Thus, we postulate that the adaptive changes may have played a role in the emergence of highly diverged, barely recognizable atp8 in Mytilus mussels.

Scottish Mytilus trossulus mussels retain ancestral mitochondrial DNA: Complete sequences of male and female mtDNA genomes

Mytilus trossulus mussels occur in North America and in the Baltic Sea. Recently genetic markers for the three Mytilus subspecies M. edulis, M. galloprovincialis, and M. trossulus, have been detected at Loch Etive in Scotland suggesting mixed ancestry for this population. Of particular interest is the evidence that M. trossulus occurs at Loch Etive because it had not previously been reported in the British Isles. In the present study, analysis of subspecies-specific diagnostic nuclear DNA markers confirms the presence of a high frequency of mussels with M. trossulus ancestry at Loch Etive. The genetic structure suggests hybridisation at an intermediate stage compared with North American populations, where there is little hybridisation, and Baltic populations where there is extensive introgression. This points strongly against a Baltic origin for Loch Etive M. trossulus. The F and M mitochondrial DNA (mtDNA) genomes of Baltic M. trossulus are similar in sequence to the corresponding genomes in M. edulis and believed to be derived by introgression from that subspecies. Both F and M mtDNA genomes are observed at Loch Etive consistent with the presence of doubly uniparental inheritance. Here we provide the complete sequences of the three M. trossulus mtDNA genomes (one F and two M) from Loch Etive. These genomes are extremely similar to the corresponding genomes from ancestral M. trossulus in America but divergent from the genomes for Baltic M. trossulus. This is the first report of ancestral M. trossulus mtDNA genomes in Europe. The F and M genomes are diverged by 26% in nucleotide sequence, similar to other Mytilus F and M genomes. The gene arrangement in the sequenced genomes is also similar to that in other sequenced Mytilus mtDNA genomes. However the two sequenced M genomes differ by 960bp which is caused by a duplication in the main noncoding region (CR). This duplication has not so far been observed in North American populations of M. trossulus. The coding regions of the Loch Etive genomes have no features suggesting that they are other than functional genomes and have K(a)/K(s) values in coding regions less than one indicative of purifying selection. Estimates of divergence times were made for both genomes and are consistent with invasion of Loch Etive by M. trossulus towards the end of the last glacial period.

Genetic biodiversity in the Baltic Sea: species-specific patterns challenge management

Information on spatial and temporal patterns of genetic diversity is a prerequisite to understanding the demography of populations, and is fundamental to successful management and conservation of species. In the sea, it has been observed that oceanographic and other physical forces can constitute barriers to gene flow that may result in similar population genetic structures in different species. Such similarities among species would greatly simplify management of genetic biodiversity. Here, we tested for shared genetic patterns in a complex marine area, the Baltic Sea. We assessed spatial patterns of intraspecific genetic diversity and differentiation in seven ecologically important species of the Baltic ecosystem—Atlantic herring (Clupea harengus), northern pike (Esox lucius), European whitefish (Coregonus lavaretus), three-spined stickleback (Gasterosteus aculeatus), nine-spined stickleback (Pungitius pungitius), blue mussel (Mytilus spp.), and bladderwrack (Fucus vesiculosus). We used nuclear genetic data of putatively neutral microsatellite and SNP loci from samples collected from seven regions throughout the Baltic Sea, and reference samples from North Atlantic areas. Overall, patterns of genetic diversity and differentiation among sampling regions were unique for each species, although all six species with Atlantic samples indicated strong resistence to Atlantic-Baltic gene-flow. Major genetic barriers were not shared among species within the Baltic Sea; most species show genetic heterogeneity, but significant isolation by distance was only detected in pike and whitefish. These species-specific patterns of genetic structure preclude generalizations and emphasize the need to undertake genetic surveys for species separately, and to design management plans taking into consideration the specific structures of each species.

