Network


Latest external collaboration on country level. Dive into details by clicking on the dots.

Hotspot


Dive into the research topics where S Tzartos is active.

Publication


Featured researches published by S Tzartos.


FEBS Letters | 1983

Demonstration of a main immunogenic region on acetylcholine receptors from human muscle using monoclonal antibodies to human receptor

S Tzartos; Lorene Langeberg; Susan Hochschwender; Jon Lindstrom

Eleven cloned hybridomas which secrete antibodies to acetylcholine receptors from human muscle have been prepared. All of these monoclonal antibodies to have the same basic specificity as shown by competition for binding to the main immunogenic region on the receptor, but these antibodies differ in fine specificity as shown by reaction with denatured receptor subunits and interspecies cross‐reaction.


Annals of the New York Academy of Sciences | 1981

STRUCTURE AND FUNCTION OF THE ACETYLCHOLINE RECEPTOR MOLECULE STUDIED USING MONOCLONAL ANTIBODIES

Jon Lindstrom; S Tzartos; William Gullick

Acetylcholine receptors ( AChRs) are the object of an antibody-mediated autoimmune response in myasthenia gravis (MG) and its animal model, experimental autoimmune myasthenia gravis (EAMG) (reviewed in references 1-4). In order to understand how AChR might provoke an immune response or respond to an immune attack, it is important to understand the structure and function of the AChR molecule. And, in order to understand how antibodies to the AChR act, it is important to determine what specificities of antibodies can be made and what effect they have on the AChR. The structure and function of the AChR are also of fundamental interest, because it is the archetypic neurotransmitter receptor and a model surface membrane protein. Antisera containing heterogeneous populations of antibodies are useful reagents, but pure antibodies of each type would be better. When an animal is immunized with AChR it makes a large population of antibodies directed at many determinants on the molecule. As a result of differences in their “variable” regions, these antibodies differ in which antigenic determinants they bind and in the affinity with which they bind to these determinants; and as a result of differences in their “constant” regions, these antibodies differ in their ability to interact with other immune effectors like complement and phagocytes. Several aspects of AChR structure, function and the pathology of MG could be very effectively studied using homogeneous antibody reagents. For example, most antibodies bind to AChRs without affecting the acetylcholine binding sites or the cation channel they reg~late.~--” Such antibodies, if obtained as individual reagents, would be excellent probes for structures on the AChR macromolecule other than the acetylcholine binding site. The acetylcholine binding site comprises only a tiny part of the extracellular surface of this large macromolecule, and is in no great need of probes, since snake venom toxins,1° affinity labeling reagents,11 and reversible agonists and antagonists are available in abundance. Some antibodies in anti-AChR sera can effect the length of time that the cation channel triggered by acetylcholine is open or its conductance when open.?, 12 Such antibodies, if obtained as individual reagents, would be excellent probes for the AChR channel, for which few other probes exist. LOSS


Proceedings of the National Academy of Sciences of the United States of America | 1980

Monoclonal antibodies used to probe acetylcholine receptor structure: localization of the main immunogenic region and detection of similarities between subunits

S Tzartos; Jon Lindstrom


Journal of Biological Chemistry | 1981

Mapping of surface structures of electrophorus acetylcholine receptor using monoclonal antibodies.

S Tzartos; D E Rand; Brett Einarson; Jon Lindstrom


Proceedings of the National Academy of Sciences of the United States of America | 1982

Specificities of antibodies to acetylcholine receptors in sera from myasthenia gravis patients measured by monoclonal antibodies.

S Tzartos; Marjorie E. Seybold; Jon Lindstrom


Journal of Biological Chemistry | 1982

Inhibition of glycosylation with tunicamycin blocks assembly of newly synthesized acetylcholine receptor subunits in muscle cells.

John P. Merlie; R Sebbane; S Tzartos; Jon Lindstrom


Proceedings of the National Academy of Sciences of the United States of America | 1983

Immunohistochemical localization of monoclonal antibodies to the nicotinic acetylcholine receptor in chick midbrain

Larry W. Swanson; Jon Lindstrom; S Tzartos; L C Schmued; D D O'Leary; W. M. Cowan


Cold Spring Harbor Symposia on Quantitative Biology | 1983

Use of monoclonal antibodies to study acetylcholine receptors from electric organs, muscle, and brain and the autoimmune response to receptor in myasthenia gravis.

Jon Lindstrom; S Tzartos; William Gullick; Susan Hochschwender; Larry W. Swanson; Peter B. Sargent; Michele H. Jacob; Mauricio Montal


Journal of Biological Chemistry | 1983

Characterization of the mRNA for mouse muscle acetylcholine receptor alpha subunit by quantitative translation in vitro.

R Sebbane; G Clokey; John P. Merlie; S Tzartos; Jon Lindstrom


Archive | 2016

Monoclonal antibodies used to probe acetylcholine receptor structure: Localization of the main immunogenic region and detection of similarities between subunits (interspecies crossreaction/muscle/experimental autoimmune myasthenia gravis)

S Tzartos; Jon Lindstrom

Collaboration


Dive into the S Tzartos's collaboration.

Top Co-Authors

Avatar

Jon Lindstrom

University of Pennsylvania

View shared research outputs
Top Co-Authors

Avatar

William Gullick

Salk Institute for Biological Studies

View shared research outputs
Top Co-Authors

Avatar

John P. Merlie

Washington University in St. Louis

View shared research outputs
Top Co-Authors

Avatar

Larry W. Swanson

University of Southern California

View shared research outputs
Top Co-Authors

Avatar

Susan Hochschwender

Salk Institute for Biological Studies

View shared research outputs
Top Co-Authors

Avatar

Brett Einarson

Salk Institute for Biological Studies

View shared research outputs
Top Co-Authors

Avatar

L C Schmued

Salk Institute for Biological Studies

View shared research outputs
Top Co-Authors

Avatar

Lorene Langeberg

Salk Institute for Biological Studies

View shared research outputs
Top Co-Authors

Avatar

Marjorie E. Seybold

Salk Institute for Biological Studies

View shared research outputs
Top Co-Authors

Avatar
Researchain Logo
Decentralizing Knowledge