Rudi Irawan

Sensitivity?stability-optimized surface plasmon resonance sensing with double metal layers

A surface plasmon resonance (SPR) sensor, with a double-metal-layer (gold–silver) configuration that utilizes the sensitivity of the silver and the stability of the gold, was studied experimentally. Both theoretical simulations and experimental results are presented for this configuration. The measured FWHM of the reflectivity curve for the bimetallic-layer configuration is 4.8 times narrower than the single gold film configuration and its stability is better than that of the silver film configuration as well.

Compact fluorescence detection using in-fiber microchannels—its potential for lab-on-a-chip applications

This paper reports a compact and practical fluorescence sensor using an in-fiber microchannel. A blue LED, a multimode PMMA or silica fiber and a mini-PMT were used as an excitation source, a light guide and a fluorescence detector, respectively. Microfluidic channels of 100 microm width and 210 microm depth were fabricated in the optical fibers using a direct-write CO(2) laser system. The experimental results show that the sensor has high sensitivity, able to detect 0.005 microg L(-1) of fluorescein in the PBS solution, and the results are reproducible. The results also show that the silica fiber sensor has better sensitivity than that of the PMMA fiber sensor. This could be due to the fouling effect of the frosty layer formed at the microchannel made within the PMMA fiber. It is believed that this fiber sensor has the potential to be integrated into microfluidic chips for lab-on-a-chip applications.

Two-layered metallic film-induced surface plasmon polariton for fluorescence emission enhancement in on-chip waveguide

We demonstrate an enhancement of fluorescence emission due to bimetallic silver-gold film-induced surface plasmon wave extension. Rhodamine B (RhB) dyes were excited by the evanescent wave field produced from surface plasmon polaritons excited on metal-deposited sections along an embedded strip waveguide. Various silver-gold combinations were used to quantify for the evanescent field enhancement. The underlying silver yields better evanescent field enhancement, while the overlying gold ensures that the stability of the sensing surface is not compromised. In comparison to the conventional single gold film surface plasmon resonance (SPR) configuration, the two-layered metallic structure is capable of enhancing the surface plasmon polariton (SPP) evanescent field considerably, as verified experimentally by the ca. 4.0 times improvement in the RhB fluorescence emission. The compact waveguide structure and improved electric field probing depth can potentially be exploited for on-chip SPR--fluorescence excitation of less concentrated fluorophore-labelled biological and chemical analytes, with a capability of massively parallel processing for high throughput screening.

Rapid constructions of microstructures for optical fiber sensors using a commercial CO2 laser system.

Exposing an optical fiber core to the measurand surrounding the fiber is often used to enhance the sensitivity of an optical fiber sensor. This paper reports on the rapid fabrication of microstructures in an optical fiber using a CO2 laser system which help exposing the optical fiber core to the measurand. The direct-write CO2 laser system used is originally designed for engraving the polymeric material. Fabrications of microstructures such as in-fiber microhole, D-shaped fiber, in-fiber microchannel, side-sliced fiber and tapered fiber were attempted. The microstructures in the fibers were examined using a SEM and an optical microscope. Quality of microstructures shown by the SEM images and promising results from fluorescence sensor tests using in-fiber microchannels of 100μm width, 210μm depth and 10mm length show the prospect of this method for use in optical fiber sensor development. The direct-write CO2 laser system is a flexible and fast machining tool for fabricating microstructures in an optical fiber, and can possibly be a replacement of the time consuming chemical etching and polishing methods used for microstructure fabrications of optical the fiber sensors reported in other literatures.

Detection of Fluorescence Generated in Microfluidic Channel Using In-Fiber Grooves and In-Fiber Microchannel Sensors

In life sciences, the problem of very small volume of sample, analytes, and reagents is often faced. Micro-fluidic technology is ideal for handling costly and difficult-to-obtain samples, analytes, and reagents, because it requires very small volume of samples, in order of microL or even nL. Among many types of optical techniques commonly used for biosensing in microfluidic chip, fluorescence detection technique is the most common. The standard free-space detection techniques used to detect fluorescence emission from microfluidic channel often suffer issues like scattering noise, crosstalks, misalignment, autofluorescence of substrate, and low collection efficiency. This chapter describes two fluorescence detection methods, based on in-fiber microchannels and in-fiber grooves, which can solve those problems, as the techniques integrate the excitation and emission light paths, and the sensing part. Utilizing an optical fiber as a sensing component makes these detection techniques suitable for lab-on-a-chip or microTAS applications.

Surface plasma resonance biosensor based on prism geometry

We demonstrated the improvements of an optical biosensor system using a long range surface plasma resonance technique. As preliminary simulation results, we present the prism-based biosensor system with a broader operating range to improve the sensitivity for a wider the measurable reflective index range and a narrower absorbing peak. This proposed optical biosensor system could be used to implement a compact immunoassay device.

Modeling Fluorescence Detection for Microfluidic Card

Simple fluorescence detection system and disposable microfluidic card have been developed and tested. A blue LED, optical fiber, CCD detector, Mylar and PMMA, and fluorescein and BSA-FITC were used as an excitation source, waveguide, detector, microfluidic substrate, and sample, respectively. The results show the system can detect 0.01–1000 ng/ml of fluorescein solutions with linear response. A technique to avoid cross-talks between the adjacent channels in a multi-channel microfluidic card is also addressed. The test using BSA as a model analyte demonstrates its feasibility for on-chip biosensor applications. This prototype can be used as a platform to develop a compact bio-fluorescence sensor for microfluidic analysis systems.

Compact fluorescence detection using in-fiber microchannels

Applications involving fluorescence detection in point-of-care systems are both interesting and challenging in nature. The applications usually require a simple, compact, robust, highly sensitive yet affordable system. As a result, the system needs to be efficient in fluorescence detection by using practical and easily fabricated, hence inexpensive sensors. In this paper, a fluorescence sensor using an in-fiber microchannel has been developed and tested successfully. A blue LED, multimode PMMA or silica fiber, mini-PMT and fluorescein in PBS pH 7.4 buffer solution were used as the excitation source, light guide, fluorescence detector and sample, respectively. Microfluidic channels of 100μm width and 1cm length were fabricated in the optical fibers using a direct write CO2 laser system. The channels in the fibers were examined using a SEM and an optical microscope. Experimental results show that the sensor is highly sensitive, being able to detect 0.1 μg/L of fluorescein in the PBS buffer solution, with good signal to noise ratio and the results are reproducible. The data obtained using silica fibers as sensors when compared with the results from PMMA fibers show that the silica fiber sensor has better sensitivity than the PMMA fiber sensor. This could be due to the fouling effect created by the frosty layer at the bottom of the microchannel made within the PMMA fiber. Our future work will integrate the fiber sensor into microfluidic chips for lab-on-a-chip applications.