Rui Fan
Ningbo University
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Featured researches published by Rui Fan.
Stroke | 2015
Liyuan Han; Qunhong Wu; Changyi Wang; Yanhua Hao; Jinshun Zhao; Lina Zhang; Rui Fan; Yanfen Liu; Runhua Li; Zhongwei Chen; Tao Zhang; Sihan Chen; Jianping Ma; Shengyuan Liu; Xiaolin Peng; Shiwei Duan
Background and Purpose— Total homocysteine level (tHcy) is a risk factor of ischemic stroke (IS) and coronary heart disease. However, the results are conflicting and mainly focused on healthy individuals in developed countries. Methods— A prospective, population-based cohort study was conducted among 5935 participants from 60 communities in the city of Shenzhen, China. A Cox regression analysis was applied to evaluate the contribution of tHcy to the risk of IS and coronary heart disease. The effect of folic acid supplementation on tHcy levels was also evaluated among 501 patients with essential hypertension, who received an average of 2.5 years of folic acid supplementation. Results— After adjustment for confounding factors, the hazard ratios (95% confidence intervals) of IS caused by hyperhomocysteinemia were 2.18 (1.65–2.89), 2.40 (1.56–3.67), and 2.73 (1.83–4.08) in the total, male, and female participants, respectively. Compared with normal levels of tHcy (<15 &mgr;mol/L), the hazard ratios (95% confidence intervals) for IS in the highest tHcy category (≥30 &mgr;mol/L) were 4.96 (3.03–8.12), 6.11 (3.44–10.85), and 1.84 (0.52–6.46) in the total, males, and females participants, respectively. However, we did not observe a significant relationship between tHcy and the risk of coronary heart disease. The 2.5 years of folic acid supplementation reduced tHcy levels by 6.7 &mgr;mol/L (27.92%) in patients with essential hypertension. Conclusions— Hyperhomocysteinemia in Chinese hypertensive patients is significantly associated with IS risk but not coronary heart disease susceptibility, and folic acid supplementation can efficiently reduce tHcy levels.
Hypertension Research | 2015
Liyuan Han; Panpan Liu; Changyi Wang; Qi-Long Zhong; Rui Fan; Lin Wang; Shiwei Duan; Lina Zhang
The potential effects of the interactions between DNA methylation (CpG1 and CpG2-5 methylation levels) of the α-adducin (ADD1) gene promoter and ADD1 tagSNPs (tag single-nucleotide polymorphisms) or the environmental factors on essential hypertension (EH) risk have not been clarified. Thus, we performed an age- and gender-matched case–control study to investigate the association between ADD1 tagSNPs and EH. A total of 1020 subjects with EH and 1020 normotensive subjects were genotyped by melting temperature shift technology. Logistic regression was used to assess the associations of ADD1 tagSNPs, environmental factors and EH. The generalized multifactor dimensionality reduction (GMDR) method was applied to explore the potential interactions. Under additive, dominant and recessive models, no significant associations were evidenced between EH and rs3755885, rs2071694, rs4963 or rs3775067 with the complete data set or the gender-stratified analysis after adjusting for triglycerides, body mass index and alcohol consumption. However, we observed a significant association between rs4961 and EH under the dominant model after Bonferroni correction when adjusting for confounding factors in the entire sample (odds ratio (OR)=0.64, 95% confidence interval (CI)=0.50–0.83, P=0.001). In GMDR, the two-factor interaction model of alcohol consumption and DNA methylation (CpG1 methylation) was the best model, with a maximum cross-validation consistency of 9/10 and testing balance accuracy of 0.63 (P=0.01). Our results indicate that the SNP rs4961 has a protective role in the development of EH. In conclusion, the interactions between alcohol consumption and DNA methylation (CpG1 methylation) of the ADD1 gene promoter have a significant role in modifying EH susceptibility.
Molecular Medicine Reports | 2015
Rui Fan; Wei‑Jie Wang; Qi-Long Zhong; Shiwei Duan; Xu‑Ting Xu; Ling‑Mei Hao; Jing Zhao; Lina Zhang
Essential hypertension (EH) is commonly accompanied by a dysfunction of glucose metabolism. Glucokinase (GCK) is a key enzyme involved in glucose metabolism. The aim of the present study was to investigate whether GCK gene-body methylation contributed to the risk of EH. A total of 47 patients with EH and 47 age-matched controls were recruited for methylation research in the current study. GCK gene-body methylation was measured using bisulphite pyrosequencing technology. DNA methylation levels were closely correlated among CpG1, CpG2 and CpG3 (r>0.70; P<0.001), in contrast with a weaker correlation between CpG4 and the preceding three CpGs (r<0.3 or r=1; P>0.05). Significantly lower CpG13 methylation (cases vs. controls, 49.13 ± 5.72 vs. 53.49 ± 7.53%; adjusted P=0.006) and significantly higher CpG4 methylation (cases vs. controls, 46.34 ± 6.48 vs. 34.74 ± 12.73%; adjusted P=0.002) were observed in patients with EH. The present study indicated that aberrant methylation of the GCK gene body was significantly associated with the risk of EH in the population assessed. The discrepancies between CpG1‑3 and CpG4 methylation may suggest distinct roles for each of them in the determination of the risk of EH.
