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Dive into the research topics where Rudolphe Serreyn is active.

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Featured researches published by Rudolphe Serreyn.


Journal of Chromatography B | 2003

Quantitation of soy-derived phytoestrogens in human breast tissue and biological fluids by high-performance liquid chromatography.

Julie Maubach; Marc Bracke; Arne Heyerick; Herman Depypere; Rudolphe Serreyn; Marc Mareel; Denis De Keukeleire

A new and reliable HPLC method for the quantitation of daidzein, equol, and genistein in human breast tissue has been developed. The method was applied to biopsies from women undergoing breast reductions, who, prior to surgery, had ingested either a soy isoflavone preparation or a placebo tablet. The results were compared with data collected for urine and serum of the same subjects using standard methods. The limits of detection in the breast tissue homogenate were 24.7 nmol/l for daidzein, 148.0 nmol/l for equol, and 28.4 nmol/l for genistein (S/N of 3). The chromatographic limits of quantitation were 62.5 nmol/l for daidzein and genistein, and 125.0 nmol/l for equol, for which the accuracies were 86.0%, 83.6%, and 81.8%, respectively. The coefficients of variation of these measurements were all below 20% (11.1% for daidzein, 16.4% for genistein, and 13.2% for equol). The sample preparation comprised a concentration step and the absolute limits of quantitation were, therefore, 4.7 nmol/l, 18.8 nmol/l, and 0.94 nmol/l for daidzein and genistein, and 9.4 nmol/l, 37.5 nmol/l, and 1.9 nmol/l for equol in urine, serum, and breast tissue homogenate, respectively. Recoveries were between 70% (+/-5.6%) in breast tissue homogenate and 100% (+/-14.1%) in urine and serum for all three compounds. Equol (less than 1 micromol/l homogenate) was found to be the predominant phytoestrogen in breast tissue and its concentrations exceeded those in serum. The concentrations of phytoestrogens were at least 100-fold higher in urine than in serum and breast tissue.


European Journal of Cell Biology | 2001

8-Prenylnaringenin, the phytoestrogen in hops and beer, upregulates the function of the E-cadherin/catenin complex in human mammary carcinoma cells.

Haojing Rong; Tom Boterberg; Julie Maubach; Christophe P. Stove; Herman Depypere; Séverine Van Slambrouck; Rudolphe Serreyn; Denis De Keukeleire; Marcus Mareel; Marc Bracke

The E-cadherin/catenin complex is a powerful invasion suppressor in epithelial cells. It is expressed in the human MCF-7 breast cancer cell line family, but functionally defective in the invasive MCF-7/6 variant. Previous experiments have shown that IGF-I, tamoxifen, retinoic acid and tangeretin are able to upregulate the function of this complex in MCF-7/6 cells. We investigated the effect of 8-prenylnaringenin (8-PN), the phytoestrogen present in hops and beer, on aggregation, growth and invasion in MCF-7/6 cells. 8-PN was found to stimulate E-cadherin-dependent aggregation and growth of MCF-7/6 cells in suspension. These effects could be inhibited by the pure anti-estrogen ICI 182,780. 8-PN did not affect invasion of MCF-7/6 cells in the chick heart assay in vitro. In all these aspects 8-PN mimics the effects of 17beta-estradiol on MCF-7/6 cells.


Annals of Oncology | 1998

Abdominal pain with anorexia in patients with breast carcinoma

Ph. Van Trappen; Rudolphe Serreyn; A. E. Elewaut; Veronique Cocquyt; S. Van Belle

Breast cancer is the second commonest primary tumour responsible for gastrointestinal metastases after malignant melanoma. The real incidence of gastrointestinal metastases in breast cancer patients is probably underestimated owing to the non-specific presenting symptoms and death of patients caused by other more obvious metastases. The predominant histological subtype of gastrointestinal metastases of breast cancer is invasive lobular carcinoma and the median interval from diagnosis of primary breast cancer to gastrointestinal metastases is five years. We report two cases of disseminated breast cancer with gastrointestinal involvement with a rather long survival.


The Breast | 2003

Adenoid cystic carcinoma of the breast in a 19-year-old girl

S Delanote; R. Van den Broecke; V.R.J Schelfhout; Rudolphe Serreyn

A case of adenoid cystic carcinoma of the breast in a 19-year-old girl is presented. As this tumor has specific characteristics and diagnostic criteria, this case illustrates the importance of an accurate histological diagnosis.


