Ryusuke Sudo

11-ketotestosterone Synchronously Induces Oocyte Development and Silvering-related Changes in the Japanese Eel, Anguilla japonica

To evaluate the effects of sex steroids on silvering in the Japanese eel, Anguilla japonica, the development of oocytes, eye size, digestive tract, and swim bladder were studied in relation to observations of the profiles of plasma levels of sex steroids (estradiol 17&bgr;, E2; testosterone, T; 11-ketotestosterone; 11-KT) during silvering for each sex and by administrating 11-KT to yellow eels. All steroids examined in the study increased in female eels after silvering had begun, whereas in males, only 11-KT increased significantly, and no statistical differences were found in plasma levels of E2 and T between eels in both developmental stages. 11-KT appeared to induce the early stage of oocyte growth, enlargement of the eyes, degeneration of the digestive tract and the development of the swim bladder. This suggested that 11-KT synchronously accelerates early development of the ovaries and the morphological changes, possibly in adaption to oceanic migration, and that 11-KT is one of the most important factors in early stages of development in the Japanese eel, as it appears to be in other anguillid eels.

Dynamics of Reproductive Hormones During Downstream Migration in Females of the Japanese Eel, Anguilla japonica

The profiles of sex steroids (estradiol-17&bgr;, testosterone and 11-ketotestosterone) and the mRNA levels of gonadotropins (luteinizing hormone and follicle-stimulating hormone) were investigated before and after downstream migration in females of the Japanese eel species Anguilla japonica, which were collected in the brackish Hamana Lake and its inlet freshwater rivers. Eels were separated into three groups using otolith microchemistry: ‘migrants’ that grew in the inlet rivers and then made a downstream migration to Hamana Lake mainly in October and November; ‘non-migrant’ yellow eels caught in rivers during the same season; and ‘residents,’ which were yellow eels caught in rivers in August. Sex steroid levels, especially those of testosterone and 11-ketotestosterone, were higher in migrants than in non-migrants and residents. Real-time quantitative PCR analysis indicated that mRNA levels of luteinizing hormone (LH) &bgr;-subunits were significantly higher in migrants than in other groups, whereas those of follicle-stimulating hormone &bgr;-subunits did not show significant changes during downstream migration. The high levels of these hormones during downstream migration raise the question about if they also play a role in motivating the migratory behavior of eels.

Profiles of mRNA expression for prolactin, growth hormone, and somatolactin in Japanese eels, Anguilla japonica: The effect of salinity, silvering and seasonal change

For understanding the functions of the growth hormone (GH)/prolactin (PRL)/somatolactin (SL) family of hormones, we examined pituitary mRNA expression of these hormones in anguillid eels in relation to salinity difference, silvering, and seasonal change. Female Japanese eels (Anguilla japonica) were collected in the brackish Hamana Lake and its freshwater rivers from July to December. To clarify the effect of salinity, the habitat use history of the eels were determined using otolith microchemistry. Expression levels of mRNA of each hormone were determined using real time PCR. Although GH and PRL have been known to be osmoregulatory hormones, there were no consistent differences in expression levels of these hormones between different salinity habitats. In contrast, SL mRNA expression was higher in eels from freshwater rivers than from the brackish lake. GH mRNA expression clearly decreased during silvering, whereas PRL and SL mRNA expression did not change. We also showed that PRL mRNA and SL mRNA decreased in the brackish lake and PRL mRNA increased in freshwater rivers from autumn to early winter. These findings provide basic knowledge for a further understanding of the role of these hormones.

Migratory Restlessness and the Role of Androgen for Increasing Behavioral Drive in the Spawning Migration of the Japanese eel

Migratory restlessness refers to a type of locomotor activity observed just before the onset of a migration. This behavior is primarily known in birds, where it is considered to be an indicator of the urge for migration. In contrast, little is known about migratory restlessness in fishes. To confirm migratory restlessness in a fish, we measured the locomotor activity of the Japanese eel, Anguilla japonica during its migration season. Migratory-phase silver eels showed higher locomotor activity in aquaria than yellow eels at the non-migratiory growth-phase. Silver eels stayed outside of their shelters for longer durations in dark periods than yellow eels and were active even in light periods when yellow eels were inactive in the shelters. Silver eels had higher levels of the androgen hormone 11-ketotestosterone at the end of experiment than yellow eels. Administration of 11-ketotesosterone to yellow eels induced higher levels of locomotor activity than that observed in non-treated controls. These findings suggest that anguillid eels exhibit migratory restlessness just before their spawning migration and that 11-ketotestosterone may be involved in the onset of this behavior.

Do Japanese eels recruit into the Japan Sea coast?: A case study in the Hayase River system, Fukui Japan

The Japanese coastlines along the Sea of Japan (Japan Sea) have been thought to be one of the margins of the distribution range of the Japanese eel Anguilla japonica, and there is evidence that eels had naturally recruited into these areas several hundred years ago. However, recruitment there is uncertain recently, because there seems to be no study that reported glass eel or elver recruitment into the coasts along the Japan Sea for a couple of decades, and the eels inhabiting these areas were probably stocked by fisheries cooperatives. In order to improve understanding of the present-day natural geographic distribution range of this species, we searched for naturally recruited wild eels in the Hayase River system, Fukui Prefecture, which flows into the Japan Sea. Multiple approaches including investigation of glass eel recruitment, comparison of body size, and estimation of habitat use types was employed. During the observation period (from January to July 2010), no glass eels were found at the river mouth of the Hayase River in monthly sampling. Of eels collected in this study (n = 127), no eels smaller than the initial body size of eels for stocking were found in this water system and none were identified as being of wild origin based on the habitat use type classifications from otolith microchemistry (n = 48). This lack of evidence of Japanese eels recruiting into Japan Sea coast waters suggests most eels present there may be stocked eels. Japanese eels could have been distributed naturally along the Japan Sea coast more than in recent years, indicating a possible decrease of the natural distribution range of this species.

Growth potential can affect timing of maturity in a long-lived semelparous fish

Many diadromous fishes such as salmon and eels that move between freshwater and the ocean have evolved semelparous reproductive strategies, but both groups display considerable plasticity in characteristics. Factors such as population density and growth, predation risk or reproduction cost have been found to influence timing of maturation. We investigated the relationship between female size at maturity and individual growth trajectories of the long-lived semelparous European eel, Anguilla anguilla. A Bayesian model was applied to 338 individual growth trajectories of maturing migration-stage female silver eels from France, Ireland, the Netherlands and Hungary. The results clearly showed that when growth rates declined, the onset of maturation was triggered, and the eels left their growth habitats and migrated to the spawning area. Therefore, female eels tended to attain larger body size when the growth conditions were good enough to risk spending extra time in their growth habitats. This flexible maturation strategy is likely related to the ability to use diverse habitats with widely ranging growth and survival potentials in the catadromous life-history across its wide species range.