Rong Qin

Effects of aluminum on nucleoli in root tip cells and selected physiological and biochemical characters in Allium cepa var. agrogarum L

BackgroundIncreased Al concentration causes reduction of mitotic activity, induction of nucleolar alteration, increase of the production of ROS and alteration of several antioxidant enzyme activities in plant cells. Allium cepa is an excellent plant and a useful biomarker for environmental monitoring. Limited information is available about the effects of Al on nucleoli, antioxidant enzyme system, contents of MDA and soluble protein in A. cepa. Therefore, we carried out the investigation in order to better understand the effects of Al on the growth, nucleoli in root tip cells and selected physiological and biochemical characters.ResultsThe results showed that the root growth exposed to 50 μM Al was inhibited significantly. 50 μM Al could induce some particles of argyrophilic proteins scattered in the nuclei and extruded from the nucleoli into the cytoplasm. The nucleolus did not disaggregate normally and still remained its characteristic structure during metaphase. Nucleolar reconstruction was inhibited. 50 μM Al induced high activities of SOD and POD in leaves and roots significantly (P < 0.05) when compared with control, whereas the level of CAT was low significantly (P < 0.05). At 50 μM Al the content of MDA in leaves was high significantly (P < 0.05) at 9th day and in roots increased (P < 0.05) with prolonging the treatment time during 6-12 days. The soluble protein content in leaves treated with 50 μM Al was high significantly (P < 0.05) at 6th day and increased with prolonging the treatment time.ConclusionsWe suggest that variations in nucleoli and the alterations of antioxidant enzyme activities, MDA and soluble protein contents in Allium cepa can serve as useful biomarkers, which can provide valuable information for monitoring and forecasting effects of exposure to Al in real scenarios conditions. Among the antioxidant enzymes SOD and POD appear to play a key role in the antioxidant defense mechanism under Al toxicity condition. Data from MDA concentration show that Al indirectly produces superoxide radicals, resulting in increased lipid peroxidative products and oxidative stress.

Accumulation and cellular toxicity of aluminum in seedling of Pinus massoniana

BackgroundMasson pine (Pinus massoniana) is one of the most important timber species with adaptable, fast growing, versatile advantages in southern China. Despite considerable research efforts, the cellular and molecular mechanisms of A1 toxicity and resistance in P. massoniana are still poorly understood. The effects of Al on uptake and translocation of Al and other minerals, cell division and nucleolus in P. massoniana were investigated.ResultsThe results indicated that Al accumulated mainly in the roots, and small amounts were transported to aboveground organs. In the presence of Al, the contents of Mg and Fe in stems increased and decreased in roots. Accumulation of Mn in the organs was inhibited significantly. Evidence from cellular experiments showed that Al had an inhibitory effect on the root growth at all concentrations (10-5 - 10-2 M) used. Chromosome fragments, chromosome bridges, C-mitosis and chromosome stickiness were induced during mitosis in the root tip cells. Al induced the formation of abnormal microtubule (MT) arrays, consisting of discontinuous wavy MTs or short MT fragments at the cell periphery. MT organization and function of the mitotic spindle and phragmoplast were severely disturbed. The nucleolus did not disaggregate normally and still remained its characteristic structure during metaphase. Nucleolar particles containing argyrophilic proteins were accumulated and leached out from the nucleus to the cytoplasm. Evidence confirmed that these proteins contained nucleophosmin (B23), nucleolin (C23) and fibrillarin. Western immunoblot analysis revealed that the contents of three nucleolar proteins increased significantly.ConclusionBased on the information provided in this article, it is concluded that root tips of plants are the most sensitive organ to environmental stresses and the accumulation of Al ions primarily is in roots of P. massoniana, and small amounts of Al are transported to aboveground. Root apical meristems play a key role in the immediate reaction to stress factors by activating signal cascades to the other plant organs. Al induces a series of the cellular toxic changes concerning with cell division and nucleolus. The data presented above can be also used as valuable and early markers in cellular changes induced by metals for the evaluation of metal contamination.

Determination of Pb genotoxic effects in Allium cepa root cells by fluorescent probe, microtubular immunofluorescence and comet assay

AimsIn order to further understand the toxic effects of Pb on root tip cells of Allium cepa var. agrogarum, root growth, Pb uptake, cell division, DNA damage and MT cytoskeleton were investigated.MethodsFluorescence labeling; Carbol Fuchsin-staining; indirect immunofluorescent microscopy; comet assay.ResultsPb ions entered A. cepa root meristem cells within hours of exposure. The mitotic index (MI) decreased progressively with increasing of Pb concentration. Chromosomal segregation and motility are inhibited, which is directly correlated with the disorganization of the corresponding MT arrays. Pb may interact with tubulin and hinder the polymerization process, resulting in defective mitotic spindles. Pb induced DNA damage in A. cepa root nuclei.ConclusionRoot tips of plants are the most sensitive organ to environmental stresses. Root apical meristems play a key role in the immediate reaction to stress factors by activating signal cascades to the other plant organs. The data presented above can be used as valuable and early markers in cellular changes induced by metals for the evaluation of metal contamination and can be used as early warning signals for general or particular stress.

Effects of Lead on the Morphology and Structure of the Nucleolus in the Root Tip Meristematic Cells of Allium cepa L.

To study the toxic mechanisms of lead (Pb) in plants, the effects of Pb on the morphology and structure of the nucleolus in root tip meristematic cells of Allium cepa var. agrogarum L. were investigated. Fluorescence labeling, silver-stained indirect immunofluorescent microscopy and western blotting were used. Fluorescence labeling showed that Pb ions were localized in the meristematic cells and the uptake and accumulation of Pb increased with treatment time. At low concentrations of Pb (1–10 μM) there were persistent nucleoli in some cells during mitosis, and at high concentration (100 μM) many of the nucleolar organizing regions were localized on sticky chromosomes in metaphase and anaphase cells. Pb induced the release of particles containing argyrophilic proteins to be released from the nucleus into the cytoplasm. These proteins contained nucleophosmin and nucleolin. Pb also caused the extrusion of fibrillarin from the nucleus into the cytoplasm. Western blotting demonstrated the increased expression of these three major nucleolar proteins under Pb stress.

Aluminum can induce alterations in the cellular localization and expression of three major nucleolar proteins in root tip cells of Allium cepa var. agrogarum L.

A 50 μM aluminum (Al) could induce nucleolar materials containing the argyrophilic proteins scattered in the nuclei and extruded from the nuclei into the cytoplasm in the root tip cells of Allium cepa. Unfortunately, what kinds of nucleolar proteins are affected has not been reported till now. In order to go deeper into the understanding of the cytological effects of Al on nucleolus and nucleolar proteins, alterations in the cellular localization and expression of three major nucleolar proteins: nucleophosmin, nucleolin, and fibrillarin were further examined under the treatment with Al in the root tip cells of A. cepa in the present study. Cytological effects of Al on nucleolus were observed by silver-staining method and three major nucleolar proteins: nucleophosmin, nucleolin, and fibrillarin were examined by western blotting. The results indicated that in the presence of 50 μM Al for 48 h the nucleolar proteins were translocated from nucleolus to nucleoplasm and cytoplasm. Western blotting data demonstrated the relatively higher expression of the three major nucleolar proteins when compared with control. Evidence from the present investigation indicated that Al had toxic effects on Ag-NOR proteins, nucleophosmin and nucleolin, and other kinds of nucleolar proteins, fibrillarin.