Rosa Daza

The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: an NTM-NET collaborative study

A significant knowledge gap exists concerning the geographical distribution of nontuberculous mycobacteria (NTM) isolation worldwide. To provide a snapshot of NTM species distribution, global partners in the NTM-Network European Trials Group (NET) framework (www.ntm-net.org), a branch of the Tuberculosis Network European Trials Group (TB-NET), provided identification results of the total number of patients in 2008 in whom NTM were isolated from pulmonary samples. From these data, we visualised the relative distribution of the different NTM found per continent and per country. We received species identification data for 20 182 patients, from 62 laboratories in 30 countries across six continents. 91 different NTM species were isolated. Mycobacterium avium complex (MAC) bacteria predominated in most countries, followed by M. gordonae and M. xenopi. Important differences in geographical distribution of MAC species as well as M. xenopi, M. kansasii and rapid-growing mycobacteria were observed. This snapshot demonstrates that the species distribution among NTM isolates from pulmonary specimens in the year 2008 differed by continent and differed by country within these continents. These differences in species distribution may partly determine the frequency and manifestations of pulmonary NTM disease in each geographical location. Species distribution among nontuberculous mycobacteria isolates from pulmonary specimens is geographically diverse http://ow.ly/npu6r

Tissular changes induced by Pseudomonas aeruginosa in an otitis media rat model with tubal obstruction

Conclusions. This is a suitable model for the study of different features of middle ear inflammation. This model allows manipulations inside the middle ear while preserving relevant structures such as the tympanic membrane, and provides a useful model for the study of interactions between bacterial infection and eustachian tube dysfunction. Objectives. Analysis of early and late histological features in an experimental model of Pseudomonas aeruginosa middle ear inoculation in the rat designed for the study of middle ear procedures. Materials and methods. Thirty Wistar rats were inoculated with Pseudomonas aeruginosa in the tympanic bulla followed by the cauterization of the eustachian tube. Culturing of middle ear effusion was carried out at 7 days follow-up and at sacrifice. Processing of the temporal bones for light microscopy was performed at 7, 14, 30 and 60 days. Results. Early cultures were positive in most cases, thus proving that middle ear inflammation was due to the presence of inoculated Pseudomona aeruginosa. Mucoperiosteal inflammatory changes similar to those observed in human middle ear infection were seen. Acute inflammatory cell infiltration was seen at 7 and 14 days, gradually decreasing to chronic inflammatory changes with fibroplasia at 60 days. Bone resorption was observed at 7 and 14 days, changing to a bony deposition at 30 and 60 days.

The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples

A significant knowledge gap exists concerning the geographical distribution of nontuberculous mycobacteria (NTM) isolation worldwide. To provide a snapshot of NTM species distribution, global partners in the NTM-Network European Trials Group (NET) framework (www.ntm-net.org), a branch of the Tuberculosis Network European Trials Group (TB-NET), provided identification results of the total number of patients in 2008 in whom NTM were isolated from pulmonary samples. From these data, we visualised the relative distribution of the different NTM found per continent and per country. We received species identification data for 20 182 patients, from 62 laboratories in 30 countries across six continents. 91 different NTM species were isolated. Mycobacterium avium complex (MAC) bacteria predominated in most countries, followed by M. gordonae and M. xenopi. Important differences in geographical distribution of MAC species as well as M. xenopi, M. kansasii and rapid-growing mycobacteria were observed. This snapshot demonstrates that the species distribution among NTM isolates from pulmonary specimens in the year 2008 differed by continent and differed by country within these continents. These differences in species distribution may partly determine the frequency and manifestations of pulmonary NTM disease in each geographical location. Species distribution among nontuberculous mycobacteria isolates from pulmonary specimens is geographically diverse http://ow.ly/npu6r

Prolonged Survival of an HIV-Infected Patient with Multidrug-Resistant Mycobacterium bovis Infection Treated with Surgical Resection

We describe a case of multidrug-resistant tuberculosis due to Mycobacterium bovis in a human immunodeficiency virus-infected woman with good immunologic status. The patient presented with a hard mass measuring 10 cm in diameter on the lower left ribs and a lung nodule measuring 3 cm in diameter in the left superior lobe. No adequate pharmacological treatment was available. Both lesions were surgically resected. The patient has remained asymptomatic (without fever, cough, lymphadenopathy, or cutaneous masses) for 20 months, after discharge from the hospital.