Clinical Cancer Research | 2007

Effect of Tibolone on Breast Cancer Cell Proliferation in Postmenopausal ER+ Patients: Results from STEM Trial

E. Kubista; Juan V.M. Planellas Gomez; Mitch Dowsett; Jean-Michel Foidart; Kamil Pohlodek; Rudolphe Serreyn; Michail Nechushkin; Alexey Manikhas; Victor F. Semiglazov; Cornelius C.M. Hageluken; Christian F. Singer

Purpose: Tibolone is a selective tissue estrogenic activity regulator, approved for the treatment of vasomotor symptoms in postmenopausal women. We have done an exploratory, double-blind, randomized, placebo-controlled pilot trial to investigate the tissue-specific effects of 2.5 mg tibolone on breast cancer in postmenopausal women, in particular on tissue proliferation (STEM, Study of Tibolone Effects on Mamma carcinoma tissue). Experimental Design: Postmenopausal women with initially stage I/II, estrogen receptor–positive (ER+) primary breast cancer, were randomly assigned to 14 days of placebo or 2.5 mg/d tibolone. Core biopsies of the primary tumor were obtained before and after treatment. Ki-67 and apoptosis index were analyzed in baseline and corresponding posttreatment specimen. Results: Of 102 enrolled patients, 95 had evaluable data. Baseline characteristics were comparable between both treatment groups. Breast cancer cases are mainly invasive (99%), stage I or II (42% and 50% respectively), and ER+ (99%). Median intratumoral Ki-67 expression at baseline was 13.0% in the tibolone group and 17.8% in the placebo group, and decreased to 12.0% after 14 days of tibolone while increasing to 19.0% in the placebo group. This change from baseline was not significantly different between tibolone and placebo (Wilcoxon test; P = 0.17). A significant difference was observed between the treatment groups when the median change from baseline apoptosis index was compared between the treatment groups (tibolone, 0.0%; placebo, +0.3%; Wilcoxon test; P = 0.031). The incidence of adverse effects was comparable. Conclusions: In ER+ breast tumors, 2.5 mg/d tibolone given for 14 days has no significant effect on tumor cell proliferation.


Medical Oncology | 2003

The role of biological markers as predictors of response to preoperative chemotherapy in large primary breast cancer.

Veronique Cocquyt; Vera R. Schelfhout; Phillip Blondeel; Herman Depypere; Kristof K. Daems; Rudolphe Serreyn; Marleen Praet; Simon J. P. Van Beele

The aim of this prospective study was to evaluate biological markers, their correlation with response and outcome, and the change in these markers under the influence of preoperative chemotherapy (PCT) in patients with a large primary breast cancer.One hundred and thirty-five women were treated with PCT, followed by locoregional therapy and adjuvant treatment. Estrogen receptor (ER), progesterone receptor (PgR), HER-2, p53, and cathepsin D were determined by immunohistochemistry (IHC) before and after PCT. The overall response (OR) was 70% and the pathologic complete response (pCR) was 13%. Forty-four percent of the patients could be offered breast-conserving surgery (BCS). At a median follow-up of 50 mo the overall survival is 82% and the disease-free survival is 70%. No local recurrence (LR) has developed following BCS.Invasive ductal carcinoma (IDC) was more frequently ER-negative and HER-2-positive than invasive lobular carcinoma (ILC).P53-negative and ER-negative patients seemed to be more chemosensitive compared to p53-positive patients (74% vs 53%) and ER-positive patients (75% vs 65%), but this difference did not reach statistical significance. A trend toward higher complete pathologic remission rate was seen for ER-negative patients (p=0.0609). PgR, HER-2, and cathepsin D were not related to response.The pattern of biological markers did not change with PCT, making repeated determination useless.


European Journal of Nuclear Medicine and Molecular Imaging | 2000

Technetium-99m RP527, a GRP analogue for visualisation of GRP receptor-expressing malignancies : a feasibility study

Christophe Van de Wiele; Filip Dumont; Rudi Vanden Broecke; Wim Oosterlinck; Veronique Cocquyt; Rudolphe Serreyn; Susan H. Peers; J. R. Thornback; Guido Slegers; Rudi Dierckx


Ejso | 2003

Different responses to preoperative chemotherapy for invasive lobular and invasive ductal breast carcinoma

Veronique Cocquyt; Phillip Blondeel; Herman Depypere; Marleen Praet; V.R.J Schelfhout; O.E. Silva; J. Hurley; Rudolphe Serreyn; Kristof K. Daems; S. Van Belle


Journal of the National Cancer Institute | 1999

Influence of Tangeretin on Tamoxifen's Therapeutic Benefit in Mammary Cancer

Marc Bracke; Herman Depypere; Tom Boterberg; Veerle Van Marck; Krist’l M. Vennekens; E. Vanluchene; Margareta Nuytinck; Rudolphe Serreyn; Marc M. Mareel


European Journal of Cell Biology | 1997

Functional downregulation of the E-cadherin/catenin complex leads to loss of contact inhibition of motility and of mitochondrial activity, but not of growth in confluent epithelial cell cultures

Marc Bracke; Herman Depypere; C Labit; Veerle Van Marck; K Vennekens; Sj Vermeulen; I Maelfait; Jan Philippé; Rudolphe Serreyn; Marcus Mareel

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Herman Depypere

Ghent University Hospital

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Marcus Mareel

Ghent University Hospital

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S. Van Belle

Ghent University Hospital

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Tom Boterberg

Ghent University Hospital

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