Rudolf Gruber

Quality of life and family functioning in children with nonsyndromic orofacial clefts at preschool ages.

Children with orofacial clefts (OFC) at preschool ages may have to tolerate psychosocial disadvantages due to their altered speech and facial appearance probably affecting their quality of life (QoL) and family functioning. In 147 children with OFC aged between 5 and 6 years and their families, the QoL and family functioning were analyzed using the KINDL questionnaire for measuring health-related QoL in children and impact on family scale. The KINDL scores were lowest in the dimension self-esteem. In all dimensions, the KINDL scores of children were higher than those of the parents suggesting a superior QoL than the caregivers estimated (P < 0.001). In affected families, the impact on family scale dimensions personal impact and impact on coping strategies were found highest. Families having children with isolated cleft lip or cleft lip and palate had higher impacts on coping strategies when compared with children having isolated cleft palate (P < 0.041). The impact for siblings (P < 0.02) was found highest in patients with cleft lip and palate. In all examined dimensions, children with OFC perceived a higher QoL than their caregivers expected. However, self-esteem seems to be problematic in all types of OFC and in both genders. Knowledge of potential impacts related to the type of cleft and the gender of the patient will probably facilitate health care professionals to identify children and families at high risk to experience a reduced QoL and may help to offer specific support and treatment strategies. Abbreviations: OFC, orofacial cleft; CLP, cleft lip and palate; CL, isolated cleft lip (and alveolus); CP, isolated cleft palate; QoL, quality of life; IFS, impact on family scale

Sinus floor augmentation with recombinant human growth and differentiation factor-5 (rhGDF-5): a pilot study in the Goettingen miniature pig comparing autogenous bone and rhGDF-5

AIM The aim of this study was to test the hypothesis that recombinant human growth and differentiation factor-5 (rhGDF-5) in combination with a beta-tricalcium phosphate (beta-TCP) scaffold material results in superior bone formation in sinus floor augmentations in miniature pigs compared with a particulated autogenous bone graft combined with the scaffold material. MATERIAL AND METHODS Six adult female Goettingen minipigs underwent a maxillary sinus floor augmentation procedure. In a split-mouth design, the sinus floors were augmented with beta-TCP mixed with autogenous cortical bone chips, in a ratio of approximately 1 : 1, on one side. The contralateral test site was augmented using beta-TCP coated with two concentrations of rhGDF-5 (400 microg rhGDF-5/g beta-TCP or 800 microg rhGDF-5/g beta-TCP; three animals in each case). Simultaneously, one dental implant was inserted into each sinus floor augmentation. After 12 weeks, a histological and histomorphometric assessment of non-decalcified histological specimens was made. RESULTS There were significantly higher mean values of volume density of newly formed bone using beta-TCP coated with two concentrations of rhGDF-5 (400 microg: 32.9%; 800 microg: 23.9%) than with the corresponding control (autogenous bone/beta-TCP) (14.6%, 12.9%) (P=0.012, P=0.049). The bone-to-implant contact rates (BIC) were significantly enhanced in test sites (400 microg: 84.2%; 800 microg: 69.8%) compared with the corresponding control sites (24.8%, 40.8%) (P=.027, P=.045). CONCLUSION rhGDF-5 delivered on beta-TCP significantly enhanced bone formation compared with beta-TCP combined with autogenous bone in sinus lift procedures in miniature pigs.

Sinus floor augmentation with recombinant human growth and differentiation factor‐5 (rhGDF‐5): a histological and histomorphometric study in the Goettingen miniature pig

AIM The aim of this study was to test the hypothesis that recombinant human growth and differentiation factor-5 (rhGDF-5) enhances bone formation in sinus floor augmentations in miniature pigs. MATERIAL AND METHODS The maxillary sinus floors in 12 adult female Goettingen minipigs were augmented with beta-tricalcium phosphate (beta-TCP) on one side. The contralateral test side was augmented using two concentrations of rhGDF-5 (400 microg rhGDF-5/g beta-TCP; 800 microg rhGDF-5/g beta-TCP) delivered on beta-TCP (six animals each). One dental implant was inserted into each sinus floor augmentation. After 4 and 12 weeks, histological and histomorphometric assessment of non-decalcified histological specimens was performed. RESULTS The results showed significantly higher mean values of volume density (VD) of newly formed bone using the concentration of 400 microg/g beta-TCP (22.8%) compared with the respective control (8%) after 4 weeks (P=0.05). The bone-to-implant contact rates were also significantly enhanced after 4 weeks between test sites (400 microg: 41.9%; 800 microg: 40.6%) and control sites (400 microg: 7.8%; 800 microg: 16.4%) (400 microg: P=0.024; 800 microg: P=0.048). CONCLUSION It is concluded that rhGDF-5 delivered on beta-TCP significantly enhanced early bone formation compared with beta-TCP alone in sinus lift procedures in miniature pigs.