Aparición de nódulos subcutáneos después de aplicación de mesoterapia

Servicio de Medicina Interna, Unidad de Infecciosas, Hospital Universitario Puerta de Hierro, Universidad Autonoma de Madrid, Majadahonda, ed Espanola para la Investigacion de la Patologia Infecciosa, Madrid, Espana Servicio de Dermatologia, Hospital Universitario Puerta de Hierro, Universidad Autonoma de Madrid, Majadahonda, Madrid, Espana Servicio de Cirugia General y Aparato Digestivo, Hospital Universitario Puerta de Hierro, Universidad Autonoma de Madrid, Majadahonda, Madrid, Espana Servicio de Microbiologia, Hospital Universitario Puerta de Hierro, Universidad Autonoma de Madrid, Majadahonda, Madrid, Espana

The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples An NTM-NET collaborative study

A significant knowledge gap exists concerning the geographical distribution of nontuberculous mycobacteria (NTM) isolation worldwide. To provide a snapshot of NTM species distribution, global partners in the NTM-Network European Trials Group (NET) framework (www.ntm-net.org), a branch of the Tuberculosis Network European Trials Group (TB-NET), provided identification results of the total number of patients in 2008 in whom NTM were isolated from pulmonary samples. From these data, we visualised the relative distribution of the different NTM found per continent and per country. We received species identification data for 20 182 patients, from 62 laboratories in 30 countries across six continents. 91 different NTM species were isolated. Mycobacterium avium complex (MAC) bacteria predominated in most countries, followed by M. gordonae and M. xenopi. Important differences in geographical distribution of MAC species as well as M. xenopi, M. kansasii and rapid-growing mycobacteria were observed. This snapshot demonstrates that the species distribution among NTM isolates from pulmonary specimens in the year 2008 differed by continent and differed by country within these continents. These differences in species distribution may partly determine the frequency and manifestations of pulmonary NTM disease in each geographical location. Species distribution among nontuberculous mycobacteria isolates from pulmonary specimens is geographically diverse http://ow.ly/npu6r

Fiebre y lesiones cavitadas pulmonares con pared fina

Varon de 26 anos, estudiante, bebedor moderado de fin de semana (menos de 40g/dia), no fumador, con antecedentes de hepatitis A y asma extrinseco sin tratamiento corticoideo sistemico o inhalado en los ultimos 3 anos, que acudio a la consulta por fiebre de predominio nocturno, sin referir otros sintomas o signos. No se hallaron alteraciones destacables en la exploracion fisica, y tanto el hemograma como la bioquimica sanguinea, el estudio de hormonas tiroideas y las serologias para virus hepatotropos y virus de la inmunodeficiencia humana resultaron normales o negativos. En la radiografia de torax (fig. 1) se observaron una leve perdida de volumen en el lobulo superior izquierdo con distorsion vascular y 2 cavidades de pared fina (fig. 2) junto con un patron reticulonodular en la lingula y en el lobulo inferior izquierdo y un pequeno infiltrado en el campo medio derecho. La tincion de Ziehl-Neelsen en esputo puso de manifiesto la presencia de bacilos resistentes al acido y alcohol. Los esputos se sembraron en medios liquidos (Bactec MGIT 960) y en medio solido (Lowenstein-Jensen). Se inicio tratamiento antituberculoso oral con 300mg/dia de isoniacida, 600mg/dia de rifampicina y 1.500mg/dia de pirazinamida. A los 6 dias se detecto crecimiento en los medios liquidos y se identificaron los microorganismos como Mycobacterium kansasii por medio de sondas comerciales que detectan secuencias especificas de acido ribonucleico ribosomico de M. kansasii (Accuprobe, GenProbe, BioMerieux), asi como por amplificacion y posterior hibridacion reversa basada en tecnologia DNA-STRIP de GenoType Mycobacterium CM. A las 3 semanas se observaron en los tubos de LowensteinJensen colonias lisas, que despues de inducirlas a la luz resultaron ser fotocromogenas.

Morphological sequence of Plastipore extrusion in experimental otitis media.

Objectives: Plastipore prostheses are still used by many surgeons, although the functional results are controversial. The aim of this study was the morphological analysis of Plastipore material performance in the middle ear of rats, with special attention to extrusion. Methods: Twenty-four Wistar rats were given implants made of commercially available Plastipore and assigned to 3 groups: Group A, with implantation in a healthy middle ear; group B, with implantation and cauterization of the nasopharyngeal orifice of the eustachian tube (hypoventilation); and group C, with implantation, cauterization of the eustachian tube, and bacterial inoculation with Pseudomonas aeruginosa. Results: The pathological study showed in nearly all cases the disintegration of the biomaterial. Adhesion between the biomaterial and bone could be seen in 1 rat from group C (hypoventilation and infection). In group C, the Plastipore was in contact with the tympanic membrane in 1 case and was extruding in 2 animals. Different phases of extrusion were defined. No extrusion was observed in the other groups. Conclusions: The sequential stages of Plastipore extrusion are demonstrated. Infection seems to be the most important factor in Plastipore extrusion in our model.