S. Pedley

Risk factors contributing to microbiological contamination of shallow groundwater in Kampala, Uganda

A study of water quality variation in shallow protected springs in Kampala was undertaken over a 12-month period to assess the causes of microbiological contamination. The microbiological quality of water was assessed using thermotolerant coliforms and faecal streptococci. Sanitary inspections and hazard assessments were undertaken to identify faecal sources (hazards), contaminant pathways and contributory factors. Data were collected on rainfall and population as additional factors potentially exerting an influence on microbiological quality. Initial analysis of the data showed a significant relationship between median level of contamination and rainfall, in particular to short-term rainfall events. Total sanitary risk score showed a significant relationship with median level of contamination, but population density may be a confounding factor. The raw microbiological data were transformed into five water quality targets: <1 and < or =10 cfu 100ml(-1) for faecal streptococci; and <1, < or =10 and < or=50 cfu 100 ml(-1) for thermotolerant coliforms. The presence of individual risk factors as well as variables for rainfall and population density were analysed with respect to failure to meet these water quality targets using contingency tables. Logistic regression models were developed for each of the five water quality targets. The analysis strongly suggested that there is rapid recharge of the springs after rainfall and this leads to microbiological contamination. On-site sanitation was less important than other sources of faecal matter, which was consistent with a low sanitation coverage in the study area. The study suggested that improving sanitary completion and local environmental hygiene was more important than controlling on-site sanitation in improving the quality of these springs.

Marker species for identifying urban groundwater recharge sources: A review and case study in Nottingham, UK

Abstract Urban environments significantly alter the nature of recharge to underlying aquifers. Direct precipitation is reduced, but additional recharge may result from storm water runoff, mains supply leakage and sewer leakage. If urban aquifers are to be effectively and sustainably managed, it is vital that these recharge sources should be identified and quantified. A sound theoretical approach is the use of marker species for identifying the three principal sources of urban recharge (precipitation, mains and sewers). The ideal marker species should be unique to a particular recharge source (irrespective of geographic location), and easily identifiable in the groundwater system, enabling quantification of that source. A review of potential markers and a detailed study of the aquifer beneath the city of Nottingham, UK, was unable to find suitable markers for precipitation and mains leakage. Trihalomethanes, which are chlorination by-products, and so a potential marker of mains water, were hardly detected in either mains or groundwater. More potential markers are available for sewage, including d-limonene, which is a new ingredient in some detergents. For shallow groundwater, the most effective means of identifying sewage recharge was a combination of stable nitrogen isotopes and microbiological indicators; effectively a sewage “fingerprint”. This study confirms the need for a multi-component approach rather than using individual marker species. Additionally it demonstrates that the impact of sewer leakage on groundwater quality beneath Nottingham is generally not high.

The public health implications of microbiological contamination of groundwater

Abstract The microbiological contamination of groundwater has profound and severe implications for public health, particularly in small communities and developing countries where groundwater is often the preferred source of drinking water. Although natural groundwater is usually of good quality, this can deteriorate rapidly due to inadequate source protection and poor resource management. Contaminated groundwater can contribute to high morbidity and mortality rates from diarrhoeal diseases and sometimes lead to epidemics. The disposal of excreta using land-based systems is a key issue for groundwater quality and public health protection. The use of inappropriate water supply and sanitation technologies in peri-urban areas leads to severe and long-term public health risks. The use of poorly constructed sewage treatment works and land application of sewage can lead to groundwater contamination close to water supply sources. Microbiological, in particular virus survival in these circumstances is not well understood, but there are indications of extended pathogen survival and therefore increased public health risk.

In-situ tryptophan-like fluorescence: A real-time indicator of faecal contamination in drinking water supplies.

Enteric pathogens are typically inferred from the presence of surrogate indicator organisms such as thermotolerant (faecal) coliforms (TTCs). The analysis of TTCs requires time-consuming incubation in suitable laboratories, which can limit sampling resolution, particularly during critical pollution events. Here, we demonstrate the use of in-situ fluorimeters targeting tryptophan-like compounds as a rapid, reagentless indicator of TTCs in groundwater-derived potable water supplies in Africa. A range of other common indicators of TTCs were also determined including nitrate, turbidity, and sanitary risk survey scores. Sampling was conducted during both the dry and wet seasons to investigate seasonality. Tryptophan-like fluorescence was the most effective predictor of both presence/absence and number of TTCs during both seasons. Seasonal changes in tryptophan-like fluorescence in deeper supplies suggest it is transported more efficiently through the aquifer than TTCs. Moreover, the perennial elevated concentrations in some wells suggest it is more resilient than TTCs in groundwater. Therefore tryptophan-like fluorescence could also be a better indicator of some smaller, more easily transported, and long-lived, pathogenic enteric viruses. These sensors have the potential to be included in real-time pollution alert systems for drinking water supplies throughout the world, as well as for mapping enteric pathogen risks in developing regions.