CPA6, FMO2, LGI1, SIAT1 and TNC are differentially expressed in early- and late-stage oral squamous cell carcinoma - A pilot study

To identify novel genes that could be involved in oncogenesis of oral squamous cell carcinoma a microarray-based gene-expression analysis was performed using tumour samples from patients with low-stage (n=4) and high-stage (n=4) disease in a pilot study. Genes (601) were found to be significantly regulated in cancer tissue compared to adjacent intraindividual mucosa controls. Genes (25) were identified with differences in their regulation comparing samples from early-stage cancer with those from advanced disease. The gene expression pattern of 5 of 7 genes examined by real-time-PCR verified the results received from the microarray-experiment. Among these, FMO2, CPA6, TNC and SIAT1 were significantly upregulated in early disease stages. LGI1 gene expression was significantly enhanced in normal adjacent mucosa of patients with early-stage disease without showing a differential expression in carcinoma biopsies. With this pilot study several novel genes were identified, which could be related to early and late stage disease. Hypotheses from these findings are discussed and have to be confirmed in a larger study sample.

Initial forces generated by three types of thermoplastic appliances on an upper central incisor during tipping.

The force properties of thermoformed appliances have not been systematically investigated. Therefore, the aim of the present study was to quantify the forces delivered by thermoplastic appliances manufactured from three different materials, with the same thickness, on a central upper incisor, during tipping. Five identical appliances were manufactured from three different materials all with a thickness of 1.0 mm (Ideal Clear, Erkodur, and Biolon). For measuring the forces, an isolated measuring tooth, as part of a standardized resin model incorporated in a newly developed measuring device, was tipped in nine 2.7 arc minute (0.04629 degree) steps, from 0 to 0.416 degrees in the vestibular and palatal directions around a rotational axis through the virtual apex, after positioning an appliance on the model. For statistical analysis, the force components Fx/tipping and Fz/intrusion at a displacement of +/-0.151 mm from the incisor edge were determined. Means and standard deviations (SDs) were calculated. The Kruskal-Wallis test for overall effects and the Wilcoxon two-sample test for individual group pairings were used (P < 0.05 significance level). The mean Fx forces ranged from -2.82 N (SD 0.62) to 5.42 N (SD 0.56). The mean Fz forces were between -0.14 N (SD 0.52) and -2.3 N (SD 0.43). The highest intrusive forces were measured during vestibular displacement of the measuring tooth. The forces delivered by the Biolon appliance were found to be much greater (P < 0.01) than those of the other materials. The forces delivered by the materials investigated were mostly higher than those stated in the literature.

Optimizing preparation design for metal-free composite resin crowns

STATEMENT OF PROBLEM Although composite resin materials are used for posterior crown restorations, the influence of preparation design, material thickness, convergence angle, and method of cementation on fracture resistance remains unclear. PURPOSE The purpose of this in vitro study was to test the hypothesis that minimal preparation designs provide an acceptable level of fracture resistance for posterior composite resin crowns. MATERIAL AND METHODS Nonreinforced Artglass composite resin crowns (n=128) were fabricated on human molars in 16 test groups (n=8). Axial tooth preparation included a 1-mm-deep shoulder or a 0.5-mm chamfer preparation, whereas occlusal reduction was either 0.5 mm or 1.3 mm. The total angle of convergence was 4 or 11 degrees and the crowns were cemented either with glass ionomer cement (Ketac Cem) or resin cement (2bond2). After 10,000 thermal cycles, crowns were vertically loaded until failure occurred; load was measured in newtons. Statistical analysis was performed by a 4-way ANOVA (alpha=.05). RESULTS Statistical analysis revealed significant effects of occlusal thickness (P<.001), cement (P<.001), preparation design (P=.011), and convergence angle (P=.001) on the fracture resistance of the composite crowns. For composite resin crowns with an occlusal thickness of 0.5 mm, the resistance to fracture was lower than for crowns with a 1.3-mm thickness. Fracture resistance was greater when resin cement was used, and greater for the chamfer finish line than for the shoulder finish line. Use of a greater total convergence angle reduced fracture resistance. CONCLUSIONS Fracture resistance of composite resin crowns was significantly improved by increasing the occlusal thickness of the crowns, by using resin cement, and by reducing the total convergence angle.

