Ruiyuan Liu
Southern Medical University
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Ruiyuan Liu.
Journal of Materials Chemistry C | 2016
Xiaodong Yang; Xiuli Chen; Xiaodan Lu; Chenggong Yan; Yikai Xu; Xiaodong Hang; Jinqing Qu; Ruiyuan Liu
A novel fluorescence chemosensor 1 with aggregation-induced emission was designed and synthesized through a nucleophilic addition reaction between cyanide and triphenylamine. It exhibited remarkable selectivity and high sensitivity and was able to detect Fe3+, CN− and SO32− in almost pure aqueous solution with low detection limits of 1.44 μM, 9.88 nM and 0.107 μM, respectively. Jobs plot and 1H NMR data showed that the binding stoichiometry of 1 with Fe3+, CN− or SO32− was 1:1. Further observations of 1H NMR titration suggested that a coordination bond was formed between two cyano of 1 and Fe3+ which resulted in fluorescence quenching of 1 after detection of Fe3+, whereas the nucleophilic addition of cyanide or sulfite to the vinyl group was responsible for the fluorescent quenching of CN− or SO32− toward 1. The biological applications of 1 were also evaluated and it was found to exhibit low cytotoxicity and membrane permeability. In addition, 1 could also be made into test strips to detect Fe3+ and CN− and was employed as a sensor for detection Fe3+ in living cells.
Organic and Biomolecular Chemistry | 2013
Xue Yong; Minjian Su; Wen Wang; Yichen Yan; Jinqing Qu; Ruiyuan Liu
A new naked-eye chromogenic chemosensor based on 2-thiohydantoin shows high selectivity for fluoride ions and is used to develop a test paper for detection of fluoride ions in the solid state.
International Journal of Nanomedicine | 2013
Chenggong Yan; Yuankui Wu; Jie Feng; Wufan Chen; Xiang Liu; Peng Hao; Rui-Meng Yang; Juan Zhang; Bingquan Lin; Yikai Xu; Ruiyuan Liu
Purpose Pretargeting of biomarkers with nanoparticles in molecular imaging is promising to improve diagnostic specificity and realize signal amplification, but data regarding its targeting potential in magnetic resonance (MR) imaging are limited. The purpose of this study was to evaluate the tumor angiogenesis targeting efficacy of the anti-αvβ3 antibody guided three-step pretargeting approach with magnetoliposomes. Methods Polyethylene glycol-modified and superparamagnetic iron oxide-encapsulated magnetoliposomes with and without biotin were synthesized and characterized. The cytotoxicity of both probes was evaluated using the methyl thiazdyl tetrazolium assay, and their cellular uptake by mouse macrophage was visualized using Prussian blue staining. Three-step pretargeting MR imaging was performed on MDA-MB-435S breast cancer-bearing mice by intravenous administration of biotinylated anti-αvβ3 monoclonal antibodies (first step), followed by avidin and streptavidin (second step), and by biotinylated magnetoliposomes or magnetoliposomes in the targeted or nontargeted group, respectively (third step). The specificity of αvβ3 targeting was assessed by histologic examinations. Results The developed magnetoliposomes were superparamagnetic and biocompatible as confirmed by cell toxicity assay. The liposomal bilayer and polyethylene glycol modification protected Fe3O4 cores from uptake by macrophage cells. MR imaging by three-step pretargeting resulted in a greater signal enhancement along the tumor periphery, occupying 7.0% of the tumor area, compared with 2.0% enhancement of the nontargeted group (P < 0.05). Histologic analysis demonstrated the targeted magnetoliposomes colocalized with neovasculature, which was responsible for the MR signal decrease. Conclusion These results indicate that our strategy for MR imaging of αvβ3-integrin is an effective means for sensitive detection of tumor angiogenesis, and may provide a targetable nanodelivery system for anticancer drugs.
New Journal of Chemistry | 2014
Ruiyuan Liu; Ya Gao; Qianbing Zhang; Xiaodong Yang; Xinwei Lu; Zhiyong Ke; Wenqiang Zhou; Jinqing Qu
A novel ratiometric fluorescent probe based on hydroxylnaphthalene 2-cyanoacrylate was developed to act as a luminescent probe for biological fluoride ion detection. The probe is responsive and highly selective for fluoride ions over other common anions; it also exhibits a very low detection limit of 8.54 μM. The deprotonation of the phenol moieties leads to a fluorescent change in the probe. The biological applications of the probe were also evaluated and it was found to exhibit a low cytotoxicity, a good water solubility and membrane permeability. The probe was, therefore, employed as a sensing probe for the detection of fluoride ions in living cells.
New Journal of Chemistry | 2013
Xue Yong; Mingjian Su; Wen Wan; Wenwei You; Xinwei Lu; Jinqing Qu; Ruiyuan Liu
A novel anion receptor based on 2-thiohydantoin, containing different hydrogen bonding donors such as OH and NH groups, was synthesized. This receptor showed a colorimetric response to fluoride ions. 1H NMR titration confirmed the deprontonation of the phenol OH group and hydrogen bonding interaction between NH group and F−.
