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Dive into the research topics where Ryuhei Wakita is active.

Publication


Featured researches published by Ryuhei Wakita.


Applied and Environmental Microbiology | 2005

Purification and cDNA cloning of NADPH-dependent aldoketoreductase, involved in asymmetric reduction of methyl 4-bromo-3-oxobutyrate, from Penicillium citrinum IFO4631.

Hiroyuki Asako; Ryuhei Wakita; Kenji Matsumura; Masatoshi Shimizu; Jun Sakai; Nobuya Itoh

ABSTRACT Penicillium citrinum was found to catalyze the reduction of methyl 4-bromo-3-oxobutyrate to methyl (S)-4-bromo-3-hydroxybutyrate [(S)-BHBM] with high optical purity. From the strain, a cDNA clone encoding a novel NADPH-dependent alkyl 4-halo-3-oxobutyrate reductase (KER) was isolated. Escherichia coli cells overexpressing KER produced (S)-BHBM in the presence of an NADPH-regeneration system.


Archive | 2002

Method for producing optically active 2-hydroxycycloalkanecarboxylic acid ester

Hiroyuki Asako; Ryuhei Wakita; Nobuya Itoh


Archive | 2001

Process for producing optically active 4-halo-3-hydroxybutanoate

Hiroyuki Asako; Kenji Matsumura; Masatoshi Shimizu; Nobuya Itoh; Ryuhei Wakita


Archive | 2001

Process for producing 4-cyano-4oxobutanoate and 4-cyano-3-hydroxybutanoate

Nobuya Ito; Ryuhei Wakita


Archive | 2003

Process for producing 3-hydroxycyclohexanone

Nobuya Itoh; Ryuhei Wakita


Archive | 1996

Process for producing 2-fluorocyclopropanecarboxylic acid

Nobushige Itaya; Ryuhei Wakita; Asako Kubo; Mikio Sasaki; Takashi Namba; Yusuke Yukimoto


Archive | 2001

PROCESS FOR PREPARING HALOGENATED CYCLOPROPANE DERIVATIVES

Tsutomu Ebata; Toshifumi Akiba; Takanobu Ikeya; Ryuhei Wakita; Mikio Sasaki


Archive | 1998

Process for producing 2-fluorocyclopropancecarboxlic acid

Nobushige Itaya; Ryuhei Wakita; Asako Kubo; Mikio Sasaki; Takashi Namba; Yusuke Yukimoto


Archive | 2009

PROCESS FOR PRODUCTION OF PURIFIED O-(2,6-DICHLORO-4-METHYL-PHENYL) O,O-DIMETHYL PHOSPHOROTHIOATE

Eiji Yamauchi; Ryuhei Wakita


Archive | 2003

Verfahren zur Herstellung von 3-Hydroxycyclohexanon A process for producing 3-hydroxycyclohexanone

Nobuya Itoh; Ryuhei Wakita

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