Ronald G. Schultz

Stabilization of DNA:DNA and DNA:RNA duplexes by substitution of 2′-deoxyadenosine with 2′-deoxy-2-aminoadenosine

Abstract Oligonucleotides containing either 2′-deoxyadenosine d(A) or 2′-deoxy-2-aminoadenosine d(A * ) were synthesized and their relative binding affinities to complementary DNA and RNA targets compared. Substitution of dA with dA * resulted in sequence- and target-dependent changes in duplex stability.

Modified ollgonucleotides. Effect of 4 vs 5-atom chimeric internucleoside linkages on duplex stability

Abstract The question whether a four or a five atom non phosphorus based internucleoside link leads to stronger binding of the resulting chimeric oligonucleotides to complementary sequences has been examined by comparing melting temperatures of relevant duplexes.