Ryoichi Mitsuzono

Effect of repeated exercise on urinary 8-hydroxy-deoxyguanosine excretion in humans.

The purpose of this study was to investigate the effect of repeated exercise on oxidative damage to DNA in 10 well trained long distance runners who participated in an 8-day training camp. The average running distance during the camp was 30 +/- 3 km/day. The amount of urinary 8-hydroxy-deoxyguanosine (8-OHdG) excretion was used to estimate the oxidative DNA damage. Urine samples were collected for both a 3-day control period as well as throughout the camp. Blood samples were drawn after overnight fasting both before and after the camp. Urinary 8-OHdG excretion was significantly increased during the camp compared to the control period (265.7 +/- 75.5 vs. 335.6 +/- 107.4 pmol/kg/day, P < 0.05). The content of 8-OHdG in the lymphocyte DNA on the day after finishing the camp did not differ from that before the camp. Plasma TBARS, LDH, CK, CK-MB, and myoglobin significantly rose after the camp (P < 0.05). The plasma beta-carotene levels tended to rise after the camp, while the plasma alpha-tocopherol levels increased significantly after the camp (P < 0.05). These results indicate that repeated exercise augments oxidative stress and the DNA is also injured by exercise-induced reactive oxygen species. However, the oxidative damage to DNA is not accumulated by consecutive exercise, although it is sustained as long as the exercise is repeated.

Longitudinal associations between anaerobic threshold and distance running performance

SummaryLongitudinal alterations in anaerobic threshold (AT) and distance running performance were assessed three times within a 4-month period of intensive training, using 20 male, trained middle-distance runners (19–23 yr). A major effect of the high intensity regular intensive training together with 60- to 90-min AT level running training (2d ·wk−1) was a significant increase in the amount of O2 uptake corresponding to AT (

Branched-Chain Amino Acid Supplementation Increases the Lactate Threshold during an Incremental Exercise Test in Trained Individuals

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Comparison of Methods for Estimating Percent Body Fat in Distance-Runners

AT; ml O2 · min−1 · kg−1) and in maximal oxygen uptake (

BODY COMPOSITION AND SOMATOTYPE IN ELITE FEMALE DISTANCE RUNNERS

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O-14 Effects of branched-chain amino acid supplementation on lactate threshold by cycle ergometer exercise(The Proceedings of the 12th Annual Meetings of Japan Society of Exercise and Sports Physiology)

; ml O2 · min−1 · kg−1). Both