Rupi Prasad

Esterase, Malate, and Lactate Dehydrogenases Activity in Murine Neuroblastoma

Mouse neuroblastoma tumors have only the fifth isozyme band (A4) of lactate dehydrogenase, whereas this band is missing in the brain which contained four other bands of lactate dehydrogenase. The α-esterase isozyme patterns of tumors, kidney, and brain are similar except that there is an additional slowest-moving form of esterase in all tumor tissues. The malate dehydrogenase pattern is not altered in any of the tissues.

Expression of Lactate and Malate Dehydrogenases in Tumors Induced by SV40 and 7,12-Dimethylbenz(a)anthracene

Isozyme patterns of lactate and malate dehydrogenases were studied in tumors induced by SV40 and 7,12-dimethylbenz(a)anthracene and in established cultures of cells from these tumors. The expression of B polypeptide subunits of lactate dehydrogenase is suppressed similarly by both agents. This may be due to inactivation of the gene at the locus determining the B polypeptide subunit. Malate dehydrogenase isozyme patterns are not changed significantly by the virus or the carcinogen.

Lactate and Malate Dehydrogenase and α-Esterases in Oligospermia

Lactate dehydrogenase (LDH), malate dehydrogenase, and α-esterase were studied electrophoretically in a total of 99 semen samples obtained from normal, vasectomized, oligospermic, and infertile males. The enzymatic patterns were compared with total sperm count and percentage sperm motility. Lactate dehydrogenase X was absent in semen samples from oligospermic as well as vasectomized males. An extra LDH band (between the second and third LDH bands) was detectable in samples which had a low sperm count but higher motility (80 to 90%). This extra band was absent in samples with higher sperm count regardless of the level of percentage motility, suggesting that the extra band possibly may be related to motility factors of low sperm count but not normal sperm count. The fastest moving α-esterase bands were absent in samples from oligospermic or vasectomized males. These bands were also absent in samples from infertile patients having a very low sperm count. It is not clear whether the fast-moving esterase bands are related to sperm count or possible differences in hormonal levels of individuals with low or zero sperm count samples. The patterns of malate dehydrogenase did not differ in individuals with zero, low, or normal sperm count.

Relationship between mitogen response and aryl hydrocarbon hydroxylase in cultured human lymphocytes

Abstract This study was conducted to determine if any relationship existed between Aryl hydrocarbon hydroxylase (AHH) inducibility and mitogen response of cultured lymphocytes from normal healty individuals. The experiments were done repeatedly on the peripheral blood lymphocytes of the same individuals at 4 months intervals during the course of a year. AHH activity was measured by spectrophotofluorometric assay procedures. Mitogen response was measured by counting the incorporation of 3H-Thymidine in cellular DNA using a liquid scintillation counter. Our study indicated large variations in mitogen response of the lymphocytes throughout the study period within the same individuals as well as among the individuals. Variations in AHH inducibility within and among the individuals were also found. The variations were without any pattern. No relationship was found between AHH inducibility and the mitogen response index, suggesting the AHH inducibility test in the mitogen stimulated cultured lymphocytes is not affected by the immune responsiveness of the cells from individual.

Electrophoretic patterns of glucose metabolizing enzymes and acid phosphatase in mouse and human neuroblastoma cells.

Abstract The electrophoretic patterns of glucose metabolizing enzymes and acid phosphatase in mouse and human neuroblastoma cells were investigated. Mouse neuroblastoma cells had one band of lactate dehydrogenase (LDH) and two bands of acid phosphatase, whereas human neuroblastoma cells had five bands of LDH and one band of acid phosphatase. Glucose-6-phosphate dehydrogenase (G-6-PD) and 6-phosphogluconate dehydrogenase (6-PGD) were expressed as a single band in both mouse and human neuroblastoma cells. The electrophoretic pattern of LDH was similar in mouse neuroblastoma cells grown in culture or in vivo. The electrophoretic band of G-6-PD in mouse neuroblastoma cells grown in vivo appeared to be less dense than that observed in cells grown in culture; however, the reverse was true for 6-PGD. Among all enzymes examined, only the electrophoretic pattern of G-6-PD in cAMP-induced “differentiated” mouse neuroblastoma was different in comparison to control cells.

Lymphocyte Replication in Lung Cancer Patients Undergoing Radiotherapy

In vivo and in vitro survival and response of lymphocytes were studied in 12 lung cancer patients before, during and following radiotherapy, and comparisons were made to data from 20 healthy control subjects. Lymphocyte counts of lung cancer patients prior to radiotherapy did not differ significantly from those of control subjects. Following radiotherapy, lymphocyte counts were significantly reduced. The in vitro survival of lymphocytes from lung cancer patients prior to radiotherapy was lower than that of normal controls. Radiation treatment had no effect on lymphocyte survival in vitro. PHA-stimulated lymphoblast formation in lung cancer patients prior to radiotherapy did not differ from that of normal controls, but decreased significantly during radiotherapy.

Enzymatic differentiation in human amniotic cells

Enzymatic study in noncultured amniotic fluid cells from 87 women at different stages of pregnancy was performed by electrophoresis. Of these patients, 33 had normal pregnancies and the remaining 54 had various complications of pregnancy. The electrophoretic patterns of lactate dehydrogenase, alpha-esterase, and acid phophatase were altered in samples obtained either at different gestation periods or from normal and complicated pregnancies. Malate dehydrogenase and protein bands did not show any alteration. Glucose-6-phosphate dehydrogenase and alkaline phophatase were not detectable. 6-Phosphogluconate dehydrogenase was detectable in samples of complicated pregnancies. The significance of the altered patterns of enzymes is discussed.