Ruby Patel

ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF CommiphoraMukul(GUGGUL) EXTRACTS AGAINST HeLa CELL-LINE.

ShivangiMathur. Commiphoramukul found in India, Pakistan and Bangladesh. Guggul gum resin has been used to treat various diseases including cancer, atherosclerosis, rheumatism and obesity. The crude extracts obtained from the resin of Commiphoramukul in different solvents like ethyl acetate, methanol, acetone, and hexane were analyzed for phytochemical study. Secondary metabolites steroids, quinines, gum and mucilage, fixed oil and fats were found in all the four different extracts of Commiphoramukul however, oxalate and alkaloids were not found in any extract. Ethyl acetate, methanol, acetone, and hexane extracts from method-1(soxhlet) and method2 (cold) were tested for their ability to scavenge DPPH radical. Ethyl acetate extracts exhibited higher antioxidant activity (45.56%) compared to other solvent extracts. Various concentration of Commiphoramukul plant resin extract were used for MTT assay and highest % inhibition/Cytotoxicity (75.68 %) was found by methanol soxhlet extract having lowest IC50 value (58.5μg/ml).