Cytogenetic and Genome Research | 2015
Rui Fan; Shuqi Mao; Fade Zhong; Minli Gong; Fengying Yin; Lingmei Hao; Lina Zhang
The purpose of the present study was to investigate whether methylation of the angiotensin II type 1 receptor (AGTR1) promoter contributed to the risk of essential hypertension (EH). A total of 96 EH cases and 96 gender- and age-matched healthy controls were recruited. Methylation of 8 CpG dinucleotides (CpG1-8) in the AGTR1 promoter was examined using the bisulphite pyrosequencing technology. Three CpG dinucleotides (CpG6-8) could not be well sequenced, therefore only the remaining 5 CpG sites were analysed. A significantly lower CpG1 methylation level was identified in EH cases than in controls (cases vs. controls: 6.74 ± 4.32% vs. 9.66 ± 5.45%, p = 0.007), and no significant association was observed in the remaining analyses. In addition, significantly lower CpG1 (p = 0.028) and higher CpG2 (p = 0.032) methylation levels were observed in males than in females. In the breakdown association test by gender, a higher CpG1 methylation level was also identified in EH in both males (p = 0.034) and females (p = 0.020). Receiver operating characteristic curves showed that CpG1 methylation was a significant predictor of EH. Furthermore, CpG1 methylation was inversely correlated with uric acid levels in controls. The present study suggests that CpG1 hypomethylation in the AGTR1 promoter is likely associated with the risk of EH in the population assessed.
Clinical and Experimental Hypertension | 2015
Changyi Wang; Liyuan Han; Qunhong Wu; Renjie Zhuo; Kui Liu; Jinshun Zhao; Lina Zhang; Yanhua Hao; Rui Fan; Yanfen Liu; Runhua Li; Zhongwei Chen; Tao Zhang; Sihan Chen; Jianping Ma; Shengyuan Liu; Xiaolin Peng; Shiwei Duan
Abstract Background: To assess the association between total plasma homocysteine (tHcy) and ischemic stroke (IS) in hypertensive subjects in a matched case-control study. Methods: This is a 1:2 matched and population-based case-control study, all of the participants were recruited from the 60 communities in Shenzhen, China. Demographic and socioeconomic characteristics, medical records, lifestyle risk factors and other clinical characteristics were obtained from all of the subjects. The association between tHcy and incidence of IS was analyzed by using conditional logistic regression models. Results: The median values of plasma tHcy were significantly higher in IS subjects than in non-IS subjects, especially in women. After adjusted for the confounding factors in Model 2, compared with the lowest quartile of tHcy, the odds ratios (ORs) and 95% CIs of the highest quartile of tHcy for IS were 0.83 (0.36–1.90) in men, 4.51 (1.29–15.7) in women and 1.31 (0.70–2.47) in the total subjects; the ORs and 95% CIs for IS per 5 μmol/L increase in homocysteine were 1.11 (0.99–1.22), 1.25 (1.03–1.58) and 1.15 (1.01–1.28) in men, women and total subjects, respectively. We observed significant associations in crude model, Model 1 and Model 2 in women for the comparison of tHcy ≥ 15 μmol/L versus < 15 μmol/L. Interaction analysis showed that the association of tHcy with IS was significant in women (p-interaction = 0.04). Conclusion: This matched case-control study indicates that tHcy may increase the susceptibility to IS in essential hypertension subjects, especially in women. Further large prospective cohort studies are needed to confirm our findings.
