Rosemary L. Pendleton

Growth and Nutrient Content of Herbaceous Seedlings Associated with Biological Soil Crusts

Biological soil crusts of arid and semiarid lands contribute significantly to ecosystem stability by means of soil stabilization, nitrogen fixation, and improved growth and establishment of vascular plant species. In this study, we examined growth and nutrient content of Bromus tectorum, Elymus elymoides, Gaillardia pulchella, and Sphaeralcea munroana grown in soil amended with one of three levels of biological soil crust material: (1) a low-fertility sand collected near Moab, Utah; (2) sand amended with a 1-cm top layer of excised soil crust; and (3) crushed crust material. In addition, all plants were inoculated with spores of the arbuscular mycorrhizal fungus, Glomus intraradices. Plants were harvested after 10 weeks growth, dried, weighed, and leaves were ground for nutrient analysis. Three aspects of root architecture were also quantified. Soil crust additions significantly affected nearly all variables examined. Both above- and below-ground vegetative biomass were significantly increased in the presence of crust material. Similarly, reproductive tissue of the three species that flowered was greatest in the crushed-crust medium. The effect of soil crust additions is likely due to the increased nitrogen content of the crusts. Nitrogen tissue content of all four species was greatly enhanced in crusted soils. All species showed a decline in root/shoot ratio and specific root length with crust additions, indicating a shift in plant allocation pattern in response to improved soil fertility. These data support other studies suggesting that soil crusts have a positive effect on the establishment and growth of associated vascular plant species.

Response of Two Semiarid Grasslands to a Second Fire Application

Abstract Prescribed fire was used in two semiarid grasslands to reduce shrub cover, promote grass production, and reduce erosional loss that represents a potential non–point-source of sediment to degrade water quality. This study measured transported soil sediment, dynamics in soil surface microtopography, cover of the woody shrub, grass, and bare ground cover classes, and soil fertility measured by nitrogen-mineralization potentials for the respective cover classes over a 9-year period during which 2 fires occurred. In general, the effects of two drought periods were equal to or greater than the effects of fire on the measured parameters. Following the second fire at the grama grass-dominated site, the number of live junipers was significantly lowered (P < 0.001). Fire consumed grass and shrub cover, which created the potential for greater transport of sediment and loss in soil surface elevation. Soil fertility under shrubs was significantly higher than soil fertility under grasses in both grassland sites before and after the first fire; however, that highly significant pattern was not present after the second fire for about 2 years at both sites. This suggests that the reestablishment of a natural fire frequency would likely dampen the well-documented pattern of greater soil fertility under shrub islands relative to grass islands. Repeat use of prescribed fire may keep shrub cover in check and promote fundamental changes in soil processes within semiarid grasslands, but its value must be weighed against the increased potential for erosion following fire.

Gender specialization in heterodichogamous Grayia brandegei (Chenopodiaceae): evidence for an alternative pathway to dioecy

We examined components of male and female reproductive success in protogynous and protandrous sexual morphs of the heterodichogamous and largely monoecious chenopod shrub Grayia brandegei. Percentage femaleness of flowering stalks ranged from 0 to 37.6% female ( = 15.5%) for protandrous plants and from 14 to 100% female ( = 55.8%) for protogynous plants. Functional gender estimates based on ovule production at two locations ranged from 23.0 to 31.8% female for the protandrous morph, and from 65.3 to 77.0% female for the protogynous morph. Realized gender estimates based on total seed production ranged in value from 3.6 to 16.8% female for the protandrous morph and from 76.5 to 96.4% for the protogynous morph, depending on location and year. Differences in reproductive success of the two morphs were largely due to a reduction in the female function of protandrous plants. Protogynous plants produced more female flowers per stalk and had a higher percentage of seed-filled fruits than did protandrous plants. Differences between sexual morphs were more pronounced in dry areas or years in which overall seed production was minimal. Differential seed production between morphs likely reflects temporal patchiness in environmental conditions, particularly in water availability. The significance of these findings in support of heterodichogamy as an evolutionary pathway to dioecy is discussed.

Adaptive responses reveal contemporary and future ecotypes in a desert shrub

Interacting threats to ecosystem function, including climate change, wildfire, and invasive species necessitate native plant restoration in desert ecosystems. However, native plant restoration efforts often remain unguided by ecological genetic information. Given that many ecosystems are in flux from climate change, restoration plans need to account for both contemporary and future climates when choosing seed sources. In this study we analyze vegetative responses, including mortality, growth, and carbon isotope ratios in two blackbrush (Coleogyne ramosissima) common gardens that included 26 populations from a range-wide collection. This shrub occupies ecotones between the warm and cold deserts of Mojave and Colorado Plateau ecoregions in western North America. The variation observed in the vegetative responses of blackbrush populations was principally explained by grouping populations by ecoregions and by regression with site-specific climate variables. Aridity weighted by winter minimum temperatures best explained vegetative responses; Colorado Plateau sites were usually colder and drier than Mojave sites. The relationship between climate and vegetative response was mapped within the boundaries of the species-climate space projected for the contemporary climate and for the decade surrounding 2060. The mapped ecological genetic pattern showed that genetic variation could be classified into cool-adapted and warm-adapted ecotypes, with populations often separated by steep dines. These transitions are predicted to occur in both the Mojave Desert and Colorado Plateau ecoregions. While under contemporary conditions the warm-adapted ecotype occupies the majority of climate space, climate projections predict that the cool-adapted ecotype could prevail as the dominant ecotype as the climate space of blackbrush expands into higher elevations and latitudes. This study provides the framework for delineating climate change-responsive seed transfer guidelines, which are needed to inform restoration and management planning. We propose four transfer zones in blackbrush that correspond to areas currently dominated by cool-adapted and warm-adapted ecotypes in each of the two ecoregions.

