Rui Moriyama
Kyushu University
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Featured researches published by Rui Moriyama.
Topics in Current Chemistry | 2012
Phong Lan Thao Tran; Anne De Cian; Julien Gros; Rui Moriyama; Jean-Louis Mergny
Guanine quadruplexes (G4) are unusual four-stranded nucleic acid structures formed by G-rich DNA/RNA. Beyond their likely biological relevance, the self-assembly, stability, and rigidity of these structures are also interesting for nanotechnology and biotechnology applications. Therefore, efforts are carried out to understand the rules that govern stability and folding of G-quadruplexes. We focus this chapter on tetramolecular conformations which are simple tractable models. We report here the experimental parameters, molecules, and modifications that affect thermal stability and/or association kinetics of these structures. Some chemical modifications which facilitate tetramolecular quadruplex formation and can be useful for nano- or biotechnology are also described.
Biomaterials | 2011
Rui Moriyama; Naohiko Shimada; Arihiro Kano; Atsushi Maruyama
Guanine-rich oligonucleotides, such as TG(4)T and TG(5)T, assemble into a tetramolecular quadruplexes with layers of G-quartets stabilized by coordination to monovalent cations. Association rates of the quadruplexes are extremely slow, likely owing to electrostatic repulsion among the four strands. We have shown that comb-type copolymers with a polycation backbone and abundant hydrophilic graft chains form water-soluble polyelectrolyte complexes with DNA and promote DNA hybridization. Here, we report the effect of cationic comb-type copolymers on the kinetics of tetramolecular quadruplex formation. The copolymer significantly increased the association rate of tetramolecular quadruplexes without altering kinetic effects of metal cations in quadruplex formation. Dissociation rates of the quadruplexes were also accelerated by the copolymer suggesting that the copolymer has chaperone-like activity that reduces the energy barriers associated with dissociation and re-assembly of base pairs. This hypothesis was further supported by the observation that the copolymer activated the strand exchange reaction between the quadruplex and a constituting single-stranded.
Nucleic acids symposium series (2004) | 2009
Rui Moriyama; Naohiko Shimada; Arihiro Kano; Atsushi Maruyama
The intermolecular DNA quadruplex is attractive materials for design of nanomolecular constructs and machines. This folding kinetics is, however, slow, hampering its application to these materials. We have reported that the cationic comb-type copolymer accelerated duplex and triplex formation. In this study, the effect of the copolymer on the intermolecular quadruplex folding was investigated. The copolymer was found to accelerate the association of intermolecular quadruplex by three-orders. Furthermore, the copolymer induced the strand exchange reaction between intermolecular quadruplex and its single-stranded counterparts. We suggested that the copolymer acts as a nucleic acid chaperone for the intermolecular quadruplex.
Journal of Controlled Release | 2007
Ayumi Sato; Sung Won Choi; Miwa Hirai; Asako Yamayoshi; Rui Moriyama; Takeshi Yamano; Motoki Takagi; Arihiro Kano; Akira Shimamoto; Atsushi Maruyama
Langmuir | 2007
Yuichi Sato; Rui Moriyama; Sung Won Choi; and Arihiro Kano; Atsushi Maruyama
Biomaterials | 2011
Rui Moriyama; Naohiko Shimada; Arihiro Kano; Atsushi Maruyama
Current Nanoscience | 2011
Rui Moriyama; Junji Mochida; Asako Yamayoshi; Naohiko Shimada; Arihiro Kano; Atsushi Maruyama
Nucleic acids symposium series (2004) | 2008
Rui Moriyama; Naohiko Shimada; Arihiro Kano; Atsushi Maruyama
Reactive & Functional Polymers | 2007
Rui Moriyama; Sung Won Choi; Naohiko Shimada; Arihiro Kano; Atsushi Maruyama
F1000Research | 2011
Phong Lan Thao Tran; Rui Moriyama; Atsushi Maruyama; Bernard Rayner; Jean-Louis Mergny