Asia Pacific Journal of Clinical Nutrition | 2015
Changyi Wang; Qunhong Wu; Lina Zhang; Yanhua Hao; Rui Fan; Xiaolin Peng; Shengyuan Liu; Zhongwei Chen; Tao Zhang; Sihan Chen; Jianping Ma; Shiwei Duan; Liyuan Han
BACKGROUND AND OBJECTIVES There is only limited available evidence of a relationship between total plasma homocysteine (tHcy) levels and type 2 diabetes in hypertensive subjects. METHODS AND STUDY DESIGN A total of 5,935 Chinese essentially hypertensive subjects were recruited by cluster sampling from 60 communities. The cases had diabetes, whereas the controls did not. Anthropometric indices and biochemical parameters were assessed using standard procedures. A multivariable analysis was performed to analyze the association of tHcy and type 2 diabetes susceptibility in hypertensive subjects. RESULTS The 5,241 controls (women/men: 2,716/2,625) and 594 cases (women/men: 291/303) were recruited consecutively. The level of tHcy was dose- dependently associated with type 2 diabetes in the hypertensive women subjects. After controlling for corresponding confounding factors, a significant trend was only noted in the women subjects, with odds ratios per 5 μmol/L tHcy of 1.11 (95% confidence interval (CI), 1.07-1.16) in the crude model, 1.05 (95% CI, 1.01-1.11) in model 1, and 1.07 (95% CI, 1.02-1.13) in model 2. However, no significant result was found for levels of tHcy>or=15 μmol/L vs <15 μmol/L in the men, women and all hypertensive subjects. CONCLUSIONS When the level of tHcy was divided into quartiles, tHcy was positively associated with type 2 diabetes in hypertensive women subjects. However, when the level of tHcy was separated into hyperhomocysteinemic (>or=15 μmol/L) and normal (<15 μmol/L), no significant results were observed.
Journal of Hypertension | 2013
Panpan Liu; Qi-Long Zhong; Fang Yuan; Fade Zhong; Rui Fan; Li-Juan Fei; Lingmei Hao; Xu-Jun Qiu; Shiwei Duan; Lina Zhang
Background: The ADD1 Gly460Trp polymorphism has been linked to essential hypertension (EH) in multiple populations, but the results were inconsistent. The goal of our study is to investigate the contribution of ADD1 Gly460Trp polymorphism and environmental factors to the risk of EH. Methods: We conducted a case-control study including 1020 hypertensive cases and 1020 controls, and the gender and age were well matched between hypertensive and control groups. Blood samples and participants information were also collected. Using the melting temperature shift technology, the ADD1 Gly460Trp polymorphism was genotyped among all subjects. Multifactor dimensionality reduction (MDR) was used to identify the interactions among the ADD1 Gly460Trp polymorphism and the nongenetic factors. Results: Our results showed that body mass index (BMI), total cholesterol, triglycerides, and drinking were significantly associated with EH (P<0.05). In addition, the Gly460Trp polymorphism was found significantly associated with hypertension at allelic level (P<0.01; OR=0.85; 95%CI=0.75-0.96). A breakdown association analysis by gender showed the Gly460Trp polymorphism was associated with EH only in female (P<0.01; OR=0.79; 95%CI=0.68- 0.92). MDR analysis indicated that there was an interaction among BMI, high density lipoprotein, drinking, and rs4961 involved in the risk of EH. Conclusion: The present study indicated the Gly460Trp polymorphism was associated with EH in female Han Chinese, which might contribute to EH via interactions with non-genetic factors.
BioMed Research International | 2016
Tianlun Gu; Shuqi Mao; Rui Fan; Fade Zhong; Fubao Zhu; Lingmei Hao; Lina Zhang; Fengying Yin
Aldosterone synthase (CYP11B2) is closely linked to essential hypertension (EH). However, it remains unclear whether the methylation of the CYP11B2 promoter is involved in the development of EH in humans. Our study is aimed at evaluating the contribution of CYP11B2 promoter methylation to the risk of EH. Methylation levels were measured using pyrosequencing technology in 192 participants in a hospital-based case-control study. Logistic regression and multiple linear regression analyses were utilized to adjust for confounding factors and the GMDR method was applied to investigate high-order gene-environment interactions. Although no significant result was observed linking the four analyzed CpG sites to EH, GMDR detected significant interactions among CpG1, CpG3, CpG4, and smoking correlated with an increased risk of EH (OR = 4.62, adjusted P = 0.011). In addition, CpG2 (adjusted P = 0.013) and CpG3 (adjusted P = 0.039) methylation was significantly lower in healthy males than in healthy females. Likewise, after adjusting for confounding factors, CpG2 methylation (adjusted P = 0.007) still showed significant gender-specific differences among the participants of the study. CpG1 (P = 0.009) site was significantly positively correlated with age, and CpG3 (P = 0.007) and CpG4 (P = 0.006) were both inversely linked to smoking. Our findings suggest that gene-environment interactions are associated with the pathogenesis and progression of EH.
Molecular Medicine Reports | 2017
Shuqi Mao; Tianlun Gu; Fade Zhong; Rui Fan; Fubao Zhu; Peipei Ren; Fengying Yin; Lina Zhang
Molecular Medicine Reports | 2017
Rui Fan; Shu‑Qi Mao; Tian‑Lun Gu; Fa‑De Zhong; Min‑Li Gong; Ling‑Mei Hao; Feng‑Ying Yin; Chang‑Zheng Dong; Li‑Na Zhang