Rosa Negrete

Active constituents isolated from Psoralea glandulosa L. with antiinflammatory and antipyretic activities

The antiinflammatory and antipyretic activities of the petroleum ether extract (PEE), dichloromethane extract (DME) and methanol extract (ME1) of the aerial part of Psoralea glandulosa L. (Papilionaceae) were studied. The bioactivity-guided fractionation of the active extracts yielded the isolation of bakuchiol (Bk) from the petroleum ether as the active compound, cyclobakuchiols A and B (Cbk), and angelicin (Ang) from DME.

Effect of bakuchiol on leukocyte functions and some inflammatory responses in mice

The effects of bakuchiol, a meroterpenoid isolated from the leaves of Psoralea glandulosa L., on phospholipase A2 (PLA2) activity from different sources, human neutrophil responses, zymosan air pouch and topical inflammation in mice, were investigated.

Antinociceptive activity of Buddleja globosa (matico) in several models of pain

ETHNOPHARMACOLOGICAL RELEVANCE Leaf extracts of Buddleja globosa (Buddlejaceae) are used in Chilean folk medicine for wound healing. The anti-inflammatory (topic and per os), analgesic (per os) effects and the antioxidant activity of Buddleja globosa were for the first time reported by us. AIM OF THE STUDY Assess the antinociceptive activity of the methanol sequential and global extracts using complementary chemical and thermal models of pain, characterize pharmacologically the antinociception induced, evaluate seasonal influence to support Buddleja globosa medicinal use. MATERIALS AND METHODS Global methanol, sequential methanol and ethanol (leaves collected in autumn and summer) extracts were evaluated for oral and topic analgesia in tail flick, formalin and writhing models, verbascoside and 7-O-luteolin glucoside were assayed in tail flick and writhing. Ibuprofen was used as reference. For characterization of induced antinociception, naltrexone, naltrindole, tropisetron, nor-binaltorphimine, prazosin, yohimbine, atropine, and N-nitro-l-arginine methyl ester were used as antagonists and inhibitors drugs. RESULTS Seasonal influence was observed since autumn extract resulted less active. Extracts showed a dose-dependent antinociceptive activity in all assays, the highest effects were obtained for the formalin and writhing test. Verbascoside was more active than ibuprofen in the writhing test (67.6% and 50.0% at equimolar doses) and showed similar effects in the tail flick (topic and oral) near 25% at equivalent doses - ED25 or EC25 - to ibuprofen. Luteolin 7-O-glucoside was slightly more active in the tail flick test and nearly half active than verbascoside in the writhing assay. Effectiveness was higher for the sequential than for global alcoholic extracts, and can be increased by selective blocking of opioid receptors. Global methanol extract seems modulated only by naltrexone. CONCLUSIONS Analgesic effect of Buddleja globosa is here demonstrated validating its use in traditional medicine. Season influence is important to be considered.

Antipyretic, Hypothermic and Antiinflammatory Activities and Metabolites from Solanum ligustrinum Lood.

The acute toxicities of the global methanol extract of Solanum ligustrinum (Solanaceae) and the crude steroidal glycoalkaloids mixture were determined. The antipyretic, hypothermic and antiinflammatory activities of aqueous, global methanol, petroleum ether, dichloromethane and methanol extracts and crude steroidal glycoalkaloids mixture of the aerial parts were evaluated.

Insecticide and acaricide activities of polyhalogenated monoterpenes from chilean Plocamium cartilagineum

Abstract Four new alicyclic monoterpenes based on the 1-(2-chlorovinyl)-2,4,5-trichloro-1,5-dimethylcyclohexane skeleton were isolated from Plocamium cartilagineum from the Chilean coast, together with four known cyclic monoterpenes. The structure of the new compounds were determined by comparison of the spectral data with those of known compounds. The insecticide/acaricide and fungicide activities of some of the isolated monoterpenes were determined.

Pharmaco-toxicological study of Kageneckia oblonga, Rosaceae

The probable antipyretic, antiinflammatory, analgesic and antioxidant properties of Kageneckia oblonga, Rosaceae, were investigated and the major compounds of its active extracts were isolated. The study comprised the acute toxicity of the extracts of global methanol, hexane, dichloromethane and methanol. The cytotoxicity of global methanol extract was studied in three tumoral cell lines. All the extracts exhibited the pharmacological activities under study. Methanol and dichloromethane were the most toxic extracts. From the global methanol extract, isolations were performed of prunasin, 23,24- dihydro-cucurbitacin F, and a new cucurbitacin, 3β-(β-d-glucosyloxy)-16α,23α-epoxycucurbita-5,24-diene-11-one. The cytotoxicity of both cucurbitacins on human neutrophils at the assayed concentrations was not statistically significant. In-vitro assays showed that both cucurbitacins can be partly responsible for the analgesic, antipyretic, and anti-inflammatory activities. Evaluation was done of the cytotoxicity of global methanol extract, 23, 24-dihydrocucurbitacin F, aqueous extracts and prunasin against P-388 murine leukaemia, A-549 human lung carcinoma and HT-29 colon carcinoma. Since global methanol extract presented a strong cytotoxicity against P-388 murine leukaemia, A-549 human lung carcinoma, and HT-29 cell lines, it is highly probable that this extract contain one or more cytotoxic compounds that could be investigated for their potential use as an agent against cancer.

Cyclobakuchiols A and B from Psoralea Glandulosa

Bioactivity guided fractionation of the dichloromethane extract from Psoralea glandulosa yielded the isolation of a diasteromeric mixture of cyclobakuchiols A and B as possible anti-inflammatory active compounds.

(+)-9α-hydroxymatrine from Sophora macrocarpa

Abstract A new quinolizidine alkaloid, (+)-9α-hydroxymatrine, was isolated from the leaves of Sophora macrocarpa . Its structure was determined by a combination of spectroscopic methods, among which nuclear Overhauser enhancement experiments played a crucial role. Some generalizations are made regarding the mass spectra of matrinoids.

Biological activities and metabolites from Trevoa trinervis Miers

Chilean folklore medicine uses Trevoa trinervis Miers. (Rhamnaceae), ‘trevo’, for the treatment of inflammation caused by wounds and burns. The antiinflammatory and antipyretic activities of crude methanol, aqueous and successive hexane, dichloromethane, methanol extracts of the aerial part were studied. The fractionation of the most potent extract (dichloromethane), led to the isolation of the pentacyclic triterpenoids betulinic (the major metabolite), oleanolic and ursolic acids, along with β‐sitosterol. Evaluation of the acute oral toxicity of the crude methanol extract showed it to be nontoxic. The crude methanol and the aqueous extracts were assayed for their antineoplastic activity against P‐388 murine leukaemia, A‐549 human lung carcinoma and HT‐29 colon carcinoma showing no activity. Betulinic acid was also assayed showing some activity against the neoplastic cell lines, but only residual activity against Mel‐28 melanona cells.

ANTIMICROBIAL ACTIVITY OF PSORALEA GLANDULOSA L.

AbstractAntimicrobial activity of leaf extracts of Psoralea glandulosa L. (Papilionaceae) is reported. This study was carried out on the extracts and on the plants most abundant metabolite, bakuchiol. Antimicrobial activity against Gram. positive bacteria was observed. Bioautographic assays showed that bakuchiol was the compound responsible for this activity.