Ryan M. Bouldin

What Are Students Doing during Lecture? Evidence from New Technologies to Capture Student Activity.

Engaging students in class is paramount if they are to gain a deep understanding of class content. Student engagement is manifested by attention to the various components of instruction. However, there is little research at the tertiary level focusing on what aspects of instruction are related to changes in student attention during class. To address this gap, we collected multiple streams of data that provide a measure of student attention during instruction. We had students in an organic chemistry course who wear hats with a camera mounted on the brim, to provide a record of student gaze (i.e. looking at the board, notes, and friends). We also had students who use electronic pens that allowed us to record what information students transferred into their notes (pencasts). Based on our initial results, we believe the data provided by the point-of-view cameras and electronic pens hold great promise for using these technologies as viable research tools in educational settings to address various research questions.

Oxidoreductase Catalyzed Polymerization of 3-Methylpyrrole

A one-pot, environmentally friendly enzymatic method is described for the synthesis a polypyrrole derivative, poly(3-methylpyrrole) (poly(3-MP)) in an aqueous solvent system. The enzyme, soybean peroxidase (SBP), was shown to successfully polymerize the beta functionalized pyrrole monomer. The resulting polymer was semiconducting, thermally stable, and exhibited bipolaron absorptions when synthesized in the presence of poly(sodium 4-styrenesulfonate) (PSS). Polymers synthesized in the presence of 10-camphor sulfonic acid (CSA) were found to exhibit slightly higher conductivity values, but were less thermally stable compared to poly(3-MP) polymerized in the presence of PSS. This work should stimulate further interest in expanding the use of enzymes as catalysts for the polymerization of other pyrrole derivatives, as well as other classes of conjugated polymers.

Biocatalytic Synthesis of Fluorescent Conjugated Indole Oligomers

Fluorescent conjugated materials exhibiting reasonable biocompatibility that are capable of interacting with biological molecules are of interest for bio-sensing and imaging applications. Traditional approaches do not allow for the synthesis of conjugated materials in the presence of biologically relevant substrates. Further conjugated polymers synthesized using conventional methods are doped and not fluorescent. Here we explore the possibility of synthesizing fluorescent oligomers of indole using enzymes as catalyst under mild conditions. The peroxidase catalyzed coupling reaction presented here creates a photoluminescent material that allows for direct utilization (without purification and separation of the dopant) in biosensing applications. The polymerization reaction proceeds smoothly in just deionized water and ethanol. Monitoring of the absorption and fluorescence spectra over one hour shows that the concentration of both absorbing and emitting species grows steadily over time. The presence of anionic buffers and templates is shown to effectively retard the development of light emitting species and instead leads to the formation of an electrically doped conjugated polymer. Structural characterization through FTIR and ¹H-NMR analysis suggests that the oligomer is coupled through the 2 and 3 positions on the indole ring.