Recombination in Mitochondrial DNA of European Mussels Mytilus

Mitochondrial DNA was long believed to be purely clonal and free from recombination. Major phylogenetic studies still depend on such assumptions. The peculiar genetic system of marine mussels Mytilus in which two divergent mitochondrial genomes exist provides a unique opportunity to study mtDNA recombination. Previous reports showed the existence of a few haplotypes having very strong recombination signal in the control region of mtDNA. Those recombinant variants have been found in a Baltic Sea population of Mytilus trossulus as well as in Mytilus galloprovincialis from the Black Sea. In both cases the mosaic genomes switched their transmission route and have been inherited paternally. In the present study rearranged mtDNA genomes found in all three European Mytilus species are described. The structure of their control region is a result of intra- and intermolecular recombination between mitochondrial genomes. Together with the phylogenetic reconstruction and geographic distribution, this suggests that two interlineage recombination events have occurred in the control region of mtDNA of European mussels Mytilus. Contrary to earlier observations, some of the mosaic genomes do not show any gender bias, which has important implications regarding the transmission and evolution of blue mussel mitochondrial genomes.

Genetic affinities of the bivalve Macoma balthica from the Pacific coast of North America: evidence for recent introduction and historical distribution

The tellinid bivalve Macoma balthica (L.) has an extensive geographic range that reaches from temperate to arctic coastal waters in the North Atlantic and North Pacific oceans. Recent studies have indicated that eastern and western North Atlantic populations are morphologically and genetically different from one another, and that they may have diverged as sibling species. To determine the genetic relationship between M. balthica from the Pacific and Atlantic coasts of North America, populations from each coast were examined at 11 enzyme loci using standard starch gel electrophoresis. Allele frequency data indicate that M. balthica populations from San Francisco Bay, California appear more closely related to western North Atlantic populations than to populations from Oregon. We suggest that San Francisco Bay populations were introduced relatively recently from western North Atlantic populations. The Oregon populations are probably a natural extension of northern populations that occur along Northern Asia and in the eastern North Atlantic.

Mitochondrial DNA variation in populations of the mussel Mytilus trossulus from the Southern Baltic

The mussel Mytilus trossulus is an important component of the Baltic brackish water ecosystem. The genetic structure of mussel (M. trossulus) populations was studied in sites along the Polish coast, Southern Baltic for two segments of mitochondrial DNA (mtDNA). The mode of inheritance of Mytilus mtDNA is termed doubly uniparental; two genomes are passed independently down the female (the F genome) and male (the M genome) lines of descent. The M genome has not been detected at high frequency in M. trossulus, thus the present study focuses on the F genome. PCR and RFLP analysis was used to characterise haplotypes in the coding region ND2-COIII; PCR was used to detect length variants in a major noncoding region. Significant differentiation between populations was observed in the frequency of 24 coding region haplotypes and 14 different length variants. For the three most frequent coding region haplotypes, two (I and III) are associated with the length variants, whereas the third (II) is monomorphic for a single variant of short length. It is suggested that variant II is derived by introgression from a related species, M. edulis, and may be resistant to expansion in the noncoding region. In both regions studied, the Ewens–Watterson test reveals significant deviations from neutrality with an excess of rare variants. This might be due to selection against slightly deleterious variants and is consistent with previously published results for Mytilus taxa. The present study also points towards the potential utility of mtDNA length variation in studies of population differentiation of Mytilus.

Concentration of uranium and thorium in molluscs inhabiting Gdańsk Bay, Baltic sea

Abstract The concentrations of U and Th were determined in the soft tissue and shell of Mytilus edulis, Cardium glaucum, Mya arenaria, Macoma balthica and other benthic invertebrates from Gdansk Bay. Results of these investigations show that the concentrations of U and Th in some molluscs are species specific. For Th, unlike U, significant site-dependent variations in concentration were noted. Results show some dependence in concentrations of metals analysed with size and age of molluscs. The regression analysis of the data indicates that discrimination factors for U with respect to Ca significantly decrease with an increase in the concentration of Ca in the soft tissues.