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Featured researches published by Ryo Soda.


Respirology | 2008

Inflammatory Markers in Exhaled Breath Condensate from patients with Asthma.

Tomoe Ueno; Mikio Kataoka; Atsushi Hirano; Kouji Iio; Yasushi Tanimoto; Arihiko Kanehiro; Chiharu Okada; Ryo Soda; Kiyoshi Takahashi; Mitsune Tanimoto

Background and objective:  Evaluation of airway inflammation is important for the diagnosis and treatment of asthma. Exhaled breath condensate (EBC) is a minimally invasive method for assessing inflammation and may be useful for monitoring airway inflammation in asthma. The aims of this study were to establish an EBC collection method, to assess biomarkers reflecting asthmatic airway inflammation, and to determine the relationship of these biomarkers with asthma severity and lung function.


Journal of Immunological Methods | 1993

New flow cytometric method for surface phenotyping basophils from peripheral blood

Kiyoshi Takahashi; Minoru Takata; Toshimitsu Suwaki; Noriko Kawata; Yasushi Tanimoto; Ryo Soda; Ikuro Kimura

To clarify the role of basophils in the pathogenesis of allergic disease, we developed a new method for performing surface phenotyping of these cells in centrifugation-enriched mononuclear cell fraction. This method identified basophils on the basic of a negative reactivity with mixed FITC-conjugated monoclonal anti-bodies (mAbs) (anti-CD2, -CD14, -CD16, and -CD19) with analysis performed by flow cytometry. The validity of this approach was confirmed by sorting experiments. Various PE-conjugated mAbs were also used to examine binding to FITC-negative basophils. Basophils from asthmatic patients (n = 14) as well as from normal subjects (n = 6) were shown to express CDw32 (Fc gamma RII), CD25 (IL-2R), but not CD64 (Fc gamma RI). We also detected binding of IgG1 and IgG4 to basophils. This method of phenotyping was very rapid and simple. It thus appears to be useful in the study of allergic disease, as well as of the biology of the basophil.


Leukemia & Lymphoma | 2001

Epstein-Barr Virus Associated Diffuse Large B-Cell Lymphoma Complicated by Autoimmune Hemolytic Anemia and Pure Red Cell Aplasia

Hideki Katayama; Makoto Takeuchi; Tadashi Yoshino; Mttsuru Munemasa; Atsuhiko Tada; Ryo Soda; Kiyoshi Takahashi

A 53-year old man with systemic lymphadenopathy and hepatosplenomegaly was diagnosed with diffuse large B cell-lymphoma after inguinal lymph node biopsy. Anemia was noted, direct and indirect Coombs tests were positive, and the haptoglobin level was low. However, the bone marrow aspirate revealed erythroid aplasia. Co-existing autoimmune haemolytic anemia (AIHA) and pure red cell aplasia (PRCA) were diagnosed. In situ hybridization with Epstein-Barr virus (EBV) encoded small RNA (EBER) showed positive findings in lymphoma cells. Southern blot hybridization revealed immunoglobulin heavy chain gene rearrangement and a clonal EBV terminal repeat, indicating monoclonal proliferation of EBV in infected B cells. The patient was treated with CHOP, resulting in a complete remission (CR). AIHA and PRCA subsided after 3 courses of chemotherapy. In conclusion, this case demonstrates not only the association of B-cell lymphoma with autoimmune disorders but also the involvement of EBV in these conditions.