On-site sanitation and urban aquifer systems in Uganda

Absence of microbial indicators from groundwater does not always mean absence of contamination. Research in sub-Saharan Africa warns against generalized assumptions and solutions.

Microbiological analysis of selected coastal bathing waters in the U.K., Greece, Italy and Spain

Abstract The study was undertaken to assess the performance of a selected range of holiday destinations against the microbiological determinants of the European Union (EU) bathing water Directive [Council directive of 8 December 1975 concerning the quality of bathing waters. Official Journal L,31, 1–7. 76/160/EEC.]. The destinations were situated in three Mediterranean countries —Greece, Spain and Italy— and in the U.K. itself. The Mediterranean destinations were chosen on the basis of their popularity with British visitors, the U.K. beaches on a fairly random basis. All the beaches in all countries were EU designated bathing waters, several holding the prestigious European Blue Flag award. Summary findings indicate that the five beaches sampled in Spain were all of very good microbiological quality for the ten days of sampling. Four of the beaches examined in Crete were of similar good quality. The fifth, at Malia, was of much poorer quality. The five Italian beaches all failed at least one of the Mandatory standards for either total or thermotolerant (faecal) coliforms. All these failures occurred on the same two days, coincident with heavy rainfall. The six U.K. beaches surveyed all passed Mandatory standards for total coliforms but one beach did not comply with the Mandatory standard for thermotolerant coliforms. The fact that several of the Mediterranean and U.K. beaches examined in the study were European Blue Flag beaches and that, on occasion, their water quality failed to meet the criteria for that award, suggests that such award schemes should clearly indicate that the guarantee of bather safety is far from absolute.

Urban groundwater quality in sub-Saharan Africa: current status and implications for water security and public health

