Rui Rodrigues

Antimicrobial nanostructured starch based films for packaging

Montmorillonite modified with a quaternary ammonium salt C30B/starch nanocomposite (C30B/ST-NC), silver nanoparticles/starch nanocomposite (Ag-NPs/ST-NC) and both silver nanoparticles/C30B/starch nanocomposites (Ag-NPs/C30B/ST-NC) films were produced. The nanoclay (C30B) was dispersed in a starch solution using an ultrasonic probe. Different concentrations of Ag-NPs (0.3, 0.5, 0.8 and 1.0mM) were synthesized directly in starch and in clay/starch solutions via chemical reduction method. Dispersion of C30B silicate layers and Ag-NPs in ST films characterized by X-ray and scanning electron microscopy showed that the presence of Ag-NPs enhanced clay dispersion. Color and opacity measurements, barrier properties (water vapor and oxygen permeabilities), dynamic mechanical analysis and contact angle were evaluated and related with the incorporation of C30B and Ag-NPs. Films presented antimicrobial activity against Staphylococcus aureus, Escherichia coli and Candida albicans without significant differences between Ag-NPs concentrations. The migration of components from the nanostructured starch films, assessed by food contact tests, was minor and under the legal limits. These results indicated that the starch films incorporated with C30B and Ag-NPs have potential to be used as packaging nanostructured material.

Design of whey protein nanostructures for incorporation and release of nutraceutical compounds in food

ABSTRACT Whey proteins are widely used as nutritional and functional ingredients in formulated foods because they are relatively inexpensive, generally recognized as safe (GRAS) ingredient, and possess important biological, physical, and chemical functionalities. Denaturation and aggregation behavior of these proteins is of particular relevance toward manufacture of novel nanostructures with a number of potential uses. When these processes are properly engineered and controlled, whey proteins may be formed into nanohydrogels, nanofibrils, or nanotubes and be used as carrier of bioactive compounds. This review intends to discuss the latest understandings of nanoscale phenomena of whey protein denaturation and aggregation that may contribute for the design of protein nanostructures. Whey protein aggregation and gelation pathways under different processing and environmental conditions such as microwave heating, high voltage, and moderate electrical fields, high pressure, temperature, pH, and ionic strength were critically assessed. Moreover, several potential applications of nanohydrogels, nanofibrils, and nanotubes for controlled release of nutraceutical compounds (e.g. probiotics, vitamins, antioxidants, and peptides) were also included. Controlling the size of protein networks at nanoscale through application of different processing and environmental conditions can open perspectives for development of nanostructures with new or improved functionalities for incorporation and release of nutraceuticals in food matrices.

Accelerated epipolar geometry computation for 3D reconstruction using projective texturing

The process of <i>3D reconstruction,</i> or depth estimation, is a complex one, and many methods often have several parameters that may require fine tunning to adapt to the scene and improve reconstruction results. Usability of these methods is directly related to their response time. Epipolar geometry, a fundamental tool used in 3D reconstruction, is commonly computed on the CPU. We propose to take advantage of the advances of graphic cards, to accelerate this process. Projective texturing will be used to transfer a significant part of the computational load from the CPU into the GPU. The new approach will be illustrated in the context of a previously published work for 3D point reconstruction from a set of static images. Test results show that gains of up to two orders of magnitude in terms of computation times can be achieved, when comparing current CPUs and GPUs. We conclude that this leads to an increase in usability of 3D reconstruction methods.

Electric field-based technologies for valorization of bioresources

This review provides an overview of recent research on electrotechnologies applied to the valorization of bioresources. Following a comprehensive summary of the current status of the application of well-known electric-based processing technologies, such as pulsed electric fields (PEF) and high voltage electrical discharges (HVED), the application of moderate electric fields (MEF) as an extraction or valorization technology will be considered in detail. MEF, known by its improved energy efficiency and claimed electroporation effects (allowing enhanced extraction yields), may also originate high heating rates - ohmic heating (OH) effect - allowing thermal stabilization of waste stream for other added-value applications. MEF is a simple technology that mostly makes use of green solvents (mainly water) and that can be used on functionalization of compounds of biological origin broadening their application range. The substantial increase of MEF-based plants installed in industries worldwide suggests its straightforward application for waste recovery.