RSC Advances | 2014
Xiaodong Yang; Weifeng Zeng; Lei Wang; Xinwei Lu; Yichen Yan; Jinqing Qu; Ruiyuan Liu
A highly sensitive fluorescent probe (1) based on styrylcyanine dye for Cu2+ and Pb2+ has been developed. Probe 1 exhibited fluorescent turn-off sensing ability to Cu2+. On the other hand, 1 displayed ratiometric fluorescent response towards Pb2+ with a distinct fluorescent color change from blue to orange. The 1H NMR titrations revealed that the fluorescent response of 1 to Cu2+ and Pb2+ is triggered by the interaction of the pyridine unit and the metal ion. Detection limits of 1 to Cu2+ or Pb2+ were calculated as 1.24 and 3.41 × 10−6 M, respectively, by standard deviation and linear fitting. Furthermore, 1 can also be used as a sensor for detection of Cu2+ and Pb2+ in a test strip.
Polymer Chemistry | 2013
Xue Yong; Wen Wan; Mingjian Su; Wenwei You; Xinwei Lu; Yichen Yan; Jinqing Qu; Ruiyuan Liu; Toshio Masuda
A novel poly(phenyleneethynylene) containing thiourea groups [poly(1)] was synthesized. The anion sensing ability of poly(1) was assessed using tetra-n-butylammonium (TBA) salts of a series of anions in DMF. The addition of F− changed the color of the polymer solution in DMF from yellow to orange and quenched the fluorescence, whereas the visible color and fluorescence of the solution only varied slightly upon the addition of Cl−, HSO4−, Br−, CH3COO−, C6H5COO−, and NO3−; this indicates that poly(1) exhibits an F− sensing ability. 1H NMR titrations of poly(1) revealed that the colorimetric and fluorescent response of poly(1) is triggered by thiourea/F− hydrogen bonding. Poly(1) also exhibited a turn-off fluorescence Ag+ sensing ability. The addition of Ag+ quenched the fluorescence of the polymer, while the addition of Pb2+, Zn2+, Cu2+, Mn2+, K+, Na+, and Co2+ resulted in only slight changes to the fluorescence. The sensor containing poly(1) and Ag+ shows a turn-on fluorescence adenosine diphosphate (ADP) sensing ability.
Chinese Journal of Polymer Science | 2016
Xiuli Chen; Weifeng Zeng; Xiaodong Yang; Xinwei Lu; Jinqing Qu; Ruiyuan Liu
A novel conjugated polymer, poly(1), containing thiourea moieties in main chain is synthesized via Suzuki coupling reaction. The addition of cuprous ion quenches the fluorescence of poly(1), whereas the fluorescence changes slightly upon addition of other metal ions, exhibiting the fluorescent almost turn-off sensing ability towards Cu+. When hydrogen peroxide was added to the solution containing poly(1) and Cu+, Cu+ was oxidized into Cu2+, resulting in the fluorescence recovery. The H2O2 released from glucose oxidation by glucose oxidase (GOD) also recovered the fluorescence of poly(1)/Cu+ solution. The results indicated that the poly(1)/Cu+ solution could serve as a sensing platform for hydrogen peroxide and glucose.
Macromolecular Bioscience | 2015
Lixia Huang; Chenggong Yan; Danting Cui; Yichen Yan; Xiang Liu; Xinwei Lu; Xiangliang Tan; Xiaodan Lu; Jun Xu; Yikai Xu; Ruiyuan Liu
Nitroxides have great potential as magnetic resonance imaging (MRI) contrast agents for tumor detection. Polyacetylenes(PAs) containing 2,2,6,6-tetramethyl-piperidine oxyl (TEMPO) and poly(ethylene glycol) were synthesized via metathesis polymerization of the corresponding substituted acetylenes to be used for targeted bimodal MRI /optical imaging of tumors. The poly(ethylene glycol) in the polyacetylenes enables covalent conjugation of carboxyl fluorescein and folic acid (FA) with hydroxyl groups to develop targeted multifunctional organic radical contrast agents (ORCAs). In vitro studies confirm the excellent binding specificity and subsequent enhanced cellular internalization of the targeted ORCAs (PA-TEMPO-FI-FA) without cytotoxicity. In vivo T1-weighted MRI demonstrates the active tumor targeting ability of PA-TEMPO-FI-FA to generate specific contrast enhancement in mice bearing HeLa tumors. Moreover, longitudinal optical imaging displays high tumor accumulation after 1 h post-injection of PA-TEMPO-FI-FA. These results indicate that multifunctional ORCAs may provide a tumor-targeted delivery platform for further molecular imaging guided cancer therapy.
Chinese Journal of Polymer Science | 2013
Mingjian Su; Wen Wan; Xue Yong; Xinwei Lu; Ruiyuan Liu; Jinqing Qu
Novel acetylenes carrying urea groups, 1-(4-ethynylphenyl)-3-(4-nitrophenyl) urea (1), 1-(4-propargyl)-3-(4-nitrophenyl) urea (2), were synthesized and polymerized with rhodium catalyst. Polymers [poly(1) and poly(2)] with moderate molecular weights were obtained in good yields. The anion sensing ability of poly(1) and poly(2) was estimated using the tetra-n-butylammonium (TBA) salts of a series of anions in DMF. Upon the addition of F−, the color of the DMF solution of poly(1) and poly(2) immediately turned to a different color, while the color of solution changed slightly upon addition of Cl−, HSO4−, Br−, and NO3−, indicating the F− sensing ability of poly(1) and poly(2). The 1H-NMR titrations of poly(1) revealed that the colorimetric response of poly(1) was triggered by the urea/F− interaction through the hydrogen bonding and/or deprotonation process. The absorption spectra titration and Hill plot analysis were carried out to measure the F− binding ability, and the Hill coefficient in the poly(1)/F− complexation was found to be 5.8. This result clearly indicated that this binding mode between poly(1) and F− was based on a positive homotropic allosterism.