S. De Nicola

Whole optical wavefields reconstruction by Digital Holography

In this paper, we have investigated on the potentialities of digital holography for whole reconstruction of wavefields. We show that this technique can be efficiently used for obtaining quantitative information from the intensity and the phase distributions of the reconstructed field at different locations along the propagation direction. The basic concept and procedure of wavefield reconstruction for digital in-line holography is discussed. Numerical reconstructions of the wavefield from digitally recorded in-line hologram patterns and from simulated test patterns are presented. The potential of the method for analysing aberrated wave front has been exploited by applying the reconstruction procedure to astigmatic hologram patterns.

Quantitative phase-contrast microscopy by a lateral shear approach to digital holographic image reconstruction

Combining the concept of lateral shear interferometry (LSI) within a digital holography microscope, we demonstrate that it is possible to obtain quantitative optical phase measurement in microscopy by a new single-image-processing procedure. Numerical lateral shear of the reconstructed wavefront in the image plane makes it possible to retrieve the derivative of the wavefront and remove the defocus aberration term introduced by the microscope objective. The method is tested to investigate a silicon structure and a mouse cell line.

A digital holographic microscope for complete characterization of microelectromechanical systems

Digital holographic microscopy (DHM) can be described as a non-invasive metrological tool for inspection and characterization of microelectromechanical structures (MEMS). DHM is a quick, non-contact and non-invasive technique that can offer a high resolution in both lateral and vertical directions. It has been employed for the characterization of the undesired out-of-plane deformations due to the residual stresses introduced by technological processes. The characterization of these deformations is helpful in studying and understanding the effect of residual stress on the deformation of a single microstructure. To that end, MEMS with different geometries and shapes, such as cantilever beams, bridges and membranes, have been characterized. Moreover, DHM has been applied efficiently to evaluate variations of the structure profile due to some external effects. As an example, the characterization of a cantilever subjected to a thermal process has been described. The results reported show that DHM is a useful non-invasive method for characterizing and developing reliable MEMS.

Liquid micro-lens array activated by selective electrowetting on lithium niobate substrates.

Lens effect was obtained in an open microfluidic system by using a thin layer of liquid on a polar electric crystal like LiNbO3. An array of liquid micro-lenses was generated by electrowetting effect in pyroelectric periodically poled crystals. Compared to conventional electrowetting devices, the pyroelectric effect allowed to have an electrode-less and circuit-less configuration. An interferometric technique was used to characterize the curvature of the micro-lenses and the corresponding results are presented and discussed. The preliminary results concerning the imaging capability of the micro-lens array are also reported.

Super-resolution in digital holography by a two-dimensional dynamic phase grating

An approach that uses an electro-optically tunable two dimensional phase grating to enhance the resolution in digital holographic microscopy is proposed. We show that, by means of a flexible hexagonal phase grating, it is possible to increase the numerical aperture of the imaging system, thus improving the spatial resolution of the images in two dimensions. The augment of the numerical aperture of the optical system is obtained by recording spatially multiplexed digital holograms. The grating tuneability allows one to adjust the intensity among the spatially multiplexed holograms maximizing the grating diffraction efficiency. Furthermore we demonstrate that the flexibility of the numerical reconstruction allows one to use selectively the diffraction orders carrying useful information for increasing the spatial resolution. The proposed approach can improve the capabilities of digital holography in three-dimensional imaging and microscopy.

Quantitative Phase Microscopy of microstructures with extended measurement range and correction of chromatic aberrations by multiwavelength digital holography

Quantitative Phase Microscopy (QPM) by interferometric techniques can require a multiwavelength configuration to remove 2pi ambiguity and improve accuracy. However, severe chromatic aberration can affect the resulting phase-contrast map. By means of classical interference microscope configuration it is quite unpractical to correct such aberration. We propose and demonstrate that by Digital Holography (DH) in a microscope configuration it is possible to clear out the QPM map from the chromatic aberration in a simpler and more effective way with respect to other approaches. The proposed method takes benefit of the unique feature of DH to record in a plane out-of-focus and subsequently reconstruct numerically at the right focal image plane. In fact, the main effect of the chromatic aberration is to shift differently the correct focal image plane at each wavelength and this can be readily compensated by adjusting the corresponding reconstruction distance for each wavelength. A procedure is described in order to determine easily the relative focal shift among different imaging wavelengths by performing a scanning of the numerical reconstruction along the optical axis, to find out the focus and to remove at the same time the chromatic aberration.

Optical properties of waveguides written by a 26 MHz stretched cavity Ti:sapphire femtosecond oscillator

We report on the fabrication, by a 26 MHz stretched-cavity femtosecond Ti:sapphire oscillator, of optical waveguides in different glass substrates, and their optical characterization. Operation of these waveguides in the telecom range at 1.55 microm is demonstrated. Digital holography microscopy is used to measure their refractive index profile. The results evidence a strong dependence of the fabrication process on the glass matrix.

Full Color 3-D Imaging by Digital Holography and Removal of Chromatic Aberrations

In color digital holography the recording and reconstruction of three holograms, by an optical setup operating in multi-wavelength mode, is necessary. Incorrect superposition of different images at various wavelengths may occur due to the presence of chromatic aberrations. We demonstrate that the compensation of the chromatic aberrations can be achieved by using all of the information contained in the multi-wavelength digital holograms. Chromatic aberration is extracted from phase maps reconstructed at different wavelengths, resulting in perfect full color amplitude reconstructions. The method has important applications in color 3-D holographic imaging and display.

Angular spectrum method with correction of anamorphism for numerical reconstruction of digital holograms on tilted planes

We present a new method for numerically reconstructing digital holograms on tilted planes. The method is based on the angular spectrum of plane waves. Fast Fourier transform algorithm is used twice and coordinate rotation in the Fourier domain enables to reconstruct the object field on the tilted planes. Correction of the anamorphism resulting from the coordinate transformation is performed by suitable interpolation of the spectral data. Experimental results are presented to demonstrate the method for a singleaxis rotation. The algorithm is especially useful for tomographic image reconstruction.

Tunable liquid microlens arrays in electrode-less configuration and their accurate characterization by interference microscopy

A special class of tunable liquid microlenses is presented here. The microlenses are generated by an electrowetting effect under an electrode-less configuration and they exhibit two different regimes that are named here as separated lens regime (SLR) and wave-like lens regime (WLR). The lens effect is induced by the pyroelectricity of polar dielectric crystals, as was proved in principle in a previous work by the same authors (S. Grilli et al., Opt. Express 16, 8084, 2008). Compared to that work, the improvements to the experimental set-up and procedure allow to reveal the two lens regimes which exhibit different optical properties. A digital holography technique is used to reconstruct the transmitted wavefront during focusing and a focal length variation in the millimetre range is observed. The tunability of such microlenses could be of great interest to the field of micro-optics thanks to the possibility to achieve focus tuning without moving parts and thus favouring the miniaturization of the optical systems.