S. Rajendran

Impact of silk fibroin-based scaffold structures on human osteoblast MG63 cell attachment and proliferation.

The present study was carried out to investigate the impact of various types of silk fibroin (SF) scaffolds on human osteoblast-like cell (MG63) attachment and proliferation. SF was isolated from Bombyx mori silk worm cocoons after degumming. Protein concentration in the degummed SF solution was estimated using Bradford method. Aqueous SF solution was used to fabricate three different types of scaffolds, viz, electrospun nanofiber mat, sponge, and porous film. The structures of the prepared scaffolds were characterized using optical microscopy and field emission scanning electron microscopy. The changes in the secondary structure of the proteins and the thermal behavior of the scaffolds were determined by Fourier transform infrared spectroscopy and thermo-gravimetric analysis, respectively. The biodegradation rate of scaffolds was determined by incubating the scaffolds in simulated body fluid for 4 weeks. MG63 cells were seeded on the scaffolds and their attachment and proliferation onto the scaffolds were studied. The MTT assay was carried out to deduce the toxicity of the developed scaffolds. All the scaffolds were found to be biocompatible. The amount of collagen produced by the osteoblast-like cells growing on different scaffolds was estimated.

Phase Competition Induced Bio-Electrochemical Resistance and Bio-Compatibility Effect in Nanocrystalline Zr-x-Cu100-x Thin Films

Nano-crystalline Zrx-Cu100-x (x = 20-100 at.%) thin films with thickness ranging from 50 to 185 nm were deposited by magnetron co-sputtering with individual Zr and Cu targets. The as-sputtered thin films were characterized by Field Emission Scanning Electron Microscope (FE-SEM), Atomic Force Microscopy (AFM) and Glancing Incidence X-ray Diffraction (GIXRD) for structural and morphological properties. The crystallite size was found to decrease from 57 nm to 37 nm upon increasing the Zr content from 20 to 30 at.% with slight increase in the lattice strain from 0.17 to 0.33%. Further, increase in Zr content to 40 at.% leads to increase in the crystallite size to 57 nm due to stabilization of C10Zr7 phase along with the presence of nanocrystalline Cu-Zr phase. A bimodal distribution of grain size was observed from FE-SEM micrograph was attributed to the highest surface roughness in Zr30Cu70 thin films comprised of Cu10Zr7, Cu9Zr2, Cu-Zr intermetallic phases. In-vitro electrochemical behaviors of nano-crystalline Zrx-Cu100-x thin films in simulated body fluid (SBF) were investigated using potentiodynamic polarization studies. Electrochemical impedance spectroscopy (EIS) data fitting by equivalent electrical circuit fit model suggests that inner bulk layer contributes to high bio-corrosion resistance in Zrx-Cu100-x thin films with increase in Zr content. The results of cyto-compatibility assay suggested that Zr-Cu thin film did not introduce cytotoxicity to osteoblast cells, indicating its suitability as a bio-coating for minimally invasive medical devices.

Stimulation of human osteoblast cells (MG63) proliferation using decanoic acid and isopropyl amine fractions of Wattakaka volubilis leaves

This study was carried out to investigate the impact of various isolated phytochemical components present in the Wattakaka volubilis leaves for the growth and proliferation of human osteoblast like cells (MG63).

Development of Colorimetric Cholesterol detection kit using TPU Nanofiber/ Cellulose Acetate membrane

In this study, the authors report a simple fabrication of thermoplastic polyurethane (TPU) nanofibres-based kit for cholesterol detection. TPU is a polymer that is highly elastic, resistant to microorganisms, abrasion and compatible with blood; thus, making it a natural selection as an immobilisation matrix for cholesterol oxidase (ChOx) enzyme. The nanofibre was fabricated by electrospinning process and was characterised using scanning electron microscopy and Fourier transform-infrared spectroscopy. ChOx was covalently immobilised on TPU nanofibre and cholesterol level/concentration was visually found using 4-aminoantipyrine, a dye that reacts with H2O2 produced from the oxidation of cholesterol by ChOx and changes colour from yellow to red. The efficacy of the nanofibre to act as a detecting substrate was compared with cellulose acetate (CA) membrane, a well-documented enzyme immobilisation matrix. The optimisation of enzyme concentration and dye quantity were performed using standard ChOx spectrophotometric assay and the same was used in CA membrane and TPU nanofibre. The ChOx immobilised nanofibre showed good linear range from 2 to 10 mM with a lower detection limit of 2 mM and was highly stable compared to that of CA membrane. The enzyme immobilised nanofibre was further validated in serum samples.