Ryohei Nagata

A glucose sensor fabricated by the screen printing technique

A novel glucose sensor employing ferrocene-modified glucose oxidase is fabricated using the screen printing technique. Glucose oxidase is covalently bound to the electron mediator ferrocenecarboxylic acid in order to obtain higher enzyme activity. The ferrocene-glucose oxidase shows an increased catalytic current because the ferrocene acts as an electron transfer relay between the active centre of the enzyme and the gold electrode. Glucose sensors employing enzymes modified with ferrocene in various ways are successfully fabricated using the screen printing technique. The ink component containing the ferrocene-glucose oxidase is specially developed to be applicable to the printing machine. The printed glucose sensor chip offers a stable calibration profile and stable electrochemical properties.

Mass transport behavior of electrochemical species through plasma-polymerized thin film on platinum electrode

Abstract We propose a novel method for generating thin film coating for use on a platinum (Pt) electrode. This is accomplished by a plasma-polymerized film (PPF), which is deposited directly onto the substrate under dry condition. The resulting films are extremely thin (

Amperometric glucose biosensor manufactured by a printing technique

Abstract A glucose sensor was fabricated using the screen printing technique with a special ink component containing ferrocene-bound glucose oxidase (fc-GOD). Glucose oxidase was modified with ferrocene derivatives as the electron-transfer mediator in order to obtain higher GOD activity. The ferrocene-modified GOD showed an increase in catalytic current. A special ink component compatible with the printing process was prepared with fc-GOD, soluble and insoluble polymer resins and organic solvent. Using the conventional screen printing technique, the ink component was printed on a thin gold film electrode on a plastic film. The output of the printed glucose sensor was observed to be stable for several hours and proportional to the glucose concentration from 0.5 to 2.0 mg ml−1.