Development of iron-rich whey protein hydrogels following application of ohmic heating – Effects of moderate electric fields

The influence that ohmic heating technology and its associated moderate electric fields (MEF) have upon production of whey protein isolate cold-set gels mediated by iron addition was investigated. Results have shown that combining heating treatments (90°C, 5min) with different MEF intensities let hydrogels with distinctive micro and macro properties - i.e. particle size distribution, physical stability, rheological behavior and microstructure. Resulting hydrogels were characterized (at nano-scale) by an intensity-weighted mean particle diameter of 145nm, a volume mean of 240nm. Optimal conditions for production of stable whey protein gels were attained when ohmic heating treatment at a MEF of 3V∙cm-1 was combined with a cold gelation step using 33mmol∙L-1 of Fe2+. The consistency index of hydrogels correlated negatively to MEF intensity, but a shear thickening behavior was observed when MEF intensity was increased up to 10V∙cm-1. According to transmission electron microscopy, ohmic heating gave rise to a more homogenous and compact fine-stranded whey protein-iron microstructure. Ohmic heating appears to be a promising technique, suitable to tailor properties of whey protein gels and with potential for development of innovative functional foods.

Predictive control of a current-source inverter for solar photovoltaic grid interface

Solar photovoltaic systems are an increasing option for electricity production, since they produce electrical energy from a clean renewable energy resource, and over the years, as a result of the research, their efficiency has been increasing. For the interface between the dc photovoltaic solar array and the ac electrical grid is necessary the use of an inverter (dc-ac converter), which should be optimized to extract the maximum power from the photovoltaic solar array. In this paper is presented a solution based on a current-source inverter (CSI) using continuous control set model predictive control (CCS-MPC). All the power circuits and respective control systems are described in detail along the paper and were tested and validated performing computer simulations. The paper shows the simulation results and are drawn several conclusions.

An intelligent patient monitoring system

Intensive Care Units (ICUs) are a good environment for the application of intelligent systems in the healthcare arena, due to its critical environment that requires diagnose, monitoring and treatment of patients with serious illnesses. An intelligent decision support system - INTCare, was developed and tested in CHP (Centro Hospitalar do Porto), a hospital in Oporto, Portugal. The need to detect the presence or absence of the patient in bed, in order to stop the collection of redundant data concerning about the patient vital status led to the development of an RFID localisation and monitoring system - PaLMS, able to uniquely and unambiguously identify a patient and perceive its presence in bed in an ubiquitous manner, making the process of data collection and alert event more accurate. An intelligent multi-agent system for integration of PaLMS in the hospitals platform for interoperability (AIDA) was also developed, using the characteristics of intelligent agents for the communication process between the RFID equipment, the INTCare module and the Patient Management System (PMS), using the HL7 standard embedded in agent behaviours.

Extending a patient monitoring system with identification and localisation

Intensive Care Units (ICUs) are a good environment for the application of intelligent systems in the healthcare area because it requires diagnosing, monitoring, and treatment of patients with critical illness. An intelligent decision support system, named INTCare, was developed and tested in CHP, a hospital center in Oporto. The need to detect the presence or absence of the patient in room, in order to stop the collection of redundant data concerning about the patient vital status led to the development of an RFID localisation and monitoring system - PaLMS, able to uniquely and unambiguously identify a patient and perceive its presence in room, making the process of data collection and alert event more accurate. The solution was the implementation of an intelligent multi-agent system that connects the Patient Management System module, the INTCare module and the RFID equipment, using the HL7 standard embedded in agents behaviours.

From spatiotemporal curves to reconstructed depth

We present a novel approach for 3D reconstruction based on a set of images taken from a static scene. Our solution is inspired by the spatiotemporal analysis of video sequences. The method is based on a best fitting scheme for spatiotemporal curves that allows us to compute 3D world coordinates of points within the scene. As opposed to a large number of current methods, our technique deals with random camera movements in a transparent way, and even performs better in these cases than with restrained motion such as pure translation. Robustness against occlusion and aliasing is inherent to the method as well.

Electric Field Processing: Novel Perspectives on Allergenicity of Milk Proteins

Milk proteins are being widely used in formulated foods as a result of their excellent technological, functional, and biological properties. However, the most representative proteins from casein and whey fractions are also recognized as major allergens and responsible for the prevalence of cows milk protein allergy in childhood. Electroheating technologies based on thermal processing of food as a result of application of moderate electric fields, also known by ohmic heating (OH) or Joule effect, are establishing a solid foothold in the food industry. Currently, the influence of OH on allergenic aspects of milk proteins is under debate but still undisclosed. The occurrence of electrical effects on the protein structure and its function has already been reported; thus, the impact of OH over allergenicity should not be overlooked. On the basis of these recent findings, it is then relevant to speculate about the impact of this emergent technology on the potential allergenicity of milk proteins.