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Featured researches published by Ronald Santos Silva.


International Journal of Nanomedicine | 2013

In vivo and in vitro evaluation of octyl methoxycinnamate liposomes.

Aline de Carvalho Varjão Mota; Zaida Maria Faria de Freitas; Eduardo Ricci Júnior; Gisela Maria Dellamora-Ortiz; Ralph Santos-Oliveira; Rafael Antonio Ozzetti; André Luiz Vergnanini; Vanessa Lira Ribeiro; Ronald Santos Silva; Elisabete Pereira dos Santos

Solar radiation causes damage to human skin, and photoprotection is the main way to prevent these harmful effects. The development of sunscreen formulations containing nanosystems is of great interest in the pharmaceutical and cosmetic industries because of the many potential benefits. This study aimed to develop and evaluate an octyl methoxycinnamate (OMC) liposomal nanosystem (liposome/OMC) to obtain a sunscreen formulation with improved safety and efficacy by retaining OMC for longer on the stratum corneum. Methods The liposome/OMC nanostructure obtained was tested for enzymatic hydrolysis with lipase from Rhizomucor miehei and biodistribution with liposomes labeled with technetium-99m. The liposome/OMC formulation was then incorporated in a gel formulation and tested for ocular irritation using the hen’s egg test-chorio-allantoic membrane (HET-CAM) assay, in vitro and in vivo sun protection factor, in vitro release profile, skin biometrics, and in vivo tape stripping. Results The liposome/OMC nanosystem was not hydrolyzed from R. miehei by lipase. In the biodistribution assay, the liposome/OMC formulation labeled with technetium-99m had mainly deposited in the skin, while for OMC the main organ was the liver, showing that the liposome had higher affinity for the skin than OMC. The liposome/OMC formulation was classified as nonirritating in the HET-CAM test, indicating good histocompatibility. The formulation containing liposome/OMC had a higher in vivo solar photoprotection factor, but did not show increased water resistance. Inclusion in liposomes was able to slow down the release of OMC from the formulation, with a lower steady-state flux (3.9 ± 0.33 μg/cm2/hour) compared with the conventional formulation (6.3 ± 1.21 μg/cm2/hour). The stripping method showed increased uptake of OMC in the stratum corneum, giving an amount of 22.64 ± 7.55 μg/cm2 of OMC, which was higher than the amount found for the conventional formulation (14.57 ± 2.30 μg/cm2). Conclusion These results indicate that liposomes are superior carriers for OMC, and confer greater safety and efficacy to sunscreen formulations.


Marine Drugs | 2013

Antinociceptive Activity of Stephanolepis hispidus Skin Aqueous Extract Depends Partly on Opioid System Activation

Vinicius F. Carvalho; Lohengrin Fernandes; Taline Ramos Conde; Helena Zamith; Ronald Santos Silva; Andrea Surrage; Valber da Silva Frutuoso; Hugo C. Castro-Faria-Neto; Fabio C. Amendoeira

Stephanolepis hispidus is one of the most common filefish species in Brazil. Its skin is traditionally used as a complementary treatment for inflammatory disorders. However, there are very few studies on chemical and pharmacological properties using the skin of this fish. This study was undertaken in order to investigate the effect of aqueous crude extract of S. hispidus skin (SAE) in different nociception models. Here, we report that intraperitoneal administration of SAE inhibited the abdominal constrictions induced by acetic acid in mice. In addition to the effect seen in the abdominal constriction model, SAE was also able to inhibit the hyperalgesia induced by carrageenan and prostaglandin E2 (PGE2) in mice. This potent antinociceptive effect was observed in the hot plate model too, but not in tail-flick test. Naloxone, an opioid receptor antagonist, was able to block the antinociceptive effect of SAE in the abdominal constriction and hot plate models. In addition, SAE did not present cytotoxic or genotoxic effect in human peripheral blood cells. Our results suggest that aqueous crude extract from S. hispidus skin has antinociceptive activity in close relationship with the partial activation of opioid receptors in the nervous system. Moreover, aqueous crude extract from S. hispidus skin does not present toxicity and is therefore endowed with the potential for pharmacological control of pain.


