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Ryo Okachi

University of Tokyo

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Publication

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Agricultural and biological chemistry | 1975

Purification and Properties of Penicillin Acylase from Kluyvera citrophila

Mikio Shimizu; Ryo Okachi; Kazuo Kimura; Takashi Nara

Penicillin acylase (EC 3.5.1.11) of Kluyvera citrophila KY7844 was purified approximately 120-fold by DEAE-cellulose chromatography, hydroxyapatite chromatography and isoelectro-focusing fractionation. The purified enzyme, with an approximate molecular weight of 63,000, appeared to be homogeneous in disc electrophoretic analysis, and showed isoelectric point (Ip) 8.12 and 13.0 units/mg of specific activity for cephalexin hydrolysis. The Michaelis constant (Km) for cephalexin and for 7-[1-(1H)-tetrazolylacetamido]-desacetoxycephalosporanic acid ((1H) T-7ADCA) was 1.4 mM and 3.6 mM, respectively. This enzyme was capable of producing (1H) T-7ADCA in 80% yield from 1-(1H)-tetrazolylacetate methylester and 7-aminodesacetoxycephalosporanic acid.

The Journal of Antibiotics | 1977

FORTIMICINS A AND B, NEW AMINOGLYCOSIDE ANTIBIOTICS

Takashi Nara; Mitsuyoshi Yamamoto; Isao Kawamoto; Kenichiro Takayama; Ryo Okachi; Seigo Takasawa; Tomoyasu Sato; Seiji Sato

The Journal of Antibiotics | 1977

Fortimicins A and B, new aminoglycoside antibiotics. II. Isolation, physico-chemical and chromatographic properties.

Ryo Okachi; Seigo Takasawa; Tomoyasu Sato; Seiji Sato; Mitsuyoshi Yamamoto; Isao Kawamoto; Takashi Nara

The Journal of Antibiotics | 1977

Fortimicins A and B, new aminoglycoside antibiotics. I. Producing organism, fermentation and biological properties of fortimicins.

Takashi Nara; Mitsuyoshi Yamamoto; Isao Kawamoto; Kenichiro Takayama; Ryo Okachi; Seigo Takasawa; Tomoyasu Sato; Seiji Sato

The Journal of Antibiotics | 1974

A new antibiotic XK-62-2 (Sagamicin). I. Isolation, physicochemical and antibacterial properties.

Ryo Okachi; Isao Kawamoto; Seigo Takasawa; Mitsuyoshi Yamamoto; Seiji Sato; Tomoyasu Sato; Takashi Nara

The Journal of Antibiotics | 1986

K-26, A NOVEL INHIBITOR OF ANGIOTENSIN I CONVERTING ENZYME PRODUCED BY AN ACTINOMYCETE K-26

Masayuki Yamato; Toshiro Koguchi; Ryo Okachi; Koji Yamada; Kiyoshi Nakayama; Hiroshi Kase; Akira Karasawa; Katsuichi Shuto

Archive | 1974

Fortimicin B and process for production thereof

Takashi Nara; Seigo Takasawa; Ryo Okachi; Isao Kawamoto; Mitsuyoshi Yamamoto

Agricultural and biological chemistry | 1975

Search for Microorganisms Producing Cephalosporin Acylase and Enzymatic Synthesis of Cephalosporins

Mikio Shimizu; Taketoshi Masuike; Hironori Fujita; Kazuo Kimura; Ryo Okachi; Takashi Nara

Agricultural and biological chemistry | 1975

Enzymatic synthesis of cphalosporins. III. Purification and properties of penicillin acylase from Kluyvera citrophila.

Mikio Shimizu; Ryo Okachi; Kazuo Kimura; Takashi Nara

The Journal of Antibiotics | 1987

Aminothiazolylglycyl derivatives of carbacephem antibiotics. II: Synthesis and antibacterial activity of novel aminothiazolyl cephem compounds with hydroxypyridone moiety

Kenichi Mochida; Yasuyuki Ono; Motoo Yamasaki; Chihiro Shiraki; Tadashi Hirata; Kiyoshi Sato; Ryo Okachi

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Takashi Nara

University of Tokyo

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Isao Kawamoto

University of Tokyo

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Seigo Takasawa

Kyoto University

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Mitsuyoshi Yamamoto

University of Tokyo

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Seiji Sato

University of Tokyo

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Tomoyasu Sato

University of Tokyo

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Kiyoshi Sato

Juntendo University

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Kenichi Mochida

Biotechnology Institute

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Tadashi Hirata

University of Tokyo

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Kunikatsu Shirahata

Ohio State University

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