Rozida Mohd Khalid

Pengasingan empat steroid daripada daun paku pakis Adiantum latifolium Lam

Isolation of compounds from methanol extract from the leaves of Adiantum latifolium Lam. had been conducted by using radial chromatography and thin layer chromatography (TLC) techniques. Their structures were elucidated by ultraviolet-visible (UV), infrared (IR), nuclear magnetic resonance (NMR) and mass spectroscopy (MS) and by comparison with the previous literatures. Four steroids compound were isolated namely the Stigmast-4-en-3-one (1), Stigmasta-4,22-dien-3-on (2), Stigmasterol (3) and βSitosterol (4).

Pemencilan sebatian botrien dari Hypoxylon rickii dan pengenalpastian pengekodan gen

10-oxodehydrobotrydial (1) and 4β-Acetoxy-9β,10β,15α-trihydroxyprobotrydial (2) have been isolated from Hypoxylon rickii mycelia extract. Compound 2 is probably an intermediate of botrydial, which is a known sesquiterpene phytotoxin. Cluster Omega and Artemis software analysis suggested that HRT6, a possible botrydial-like gene cluster with four genes shows more than 50% similarities compared to Botrytis cinerea botrydial gene cluster, BcBOT. A comparison between proposed 4β-Acetoxy9β,10β,15αtrihydroxyprobotrydial (2) producing pathway and BcBOT proposed pathway shows compatible function of each gene prediction. Further confirmation using RNA gene knock-outs is on-going.