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Ryouji Karinaga

University of Kitakyushu

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Publication

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Organic and Biomolecular Chemistry | 2004

Schizophyllans carrying oligosaccharide appendages as potential candidates for cell-targeted antisense carrier.

Teruaki Hasegawa; Tomohisa Fujisawa; Munenori Numata; Takahiro Matsumoto; Mariko Umeda; Ryouji Karinaga; Masami Mizu; Kazuya Koumoto; Taro Kimura; Shiro Okumura; Kazuo Sakurai; Seiji Shinkai

Schizophyllans carrying beta-lactoside and alpha-mannoside appendages were prepared from native schizophyllan through NaIO4 oxidation followed by reductive amination using aminoethyl-beta-lactoside and alpha-mannoside, respectively. The resulting schizophyllans form stable macromolecular complexes with polynucleotides, such as poly(C) and poly(dA). Specific affinity between these macromolecular complexes and saccharide-binding proteins was demonstrated by surface plasmon resonance and agarose gel staining assays. beta-lactoside-appended schizophyllan enhanced an antisense activity in hepatocytes which express lactoside-binding proteins on their cell surfaces.

Langmuir | 2005

Inclusion of DNA into organic gelator fibers made of amphipathic molecules and its controlled release

Ryouji Karinaga; Yeonhwan Jeong; Seiji Shinkai; Kenji Kaneko; Kazuo Sakurai

Bioconjugate Chemistry | 2005

Transition from a normal to inverted cylinder for an amidine-bearing lipid/pDNA complex and its excellent transfection

Kazunori Koiwai; Kenji Tokuhisa; Ryouji Karinaga; Yasuya Kudo; Shota Kusuki; Yoichi Takeda; Kazuo Sakurai

Biomaterials | 2006

Galactose-PEG dual conjugation of β-(1→3)-d-glucan schizophyllan for antisense oligonucleotides delivery to enhance the cellular uptake

Ryouji Karinaga; Takahisa Anada; Jusaku Minari; Masami Mizu; Kazuya Koumoto; Junji Fukuda; Kohji Nakazawa; Teruaki Hasegawa; Munenori Numata; Seiji Shinkai; Kazuo Sakurai

Biomaterials | 2005

PEG-appended β-(1→3)-D-glucan schizophyllan to deliver antisense-oligonucleotides with avoiding lysosomal degradation

Ryouji Karinaga; Kazuya Koumoto; Masami Mizu; Takahisa Anada; Seiji Shinkai; Kazuo Sakurai

Journal of Controlled Release | 2005

Linear double-stranded DNA that mimics an infective tail of virus genome to enhance transfection.

Takahisa Anada; Ryouji Karinaga; Kazuya Koumoto; Masami Mizu; Takeshi Nagasaki; Yoshio Kato; Kazunari Taira; Seiji Shinkai; Kazuo Sakurai

Bulletin of the Chemical Society of Japan | 2004

Enhancement of the Antisense Effect of Polysaccharide-Polynucleotide Complexes by Preventing the Antisense Oligonucleotide from Binding to Proteins in the Culture Medium

Masami Mizu; Kazuya Koumoto; Takahisa Anada; Ryouji Karinaga; Taro Kimura; Takeshi Nagasaki; Seiji Shinkai; Kazuo Sakurai

Bioorganic & Medicinal Chemistry Letters | 2005

Schizophyllan-folate conjugate as a new non-cytotoxic and cancer-targeted antisense carrier

Teruaki Hasegawa; Tomohisa Fujisawa; Shuichi Haraguchi; Munenori Numata; Ryouji Karinaga; Taro Kimura; Shiro Okumura; Kazuo Sakurai; Seiji Shinkai

Bioorganic & Medicinal Chemistry Letters | 2006

CpG DNA/zymosan complex to enhance cytokine secretion owing to the cocktail effect

Takahisa Anada; Naoko Okada; Jusaku Minari; Ryouji Karinaga; Masami Mizu; Kazuya Koumoto; Seiji Shinkai; Kazuo Sakurai

Macromolecules | 2006

Polyrotaxane/DNA conjugate by use of intercalation: Bridge formation between DNA double helices

Kenji Tokuhisa; Emi Hamada; Ryouji Karinaga; Naohiko Shimada; Yoichi Takeda; Shinichi Kawasaki; Kazuo Sakurai

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Kazuo Sakurai

University of Kitakyushu

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Seiji Shinkai

Kyushu University

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Kazuya Koumoto

Konan University

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Masami Mizu

University of Kitakyushu

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Takahisa Anada

University of Kitakyushu

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Jusaku Minari

University of Kitakyushu

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Munenori Numata

Kyoto Prefectural University

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Taro Kimura

Kyushu University

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Takeshi Nagasaki

Kyushu University

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Teruaki Hasegawa

Kyushu University

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