Preparing and staining mycorrhizal structures in dry bean, sweet corn, and wheat using a block digester

Abstract The use of safe staining techniques in the evaluation of mycorrhizal colonization is critical to the continued understanding of this important symbiosis. Several procedures being utilized currently involve regulated and/ or toxic chemicals. The integration of unregulated and nontoxic chemicals into these procedures is important to alleviate potential dangers currently used chemicals pose. We eliminated all regulated reagents by combining portions of several previously published staining and root preservation procedures. A block digester for plant tissue digestion was used as a heating unit and proved to be easier to use, quicker and more reliable than either a water bath or a circulating air oven. Optimum clearing time in KOH varied from 8 to 10 min and 30 to 40 min for wheat, sweet corn, and dry bean roots, respectively. We also successfully used both drying and freezing of roots for storage prior to staining. These modified procedures were quick and easy and provided reliable temperature contro...

Revisiting the Life Cycle of Dung Fungi, Including Sordaria fimicola

Dung fungi, such as Sordaria fimicola, generally reproduce sexually with ascospores discharged from mammalian dung after passage through herbivores. Their life cycle is thought to be obligate to dung, and thus their ascospores in Quaternary sediments have been interpreted as evidence of past mammalian herbivore activity. Reports of dung fungi as endophytes would seem to challenge the view that they are obligate to dung. However, endophyte status is controversial because surface-sterilization protocols could fail to kill dung fungus ascospores stuck to the plant surface. Thus, we first tested the ability of representative isolates of three common genera of dung fungi to affect plant growth and fecundity given that significant effects on plant fitness could not result from ascospores merely stuck to the plant surface. Isolates of S. fimicola, Preussia sp., and Sporormiella sp. reduced growth and fecundity of two of three populations of Bromus tectorum, the host from which they had been isolated. In further work with S. fimicola we showed that inoculations of roots of B. tectorum led to some colonization of aboveground tissues. The same isolate of S. fimicola reproduced sexually on inoculated host plant tissues as well as in dung after passage through sheep, thus demonstrating a facultative rather than an obligate life cycle. Finally, plants inoculated with S. fimicola were not preferred by sheep; preference had been expected if the fungus were obligate to dung. Overall, these findings make us question the assumption that these fungi are obligate to dung.

Origin of the Flax Cultivar ‘Appar’ and Its Position within the Linum perenne Complex

The ‘Appar’ flax germplasm was originally released by the USDA Soil Conservation Service as a cultivar of Linum lewisii. The observation that ‘Appar’ is heterostylic, a key taxonomic character distinguishing Lewis flax from other members of the Linum perenne complex, created a need for further study in order to resolve the taxonomic position of both Lewis flax and the ‘Appar’ cultivar. We grew 10 plants each of nine European, nine North American, and three putative ‘Appar’ seed sources, measured 18 morphologic variables on each plant, and examined genetic variation using randomly amplified polymorphic DNA (RAPD). We also performed self‐ and cross‐pollinations within and among source groups. North American collections differed from European collections for 12 of the 18 morphologic variables. ‘Appar’ collections were morphologically similar to European collections and differed from North American collections for 13 of the 18 variables. RAPD data also showed that ‘Appar’ sources clustered with European, and both were distinct from North American sources. European and ‘Appar’ collections were self‐sterile and interfertile, whereas North American collections were self‐fertile but reproductively isolated from European and ‘Appar’ collections. We conclude that L. lewisii is a distinct species within the L. perenne complex and that ‘Appar’ should be classified as L. perenne. This study provides an example of how questions regarding origin and/or taxonomy of plant materials developed for restoration can be resolved.

Applying the Successional Weed Management Model for Revegetating a Yellow Starthistle-Infested Dryland Pasture in the Chihuahuan Desert

A three-year study was conducted in the Chihuahuan Desert in Southwestern New Mexico to evaluate the effectiveness of revegetating a dryland pasture that was heavily infested with yellow starthistle within the context of the successional weed management model. A prescribed burn treatment of the entire study site (designed disturbance) was followed by single-entry revegetation (controlled colonization) and weed suppression (controlled species performance) treatments. Four native perennial grass species were paired with 4 yellow starthistle suppression treatments. We conclude that an integrated, single-entry approach failed to effectively revegetate yellow starthistle-infested dryland pasture in the Chihuahuan Desert, primarily due to a historic severe drought that occurred soon after grasses were seeded. Different strategies and tactics will be required to manage yellow starthistle in the Southwestern USA than have been previously applied in other areas.

Humans in Changing Shrubland EcosystemsForeword: Humans in Changing Shrubland Ecosystems

Emerging arid-land research and management approaches are increasingly shaped by the recognition of the fact that humans are an integral part of ecosystems. The thrust to study the coupled natural-human dynamics of such systems1 and the growing awareness of the social-ecological nature of rangeland ecosystems2 are prompting a shift in the way we think about current and future challenges facing shrublands in North America and globally. At the same time, increasing understanding of the multiple ecosystem services (both tangible and intangible) provided to society by natural ecosystems3 is further challenging our collective views of how to adapt to current and projected changes affecting the socioecological landscapes of the American West.

Foreword: Humans in Changing Shrubland Ecosystems

Emerging arid-land research and management approaches are increasingly shaped by the recognition of the fact that humans are an integral part of ecosystems. The thrust to study the coupled natural-human dynamics of such systems1 and the growing awareness of the social-ecological nature of rangeland ecosystems2 are prompting a shift in the way we think about current and future challenges facing shrublands in North America and globally. At the same time, increasing understanding of the multiple ecosystem services (both tangible and intangible) provided to society by natural ecosystems3 is further challenging our collective views of how to adapt to current and projected changes affecting the socioecological landscapes of the American West.