Journal of Asthma | 2005

Successful Treatment of a Patient with Severe Churg-Strauss Syndrome by a Combination of Pulse Corticosteroids, Pulse Cyclophosphamide, and High-Dose Intravenous Immunoglobulin

Nagio Takigawa; Noriko Kawata; Takuo Shibayama; Atsuhiko Tada; Goro Kimura; Mitsuru Munemasa; Ryo Soda; Kiyoshi Takahashi

A 24-year-old woman with a 4-year history of bronchial asthma suffered from bloody sputum, numbness of the extremities, elevated eosinophil count, and hypoxemia. A diagnosis of alveolar hemorrhage was made by bronchoalveolar lavage. Echocardiogram revealed severe hypokinesis of the left ventricular wall. Her respiratory condition deteriorated despite administration of pulse corticosteroids. A second pulse corticosteroids and pulse cyclophosphamide followed by high-dose intravenous immunoglobulin brought about a dramatic improvement of alveolar hemorrhage, cardiac impairment, and peripheral neuropathy. Levels of antimyeloperoxidase-antineutrophil cytoplasmic antibodies, soluble thrombomodulin, soluble interleukin-2 receptor, eosinophil cationic protein were elevated and returned to the normal range in remission. The combination of pulse corticosteroids, pulse cyclophosphamide, and high-dose intravenous immunoglobulin seemed effective for the acute phase of severe Churg-Strauss syndrome.


International Archives of Allergy and Immunology | 2001

A Study of Clinical Features of Cough Variant Asthma

Chiharu Okada; Masahide Horiba; Hiroshi Matsumoto; Rikako Torigoe; Hidetsugu Mizuuchi; Masaharu Murao; Ryo Soda; Kiyoshi Takahashi; Goro Kimura; Yasushi Tanimoto

Patients with cough variant asthma (CVA) and classic asthma are frequently among subjects who present at clinics complaining of a chronic persistent cough. To reveal the features of CVA, we examined the differences in the clinical appearance between CVA and classic asthma. Ten CVA subjects and 11 classic asthmatics were enrolled in the study; they were recruited among patients who presented at the National Minamiokayama Hospital complaining of a chronic cough. The number of eosinophils in peripheral blood was 256 ± 45.8/µl in CVA and 400 ± 123/µl in classic asthma. Eosinophils represented 67% of the cells of sputum in CVA and 82% in classic asthma. Bronchial responsiveness to methacholine was Dmin 1.37 ± 0.56 U in CVA and 0.71 ± 0.46 U in classic asthma. There was no significant difference in these three parameters. There was only a significant difference in v̇25 between CVA and classic asthma, 80.0 ± 6.9 and 52.2 ± 10.0%, respectively. Eosinophil inflammation was almost the same in both CVA and classic asthma.


International Archives of Allergy and Immunology | 1996

Signal transduction by IgG receptors induces calcium mobilization, but not histamine release, in the human basophilic cell line KU812F.

Noriko Kawata; Minoru Takata; Toshimitsu Suwaki; Yasushi Tanimoto; Ryo Soda; Kiyoshi Takahashi; Ikuro Kimura

Human blood basophils selectively express Fc gamma RII (CDw32) among IgG receptor subtypes, but its functional role in allergic reactions remains unknown. Using the human basophilic leukemia cell line KU812F as a model system, we investigated cellular signaling events mediated through IgG receptor stimulation. KU812F cells express Fc gamma RII on their surface. mRNAs for both Fc gamma RIIA and IIB subtypes were detected by reverse transcriptase-PCR analysis. In this cell line, Fc gamma RII stimulation induced mobilization of free intracellular calcium and actin polymerization. Yet, no significant histamine release was observed, nor did blood basophils stimulated by anti-Fc gamma RII monoclonal antibody IV.3 and by a secondary antibody release histamine. These data indicate that Fc gamma RII stimulation induces cellular signaling events such as calcium mobilization in human basophils. However, these events do not lead to histamine release.


