Ruth E. McDiarmid

Content of zygacine in Zygadenus venenosus at different stages of growth

Abstract A TLC-scanning procedure was developed for the quantitative determination of zygacine in death camas. The alkaloid levels increased during the pod stage of development when the veratryl and angelyl esters of zygadenine were also detected at high concentrations.

AUTOLYSIS OF THLASPI ARVENSE IN BOVINE RUMEN FLUID

Abstract The formation of allylthiocyanate (ATC) in bovine rumen fluid requires the presence of the hydrolytic plant enzyme from Thlaspi arvense. Sinigrin, alone, was not hydrolysed in vitro but allylisothiocyanate was formed in the presence of erogenous myrosinase. The ATC potential of T. arvense is described.

3-nitropropyl glycosides ofAstragalus miser var.serotinus

Abstract On the basis of spectroscopic and chemical evidence, 3-nitropropyl β- d -cellobioside was identified among the toxic glycosides produced by Astragalus miser var. serotinus and its structure confirmed by synthesis, as was that of the β- d -gentiobioside previously isolated from this plant.

TLC Luminescence of gramine and related indole alkaloids in phalaris arundinacea

Abstract Luminescence characteristics of gramine, dimethyltryptamine and related indoles on Si gel TLC are described, chromatographic conditions are devised which enhance the luminescence, and a fluorometric TLC scanning procedure is developed for the quantitative determination of gramine in Phalaris arundinacea . 5-Methoxy-and 5-hydroxyindoles are readily distinguished from the indoles lacking a polar substituent at position 5.