S. Rossini Oliva

Accumulation and in vivo tissue distribution of pollutant elements in Erica andevalensis.

Erica andevalensis is an endemic shrub from an area in the southwest of Spain (Andalucia) characterized by acidic and contaminated soils. Scanning electron microscopy (SEM) of samples after conventional or cryo-fixation preparation protocols was used for morphological and anatomical studies. SEM coupled with EDX-analysis was employed to localise and quantify different elements within plant parts (leaves, stems and roots) in samples collected in the field. Morphological studies revealed that the species has typical adaptive structures to drought-stress such as rolled needle-like leaves, sunken stomata and a thick waxy cuticle on the upper epidermis. Roots were associated with fungi which formed intra and extra-cellular mycelia. The SEM studies showed that Cu was not sequestrated into the root tissues and was uniformly distributed in leaf tissues. Meanwhile, Pb was only localised within epidermal root tissues which indicates that its sequestration in an external matrix might represent a tolerance mechanism in this species. Iron was uniformly distributed throughout the leaves, while in roots it was predominantly retained on the epidermal cell walls. The exclusion and tolerance mechanisms adopted by this species to survive in mining areas indicate that it can be used successfully in the re-vegetation of contaminated areas.

Stress responses of Erica andevalensis Cabezudo & Rivera plants induced by polluted water from Tinto River (SW Spain).

The effects of Tinto River water on Erica andevalensis growth, biochemical indicators and elemental concentration and distribution were investigated under laboratory conditions. High levels of toxic elements such as B, Fe and S and acidic pH characterized the river water. Plant analysis revealed that the concentration of Al, B, S and Fe increased in all plant organs reaching in some cases values in the toxicity range. Plants transferred into river water stopped growing and stress was manifested by plant water loss, increase in peroxidase activity and decrease of chlorophyll a concentration. Significant decreases of free amino acid concentration were found in shoots and roots of plants grown in diluted river water. The results indicated that Tinto River water acidity and its excess in soluble elements produced altogether severe alterations in roots affecting plant water and nutrient uptake and leading to the massive entry of some metals (e.g. Fe, Al) with toxic effects. Scanning-electron microscopy (cryoSEM and ESEM) observations showed that E. andevalensis had not exclusion mechanisms of Cu, Fe and S therefore it was not able to reduce translocation to aerial parts.

Evaluation of Lead Toxicity in Erica andevalensis as an Alternative Species for Revegetation of Contaminated Soils

Although revegetation using native flora is a low cost way to stabilize soil and restore the landscape contaminated with metals, little is known regarding the Pb-tolerance of many of these species. For this purpose, we evaluated the tolerance of Erica andevalensis to Pb by growing plants in nutrient solutions with increasing concentrations of Pb (up to 100 μM). Plant growth and different physiological parameters were determined to ascertain tolerance to metal stress. Additionally, an electron microscopy study coupled with EDX-analysis was performed to get clues on the Pb uptake and translocation from roots into stem and leaves. The LOEC (the lowest observed effect concentration) of Pb was 40 μM while the IC50 (inhibition concentration) was 80 μM Pb. Chemical analysis revealed a root>stem>leaf accumulation pattern. There was a severe reduction in fresh biomass and chlorophyll concentration at the highest Pb dose. The SEM-EDX study indicated that Pb was mostly located in root epidermal tissues. The blockage of Pb on the root probably avoided its toxic effects by limiting Pb transport to other tissues.

Response of drought and fertilization in Erica andevalensis seed banks: Significance for conservation management

The present study attempts to investigate the size, composition and seedling dynamics of the seed bank of a metalliferous and vulnerable species, Erica andevalensis. Samples were taken during spring and autumn from two different sites. We also studied the effects of nutrient solution, irrigation from the river Tinto and irrigation deficit on seeding establishment and survival. Only E. andevalensis and Poaceae species emerged from the seed banks, although the former was dominant (98%). Germination and seedling establishment was totally inhibited by the waters of the river Tinto. Seed density was high in the soils of both seed banks irrigated with water and nutrient solution. We found no seasonal differences in the seed bank and number of germinated seeds and mortality rate and density were similar. From the standpoint of restoration management, the results indicate that the seed bank is a very important factor for successful species establishment.