Rong Shao

Study on Adsorbing Chromium(VI) Ions in Wastewater by Aureobacidium pullulans Secretion of Melanin

Heavy water is a class of highly contaminated waste water, even if the concentration is small, it can cause harm, and the toxicity is long-term sustainability.In this paper, Aureobasidium pullulans secretion of melanin were used for adsorption of Cr6+ ions in waste water.Aureobasidium pullulans strain were activated, cultured for seed and fermented, so that secreted melanin. The Aureobasidium pullulans secretion of melanin were made of dry cell, four factors such as pH, time, amount of bacteria,temperature and three level orthogonal experimental made for adsorption of chromium metal.Results showed, adsorption of chromium metal by dry cells for the best conditions: control temperature 30 , pH 3, the adsorption time was 2 hours, the absorption rate was 27%; and reached the four factors on the adsorption of Cr6+ for Aureobasidium pullulans followed the order: pH> bacteria amount> of time> temperature. In the best adsorption conditions were wet cell assay for adsorption of chromium metal. With 4% (V/V) hydrogen peroxide of 0.01ml/mg bleaching for the dry and wet cell, then for its adsorption of chrome metal. The results showed that the order of effect on adsorption of heavey metal ions by Aureobasidium were: the dry cells secretion of melanin> the wet cells secretion of melanin > decolorized dry cells> decolorized wet cells. It showed that adsorption ability of heavy metals by Aureobasidium pullulans was related to its secretion of melanin.

Study on Antibacterial and Antioxidant Activities of Salicornia herbacea Extracts

This paper researched the bacteriostatic of Salicornia herbacea (SH)extracts by method of spectrophotometry and plate culture, which showed Salicornia herbacea extracts have certain inhibition to both bacteria and streptomyces, and the inhibition rate to bacteria higher than which to streptomyces. At same times, this research studied the antioxidant of Salicornia herbacea extracts on peanut oil by iodometry. And the results showed that the POV (peroxide value) which added a certain amount of Salicornia herbacea extracts to peanut oil were smaller than the reference.

Optimization of solid state fermentation media of Hericium erinaceus for soluble dietary fiber using artificial neural networks and genetic algorithms

For obtaining maximum soluble dietary fiber (SDF)xa0productionxa0under solid state fermentation (SSF)xa0byxa0Hericium erinaceus,xa0the optimum media supplemented to the basic substrate wheat bran (WB) was investigated. Soybean meal suitable for mycelial growth was used as nitrogen source, along with four inorganic salts (KH2PO4,xa0ZnSO4,xa0FeSO4xa0and MgCl2) that could increase SDF production significantly.xa0Artificial neural networks (ANNs) and genetic algorithms (GA)xa0were used for modeling the non-linear relationship between the fermentation media and SDF production and optimizing the model, respectively. After optimization, parameters of the five media supplemented to WB were (mg g-1xa0WB):xa0soybean mealxa0124.3, KH2PO4xa00.18, ZnSO4xa00.6, FeSO4xa00.2 and MgCl2, 1.2.xa0The SDF production increased to 13.06xa0± 0.51xa0g 100 g-1xa0in the validation experiment, which wasxa04.68 foldxa0as compared withxa0thexa0control. n n xa0 n n Key words:xa0Soluble dietary fiber, solid state fermentation, artificial neural networks, genetic algorithms, optimization.

Optimization of Solid State Fermentation Media for Soluble Dietary Fiber Production by Hericium erinaceus

In this paper, the optimum media supplemented to the basic substrate wheat bran (WB) for getting maximum soluble dietary fiber (SDF) production under solid state fermentation (SSF) by Hericium erinaceus was investigated. Soybean meal was suitable for mycelial growth as nitrogen source, four inorganic salts (KH2PO4、ZnSO4、FeSO4 and MgCl2) could increase SDF production significantly. Artificial neural networks (ANNs) and genetic algorithms (GA) formalisms were used for modeling the non-linear relationship between the fermentation media and SDF production and optimizing the model, respectively. After optimization, parameters of the five media supplemented to WB were (mg g-1 WB): soybean meal 124.3, KH2PO4 0.18, ZnSO4 0.6, FeSO4 0.2, MgCl2, 1.2. The SDF production increased to 13.06±0.51 g 100g-1 in the validation experiment, which was 4.68 fold as compared with control.