Archive | 2011

In vitro anti-Leishmania amazonensis activity of the polymeric procyanidin-rich aqueous extract from Syagrus coronata

Igor A. Rodrigues; Daniela Sales Alviano; Marta T. Gomes; Davi Oliveira e Silva; Rosemar Antoniassi; Antonio Jorge; Ronald Santos Silva; Humberto R. Bizzo; Celuta Sales Alviano; Alane Beatriz Vermelho; Maria do Socorro; S. Rosa


Ciência Veterinária nos Trópicos | 2010

Métodos alternativos ao uso de animais: uma visão atual

Octavio Augusto França Presgrave; Cristiane Caldeira; Isabela Gimenes; João Carlos Borges Rolim de Freitas; Saulo de Tasso Borges Nogueira; Nathalia Duque Estrada de Oliveira; Amanda Gleyce Lima de Oliveira; Ronald Santos Silva; Eloisa Nunes Alves; Rosaura de Farias Presgrave


Revista Brasileira De Farmacognosia-brazilian Journal of Pharmacognosy | 2016

In vitro and in vivo evaluation of efficacy and safety of photoprotective formulations containing antioxidant extracts

Maria Cristina.P.P.R. Mansur; Suzana G. Leitão; Cristal Cerqueira-Coutinho; Alane Beatriz Vermelho; Ronald Santos Silva; Octavio Augusto França Presgrave; Alvaro C. Leitão; Gilda Guimarães Leitão; Eduardo Ricci-Júnior; Elisabete Pereira dos Santos


Revista do Instituto Adolfo Lutz (Impresso) | 2012

Ensaios da membrana cório-alantoide (HET-CAM e CAM-TBS): alternativas para a avaliação toxicológica de produtos com baixo potencial de irritação ocular

Amanda Gleyce Lima de Oliveira; Ronald Santos Silva; Eloisa Nunes Alves; Rosaura de Farias Presgrave; Octavio Augusto França Presgrave; Isabella Fernandes Delgado


Archive | 2014

Performance of the chorion-allantoic membrane stained by trypan blue (CAM-TBS) of different concentrations of surfactant sodium dodecyl sulfate (SDS) for assessing the eye irritation potential

Ronald Santos Silva; Vanessa Lira Ribeiro; Cristiane Caldeira da Silva; Octavio Augusto França Presgrave; Isabella Fernandes Delgado


Archive | 2013

Performance do ensaio da membrana córion-alantóide corada pelo azul tripan (CAM-TBS) frente a diferentes concentrações do tensoativo dodecil sulfato de sódio (SDS) para avaliação do potencial irritante ocular de produtos cosméticos e saneantes

Ronald Santos Silva; Vanessa Lira Ribeiro; Cristiane Caldeira da Silva; Octavio Augusto França Presgrave; Isabella Fernandes Delgado


Revista do Instituto Adolfo Lutz (Impresso) | 2012

Chorioallantoic membrane assays (HET-CAM and CAM-TBS): alternative tests for performing toxicological evaluation of products with low potential for ocular irritation

Amanda Gleyce Lima de Oliveira; Ronald Santos Silva; Eloisa Nunes Alves; Rosaura de Farias Presgrave; Octavio Augusto França Presgrave; Isabella Fernandes Delgado


Archive | 2012

Ensaios da membrana cório-alantoide (HET-CAM e CAM-TBS): alternativas para a avaliação toxicológica de produtos com baixo potencial de irritação ocular Chorioallantoic membrane assays (HET-CAM and CAM-TBS): alternative tests for performing toxicological evaluation of products with low potential for ocular irritation

Amanda Gleyce; Lima de Oliveira; Ronald Santos Silva; Eloisa Nunes; Octavio Augusto; França Presgrave; Isabella Fernandes Delgado

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Alane Beatriz Vermelho

Federal University of Rio de Janeiro

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