Detection and size differentiation of simulated tooth root defects using flat-panel volume computerized tomography (fpVCT)

OBJECTIVE Our aim was to evaluate the capacity of flat-panel volume computerized tomography (fpVCT) to enable the observer to detect and differentiate 3 different sizes of simulated tooth root defects in radiographs. STUDY DESIGN Three simulated tooth root defects of different sizes and a defect-free area (160 sites in total) were randomly distributed on the buccal and lingual surface of 20 mandibular premolar roots of Sus scrofa domestica with round burs. For the imaging of the specimens, an fpVCT prototype was used. Findings were evaluated by 3 observers. RESULTS Cavity 0 (no lesion) was correctly identified in 53%, cavity 1 in 69%, cavity 2 in 96%, and cavity 3 in 89%. Altogether, the simulated cavities were classified in a correct manner in 77%. The values were compared using receiver operating characteristic curves. The area under the curve (AUC) for cavity 0 versus the pooled results for cavities 1-3 was found to be 0.72. The AUC for the pooled results for 0-2 (no pathologic impact) versus cavity 3 (potential pathologic impact) was 0.94. There was no significant dependence of the results on the observer (P = .37). Results with P < .05 were considered to be significant. CONCLUSIONS Flat-panel volume computerized tomography, which is currently used only as a research tool, has a high potential in detection and differentiation at an early stage of external root resorption cavities with pathologic relevance..

Ectopic bone formation after implantation of a slow release system of polylactid acid and rhBMP‐2

OBJECTIVES The present study was conducted to test the hypothesis that preshaped polylactic acid (PLA) implants loaded with recombinant human bone morphogenic protein 2 (rhBMP-2) can induce bone formation in a rat ectopic model. MATERIALS AND METHODS Two groups of porous cylindrical poly-DL-lactic acid implants of 8-mm diameter were produced by gas foaming with CO(2), incorporating 48 and 96 microg rhBMP-2, respectively, into each implant. Blank PLA implants were used as controls. The release of BMPs and the induction of alkaline phosphatase were assessed in vitro. Osteoinduction in vivo was tested by insertion of 15 implants from each group into the gluteal muscles of Wistar rats. Five implants from each group were retrieved after 6, 13 and 26 weeks and assessed using flat panel volume detector computed tomography and light microscopy. RESULTS Both groups of implants showed increased release of rhBMP-2 during the first 24-48 h, with a slightly higher amount being released from the implants with 48 microg. Release during subsequent intervals was <100 ng/72 h in the low-concentration group and >100 ng in the group with 96 microg rhBMP-2. Implants with 95 microg rhBMP-2 exhibited bone formation in vivo on the outside of the implants across the observation period of 26 weeks with invasion of bone into the pores, whereas implants with 48 microg rhBMP-2 failed to induce the formation of bone tissue. No bone formation was found in the control implants. CONCLUSIONS The results suggest that release rates of rhBMP-2 for ectopic bone induction have to be >100 ng/72 h to maintain the osteoinductive activity of the tested porous PLA implants. This slow release system may have impact on alveolar bone augmentation procedures when used as individually preformed osteoinductive implants.

Measurement of the midpalatal suture width.

OBJECTIVE To conduct a pilot study to investigate the potentiality to determine the midpalatal sutural width radiographically with a flat-panel volume computed tomography (fpVCT) in a porcine model. MATERIALS AND METHODS Bone samples from the midpalatal suture of five young (16 weeks) and five old (200 weeks) sus scrofa domestica were gathered. The midpalatal suture width was measured via fpVCT and compared to respective histological preparations. Results with P < .05 were considered significant. RESULTS The data obtained by fpVCT and by histomorphometric analysis reveal a highly significant age dependency of the measured suture width (both P < .0001), with lower suture width values in older subjects compared to the younger group. The averaged suture widths measured in the fpVCT images shows a distinctively higher mean compared to the histomorphometric data with high statistical significance (P < .0001). The evaluated difference between both methods was almost constant. CONCLUSION fpVCT is a powerful tool for determining midpalatal sutural width.

Imaging of the midpalatal suture in a porcine model: Flat-panel volume computed tomography compared with multislice computed tomography

OBJECTIVE An investigation was conducted to compare the image quality of prototype flat-panel volume computed tomography (fpVCT) and multislice computed tomography (MSCT) of suture structures. STUDY DESIGN Bone samples were taken from the midpalatal suture of 5 young (16 weeks) and 5 old (200 weeks) Sus scrofa domestica and fixed in formalin solution. An fpVCT prototype and an MSCT were used to obtain images of the specimens. The facial reformations were assessed by 4 observers using a 1 (excellent) to 5 (poor) rating scale for the weighted criteria visualization of the suture structure. A linear mixed model was used for statistical analysis. Results with P < .05 were considered to be statistically significant. RESULTS The visualization of the suture of young specimens was significantly better than that of older animals (P < .001). The visualization of the suture with fpVCT was significantly better than that with MSCT (P < .001). CONCLUSION Compared with MSCT, fpVCT produces superior results in the visualization of the midpalatal suture in a Sus scrofa domestica model.