Groundwater resources are important sources of drinking water in Africa, and they are hugely important in sustaining urban livelihoods and supporting a diverse range of commercial and agricultural activities. Groundwater has an important role in improving health in sub-Saharan Africa (SSA). An estimated 250 million people (40% of the total) live in urban centres across SSA. SSA has experienced a rapid expansion in urban populations since the 1950s, with increased population densities as well as expanding geographical coverage. Estimates suggest that the urban population in SSA will double between 2000 and 2030. The quality status of shallow urban groundwater resources is often very poor due to inadequate waste management and source protection, and poses a significant health risk to users, while deeper borehole sources often provide an important source of good quality drinking water. Given the growth in future demand from this finite resource, as well as potential changes in future climate in this region, a detailed understanding of both water quantity and quality is required to use this resource sustainably. This paper provides a comprehensive assessment of the water quality status, both microbial and chemical, of urban groundwater in SSA across a range of hydrogeological terrains and different groundwater point types. Lower storage basement terrains, which underlie a significant proportion of urban centres in SSA, are particularly vulnerable to contamination. The relationship between mean nitrate concentration and intrinsic aquifer pollution risk is assessed for urban centres across SSA. Current knowledge gaps are identified and future research needs highlighted.ResumeLes ressources en eaux souterraines sont des sources importantes d’eau potable en Afrique, et elles sont d’une énorme importance pour les êtres vivants en milieu urbain et pour le soutien d’une gamme diversifiée d’activités commerciales et agricoles. Les eaux souterraines jouent un rôle important en améliorant la santé en Afrique sub-saharienne (ASS). 250 millions de personnes environ (40% du total) vivent dans des centres urbains à travers l’ASS. L’ASS a connu une expansion rapide de ses populations urbaines depuis les années 1950, avec une augmentation à la fois des densités de population et de leur extension géographique. Les estimations prévoient un doublement de la population urbaine de l’ASS entre 2000 et 2030. L’état qualitatif des ressources en eau souterraine urbaines peu profondes est souvent très pauvre du fait de l’insuffisance de la gestion des déchets et de la protection de la ressource, et pose un risque sanitaire significatif pour les utilisateurs, alors que des ressources plus profondes captées par forage fournissent souvent une source importante d’eau potable de bonne qualité. Etant donné la croissance de la demande future de cette ressource limitée, ainsi que le changement climatique potentiel dans cette région dans le futur, une compréhension détaillée de la quantité et la qualité de cette ressource est exigée pour son utilisation durable. Cet article fournit une évaluation complète de l’état qualitatif, à la fois microbien et chimique, des eaux souterraines urbaines en ASS à travers une gamme de contextes hydrogéologiques et de différents types de point d’eaux souterraines. Les terrains du sous-sol de faible emmagasinement, qui sont situés sous une proportion significative de centres urbains en ASS, sont particulièrement vulnérables à la contamination. Le rapport entre la concentration moyenne en nitrate et le risque intrinsèque de pollution des aquifères est évalué pour les centres urbains de l’ASS. Des lacunes actuelles en matière de connaissances sont identifiées et les besoins de recherches futurs sont mis en évidence.ResumenLos recursos hídricos subterráneos son una fuente importante de agua potable en África y son sumamente importantes para mantener los medios de subsistencia urbanos y para apoyar una amplia gama de actividades comerciales y agrícolas. El agua subterránea tiene un papel importante en la mejora de la salud en el África Subsahariana (SSA). Se calcula que 250 millones de personas (40% del total) viven en centros urbanos en el África Subsahariana. El SSA ha experimentado una rápida expansión en las poblaciones urbanas desde la década de 1950, con el aumento de la densidad de población, así como la ampliación de la cobertura geográfica. Las estimaciones sugieren que la población urbana en África Subsahariana se duplicará entre 2000 y 2030. El estado de calidad del agua subterránea somera es a menudo muy pobre debido a la gestión inadecuada de los desechos y de la protección de las fuentes, representando un riesgo significativo para los usuarios, mientras que los pozos más profundos proporcionan una fuente importante de agua potable de buena calidad. Dado el crecimiento de la demanda futura de este recurso finito, así como los cambios potenciales en el clima futuro en esta región, una comprensión detallada de su cantidad y calidad es necesaria para utilizar este recurso de manera sostenible. Este artículo ofrece una evaluación integral del estado de la calidad del agua, tanto microbiana como química, del agua subterránea urbana en el SSA a lo largo de una serie de terrenos hidrogeológicos y diferentes tipos de puntos de agua subterránea. Los basamentos de bajo almacenamiento, que subyacen a una proporción significativa de los centros urbanos del África Subsahariana, son particularmente vulnerables a la contaminación. Se evalúa la relación entre la concentración media de nitratos y el riesgo intrínseco de contaminación acuífera para los centros urbanos a través de la SSA. Se identifican las deficiencias actuales de conocimientos y se destacan las necesidades de futuras investigaciones.摘要地下水资源是非洲重要的饮用水源,在维系城市生计和支撑各种商业和农业活动中至关重要。地下水在改善撒哈拉以南非洲民众健康方面发挥着重要作用。在撒哈拉以南非洲,预计有2.5亿人居住在城市地区(占总数的40 %)。自从上世纪50年代,本区域城市人口快速膨胀,密度不断增加,面积不断扩大。估测结果显示,撒哈拉以南非洲的城市人口在2000年至2030年间将翻一番。由于废物管理不当及水源污染,城市浅层地下水源水质常常非常差,对用水者产生很大的健康风险,而较深钻井水源常常提供重要的高质量饮水水源。考虑到此有限水源的基础上将来需求的增长以及本地区将来的气候潜在变化,需要详细了解水量和水质状况,以便可持续利用水源。本文从微生物和化学上综合评价了撒哈拉以南非洲各种水质状况地形及不同地下水水点类型的水质状况。在撒哈拉以南非洲,水存储量低的基岩地形在城市中心占有很大的比例,特别容易受到污染。对整个撒哈拉以南非洲的城市中心平均硝酸盐含量和固有的含水层污染风险之间的关系进行了评价。明确了目前的认识空白,并强调了将来的研究需求。ResumoAs águas subterrâneas são fontes importantes de água para consumo na África, e são amplamente importantes para sustentar o modo de vida urbano e dar suporte a uma amplitude diversa de atividades comerciais e agrícolas. As águas subterrâneas têm um papel importante no desenvolvimento da saúde na África subsaariana (ASS). São estimadas 250 milhões de pessoas (40% do total) vivendo nos centros urbanos na ASS. ASS tem vivenciado uma rápida expansão na população urbana desde os anos 50, com aumento das densidades populacionais assim como expansão da cobertura geográfica. As estimativas sugerem que a população urbana na ASS dobrará entre 2000 e 2030. As condições de qualidade dos aquíferos livres em áreas urbanas estão frequentemente muito pobres por causa do gerenciamento de perdas e proteção das fontes inadequados, e apresenta um risco significativo à saúde dos usuários, enquanto poços mais profundos frequentemente fornecem fontes importantes de água potável de boa qualidade. Dado o crescimento na demanda futura desse recurso finito, assim como mudanças potenciais no clima futuro da região, um entendimento detalhado da sua quantidade e qualidade é necessário para o uso sustentável deste recurso. Esse estudo fornece uma avaliação compreensiva das condições de qualidade da água, tanto microbiológica quanto química, das águas subterrâneas urbanas na ASS por uma gama de terrenos hidrogeológicos e diferentes tipos pontuais de águas subterrâneas. Terrenos de menor armazenamento de base, que fundamentam uma porção significante dos centros urbanos da ASS, são particularmente vulneráveis a contaminação. A relação entre a concentração média de nitrato e o risco intrínseco de poluição do aquífero é avaliado para os centros urbanos na ASS. Lacunas do conhecimento atual foram identificadas e destaca-se a necessidade de pesquisas futuras.