Annals of Nuclear Medicine | 1998

Influence of age and gender on iodine-123 MIBG kinetics in normal lung

Noriko Kanzaki; Ryo Soda; Kiyoshi Takahashi; Keiko Sato; Toshiyuki Hayabara; Masayoshi Kibata; Yoshihiro Takeda; Yoshio Hiraki

Iodine-123 MIBG is a biochemical marker that can be used to monitor pulmonary norepinephrine (NE) metabolism. The purpose of this study was to characterize pulmonary I-123 MIBG kinetics in relation to age and gender.Materials and MethodsSeventeen healthy volunteers and 14 patients with no cardiac or pulmonary disorders were included in this study (age range: 24 to 88 years, mean age 50.2 ± 17.6 years; 16 males, 15 females). Planar images were obtained 15 min (early) and 3 h (delayed) after injection of I-123 MIBG (111 MBq). Pulmonary uptake of I-123 MIBG was quantified based on the lung-to-mediastinum ratio (LMR) on early and delayed images. The lung clearance rate (LCR) was calculated from both the early and delayed images.ResultsBoth early and delayed LMR values increased slightly, although they showed no significant correlations with age. There was a significant inverse correlation between LCR and age (r = −0.57, p < 0.001). Neither LCR nor LMR differed significantly between male and female patients, but the mean age of the men was lower than that of the women.ConclusionsPulmonary I-123 MIBG kinetics may reflect age-dependent changes in NE metabolism. The effects of age should be taken into account when assessing pulmonary NE metabolism with I-123 MIBG.


International Journal of Hematology | 2003

Pharmacokinetics of a standard dose of cytarabine in a patient with acute promyelocytic leukemia undergoing continuous ambulatory peritoneal dialysis

Makoto Takeuchi; Takahiro Yamauchi; Isao Yoshida; Ryo Soda; Takanori Ueda; Kiyoshi Takahashi

Dose setting for drugs to treat acute leukemia in patients undergoing dialysis therapy is a critical problem that is complicated by incomplete knowledge of the underlying pharmacokinetics of antileukemic agents in this setting, especially in continuous ambulatory peritoneal dialysis (CAPD) [1]. Because impaired renal function, permeability of the peritoneal membrane, the protein-binding ratio of the drug, and the CAPD schedule, along with other factors, are likely to influence plasma drug concentrations, the pharmacokinetics of individual antileukemic drugs must be evaluated. We report pharmacokinetic observations for a standard dose of cytarabine, the key drug for treating myeloid leukemia, in a patient with acute promyelocytic leukemia (APL) who was undergoing CAPD.


Clinical & Experimental Allergy | 1983

New in vitro method for detecting asthma allergen. Counting reactive basophils after addition of allergen

Ikuro Kimura; Yoshiro Tanizaki; Yoshinori Goda; Yoshihide Sasaki; Katsumi Akagi; Makoto Kobayashi; Ryo Soda

Changes in the morphology and number of basophils were examined after the addition in vitro of house dust extract in cells from seventy‐seven patients with bronchial asthma. Morphological changes (reactive basophils) showed a close relation to the end‐point titrations of antigen in skin tests, RAST score and bronchial provocation tests, but changes in number of basophils did not. With a RAST score of 2 or greater the change in reactive basophils varied from 40 to 80% (mean 58‐1%), which was much greater than seen in persons with no RAST, score 0. Patients with positive bronchial inhalation tests, showed 40 to 80% (mean 57.8%) reactive basophils, whereas persons with a negative bronchial challenge test showed a range of reactive basophils from 0 to 50% (mean 28.9%). The difference in percentage of reactive basophils between the positive and negative groups of persons tested by bronchial inhalation was statistically significant.


Clinical & Experimental Allergy | 1985

Basophil immunoglobulin receptors in asthmatics under immunoscanning electron microscopy

Ikuro Kimura; Kiyoshi Takahashi; Ryo Soda; Takumi Kishimoto; Takashi Matsuoka

Immunolatex particles were used as markers for IgE receptors on basophils using electron‐microscope scanning. These particles appeared to bind specifically to basophils. Basophils from atopic asthma patients showed greater binding to latex particles than those from intractable asthma patients or healthy subjects. Cap and patch formations on IgE receptors were frequently found in basophils of atopic asthmatics, and cap formation was observed only on basophils that were pear‐shaped. The redistribution of IgE receptors, such as that by cap formation, may be a significant triggering mechanism for basophil activation.

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