Ronald D. Cave

Age-related cannibalism and horizontal transmission of a nuclear polyhedrosis virus in larval Spodoptera frugiperda

1. Experiments were carried out to investigate the incidence of cannibalism throughout the larval development of the noctuid moth Spodoptera frugiperda, and to examine the risk of infection from consuming conspecifics infected with a nuclear polyhedrosis virus (SfNPV).

Does cannibalism in Spodoptera frugiperda (Lepidoptera: Noctuidae) reduce the risk of predation?

Abstract The incidence of cannibalism of larval Spodoptera frugiperda (Lepidoptera: Noctuidae) on maize under field conditions was investigated using field cages. Cannibalism was found to account for approximately 40% mortality when maize plants were infested with two or four fourth-instar larvae over a 3-day period. Field trials examined the effect of larval density on the prevalence of natural enemies of S. frugiperda. The abundance of predators (earwigs, staphylinids, other predatory beetles, and Chrysoperla spp.) was significantly greater on maize plants with higher levels of larval feeding damage, while the relationship between predator abundance and number of S. frugiperda larvae per plant was less clear. As larval damage is probably a more reliable indicator of previous larval density than numbers collected at an evaluation, this indicates that predation risk will be greater for larvae living in large groups. Parasitism accounted for 7.1% mortality of larvae in sorghum, and involved six species of Hymenoptera and Tachinidae. There was no effect of larval density or within-plant distribution on the probability of larval attack by parasitoids. The selective benefits of cannibalism, in relation to the risk of predation and parasitism, are discussed.

Effect of weeds on insect pests of maize and their natural enemies in Southern Mexico

A pilot study performed on the Pacific coastal plain of Chiapas, Mexico, focused on the prevalence of maize crop infestation by insect pests, parasitism of pests and the abundance of insect predators in maize plots with weeds compared with plots under a regime of rigorous manual weed control. Sampling was conducted on four occasions at 20, 32, 44 and 56 days post-planting. Infestation of maize by fall armyworm larvae, Spodoptera frugiperda (Lepidoptera: Noctuidae), was more than twice as great in plots with strict weed control compared with weedy plots at 20 days post-planting, but declined thereafter in both treatments. The prevalence of aphid infestation and the abundance of nitidulid beetles were consistently greater in weed-controlled plots. In contrast, the density of beneficial predatory Coleoptera increased significantly in plots with weeds, and it is suggested that this probably explains the lower incidence of pests. S. frugiperda egg masses placed in experimental plots suffered a significantly higher incidence rate of parasitism by Chelonus insularis (Hymenoptera: Braconidae) in clean plots (42.0%) compared with those placed in weedy plots (3.75%); it is suspected that weeds may hinder the location of egg masses by parasitoids. Overall, the presence or absence of weeds had a marked influence on the arthropod community present in maize fields. The weeds did not affect maize plant height, the levels of plant damage or the yield of grain from plants under each type of weed regime, implying that competitive effects of weeds may be offset by greater numbers of beneficial insects in weedy plots. Our pilot study indicates that strict weed control in maize may be unnecessary.

Consequences of interspecific competition on the virulence and genetic composition of a nucleopolyhedrovirus in Spodoptera frugiperda larvae parasitized by Chelonus insularis

Nucleopolyhedroviruses ( Baculoviridae ) are virulent insect pathogens that generally show a high degree of host specificity and have recognized potential as biological insecticides. Whenever viruses are applied for pest control, a proportion of the infected insects will also be parasitized by hymenopteran or dipteran parasitoids and interspecific competition for host resources will occur; the severity of such competition is likely to be modulated to a large degree by the virulence of each type of parasite. We examined the impact of parasitism by the solitary egg-larval endoparasitoid Chelonus insularis (Hymenoptera: Braconidae) on the speed of kill of nucleopolyhedrovirus-infected Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae and the pattern of host growth and virus production in infected and/or parasitized hosts. We also examined the effect of parasitism on the virulence, infectivity and genetic composition of serially passaged virus. Both parasitism and viral infection resulted in a marked reduction in host growth. When third instar larvae were dually parasitized and virus-infected, the growth rate was even more severely affected compared to parasitized larvae. There was a significant increase in virus production in larvae infected at later instars. Interspecific competition resulted in a substantial decrease in pathogen production in parasitized larvae infected at the fourth instar, but not in parasitized larvae infected at earlier instars. The serial passage experiment resulted in the appearance of four distinct genetic isolates of the virus detected by restriction endonuclease analysis. Of the three isolates that appeared in nonparasitized larvae, two showed increased virulence, expressed by mean time to death, and for one of these the infectivity, expressed as LC 50 , was reduced. One isolate that appeared in parasitized larvae (isolate D) had increased virulence and infectivity. Southern blot analysis indicated that virus isolate D was most likely generated by point mutation of a restriction site or by alterations such as duplications, deletions or by recombination of two or more genotypic variants present in the wild-type nucleopolyhedrovirus isolate. Our study provides clear evidence of interspecific competition within the host, since, depending on the timing of inoculation, adverse effects were observed upon both the parasitoid and the virus.

Field tests of predaceous pentatomid pheromones and semiochemistry of Podisus and Supputius species (Heteroptera: Pentatomidae: Asopinae)

ABSTRACT - Traps baited with synthetic pheromones for either Podisusnigrispinus (Dallas) or Supputius cincticeps (Stal) were field tested at the Uni-versidade Federal de Vicosa, Minas Gerais, Brazil, beginning September 7, 1995.No P. nigrispinus were caught but, in September and October, several adultmales and females of P. distinctus (Stal) were caught along with a few adults of S. cincticeps and two females of a tachinid fly parasitoid, Cylindromyia atra Roeder. Chemical analysis of the pheromone gland extract of P. distinctus malesindicated that this species produces a pheromone containing the major com-pounds of P. nigrispinus and S. cincticeps. Therefore, on November 6 a set oftraps was baited with the pheromones of both P. nigrispinus and S. cincticeps, and monitored in the field until February 6, 1996. During this second phase ofthe study, six times more P. distinctus were caught in the combined pheromonetreatment than in traps baited only with the P. nigrispinus pheromone. The phe-nology of these neotropical asopines is like that for

The potential of Chrysoperla rufilabris and Doru taeniatum as agents for dispersal of Spodoptera frugiperda nucleopolyhedrovirus in maize

The behaviour of two abundant predators in Mesoamerican maize crops, Chrysoperla rufilabris larvae and Doru taeniatum adults, towards healthy and nucleopolyhedrovirus‐infected Spodoptera frugiperda larvae was compared. C. rufilabris did not discriminate between healthy and virus‐infected prey, although the mean search time was approximately two times longer towards virus‐infected larvae. In contrast, D. taeniatum directed a greater proportion of their attacks towards virus‐infected prey but there was no significant difference in the search time. Prey consumption time did not differ significantly for each type of prey by either predator, although prey consumption was much faster in D. taeniatum. Viable virus was detected in D. taeniatum faeces up to 3 d after feeding on infected S. frugiperda larvae, whereas virus was inactivated in the gut of C. rufilabris. Both predators were shown to have acidic guts. A field experiment demonstrated that D. taeniatum that had fed on infected prey could contaminate foliage resulting in the transmission of the disease at a low prevalence (4.7%) to S. frugiperda larvae in a field maize crop.