Tracing enteric pathogen contamination in sub-Saharan African groundwater

Quantitative PCR (qPCR) can rapidly screen for an array of faecally-derived bacteria, which can be employed as tracers to understand groundwater vulnerability to faecal contamination. A microbial DNA qPCR array was used to examine 45 bacterial targets, potentially relating to enteric pathogens, in 22 groundwater supplies beneath the city of Kabwe, Zambia in both the dry and subsequent wet season. Thermotolerant (faecal) coliforms, sanitary risks, and tryptophan-like fluorescence, an emerging real-time reagentless faecal indicator, were also concurrently investigated. There was evidence for the presence of enteric bacterial contamination, through the detection of species and group specific 16S rRNA gene fragments, in 72% of supplies where sufficient DNA was available for qPCR analysis. DNA from the opportunistic pathogen Citrobacter freundii was most prevalent (69% analysed samples), with Vibrio cholerae also perennially persistent in groundwater (41% analysed samples). DNA from other species such as Bifidobacterium longum and Arcobacter butzleri was more seasonally transient. Bacterial DNA markers were most common in shallow hand-dug wells in laterite/saprolite implicating rapid subsurface pathways and vulnerability to pollution at the surface. Boreholes into the underlying dolomites were also contaminated beneath the city highlighting that a laterite/saprolite overburden, as occurs across much of sub-Saharan aquifer, does not adequately protect underlying bedrock groundwater resources. Nevertheless, peri-urban boreholes all tested negative establishing there is limited subsurface lateral transport of enteric bacteria outside the city limits. Thermotolerant coliforms were present in 97% of sites contaminated with enteric bacterial DNA markers. Furthermore, tryptophan-like fluorescence was also demonstrated as an effective indicator and was in excess of 1.4μg/L in all contaminated sites.

Evaluation of an Inexpensive Growth Medium for Direct Detection of Escherichia coli in Temperate and Sub-Tropical Waters

The cost and complexity of traditional methods for the detection of faecal indicator bacteria, including E. coli, hinder widespread monitoring of drinking water quality, especially in low-income countries and outside controlled laboratory settings. In these settings the problem is exacerbated by the lack of inexpensive media for the detection of E. coli in drinking water. We developed a new low-cost growth medium, aquatest (AT), and validated its use for the direct detection of E. coli in temperate and sub-tropical drinking waters using IDEXX Quanti-Tray®. AT was compared with IDEXX Colilert-18® and either EC-MUG or MLSB for detecting low levels of E. coli from water samples from temperate (n = 140; Bristol, UK) and subtropical regions (n = 50, Pretoria/Tshwane, South Africa). Confirmatory testing (n = 418 and 588, respectively) and the comparison of quantitative results were used to assess performance. Sensitivity of AT was higher than Colilert-18® for water samples in the UK [98.0% vs. 86.9%; p<0.0001] and South Africa [99.5% vs. 93.2%; p = 0.0030]. There was no significant difference in specificity, which was high for both media (>95% in both settings). Quantitative results were comparable and within expected limits. AT is reliable and accurate for the detection of E. coli in temperate and subtropical drinking water. The composition of the new medium is reported herein and can be used freely.